Immunological method for direct assessment of the functionality of a denitrifying strain of Pseudomonas fluorescens in soil

Journal of Microbiological Methods

Volumn 58, Issue 1, 2004, Pages 13-21

  Maron, P A ^a,b   Richaume, A ^a   Potier, P ^a   Lata, J C ^a,c   Lensi, R ^a,d  

^a University of Lyon   (France)

^b CEMAGREF   (France)

^c UNIVERSITÉ PARIS SUD   (France)

^d CENTRE D ECOLOGIE FONCTIONNELLE ET EVOLUTIVE   (France)

Author keywords

Chemifluorescence; Complex environments; Dissimilative nitrate reductase; Enzyme quantification; Immunological method

Indexed keywords

NITRATE; NITRATE REDUCTASE; NITRITE; POLYCLONAL ANTIBODY;

ANTIBODY PRODUCTION; ARTICLE; BACTERIUM CULTURE; CATALYSIS; CATALYST; CONTROLLED STUDY; DENITRIFICATION; ECOSYSTEM; ENZYME PURIFICATION; FLUORESCENCE; IMMUNOLOGICAL TECHNIQUE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PSEUDOMONAS FLUORESCENS; REDUCTION; REPRODUCIBILITY; SOIL MICROFLORA; WESTERN BLOTTING;

ANTIBODIES, BACTERIAL; ANTIBODY SPECIFICITY; COLONY COUNT, MICROBIAL; IMMUNOBLOTTING; NITRATE REDUCTASE; NITRATE REDUCTASES; PSEUDOMONAS FLUORESCENS; SOIL MICROBIOLOGY;

PSEUDOMONAS; PSEUDOMONAS FLUORESCENS;

EID: 2642585515     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2004.02.013     Document Type: Article

References (27)

1. Baumann B., Snozzi M., Zehnder A.J.B., van der Meer J.R. Dynamics of denitrification activity of Paracoccus denitrificans in continuous culture during aerobic-anaerobic changes. J. Bacteriol. 178:1996;4367-4374
2. Baumann B., Snozzi M., van der Meer J.R., Zehnder A.J.B. Development of stable denitrifying cultures during repeated aerobic-anaerobic transient periods. Water Res. 31:1997;1947-1954
3. Bock E., Koops H.-P. The genus Nitrobacter and related genera. Balows A., Trüper H.G., Dworkin M., Harder W., Schleifer K.-H. The Prokaryotes III. 1992;2302-2309 Springer-Verlag, Berlin
4. Bock E., Sundermeyer-Klinger H., Stackebrandt E. New facultative lithoautotrophic nitrite-oxidizing bacteria. Arch. Microbiol. 136:1983;281-284
5. de Boer W., Kowalchuk G.A. Nitrification in acid soils: micro-organisms and mechanisms. Soil Biol. Biochem. 33:2000;853-866
6. Ghiglione J.-F., Gourbière F., Potier P., Lensi R. Role of the respiratory nitrate reductase in the ability of Pseudomonas fluorescens YT101 to colonise the rhizosphere of maize. Appl. Environ. Microbiol. 66:2000;4012-4016
7. Hobbie J.E., Daley R.J., Jasper S. Use of nucleopore filters for counting bacteria by fluorescence microscopy. Appl. Environ. Microbiol. 33:1977;1225-1228
8. Koops H.P., Pommerening-Röser A. Distribution and ecophysiology of the nitrifying bacteria emphasizing cultured species. FEMS Microbiol. Ecol. 37:2001;1-9
9. Körner H., Zumft W.G. Expression of denitrification enzymes in response to the dissolved oxygen level and respiratory substrate in continuous culture of Pseudomonas stuzeri. Appl. Environ. Microbiol. 55:1989;1670-1676
10. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685
11. Latour X., Philippot L., Corberand T., Lemanceau P. The establishment of an introduced community of fluorescent pseudomonads in the soil and in the rhizosphere is affected by the soil type. FEMS Microbiol. Ecol. 30:1999;163-170
12. Lensi R., Clays-Josserand A., Jocteur Monrozier L. Denitrifiers and denitrifying activity in size fractions of a mollisol under permanent pasture or continuous cultivation. Soil Biol. Biochem. 27:1995;61-69
13. Lindahl V., Bakken L.R. Evaluation of methods for extraction of bacteria from soil. FEMS Microbiol. Ecol. 16:1995;135-142
14. Maron P.-A., Coeur C., Pink C., Clays-Josserand A., Lensi R., Richaume A., Potier P. Use of polyclonal antibodies to detect and quantify the NOR protein of nitrite oxidizers in complex environments. J. Microbiol. Methods. 53:2003;87-95
15. Martin K., Parsons L.L., Murray R.E., Smith S. Dynamics of soil denitrifier populations: relationships between enzyme activity, Most-Probable-Number counts, and actual N gas loss. Appl. Environ. Microbiol. 54:1988;2711-2716
16. Ogunseitan O.A. Direct extraction of catalytic proteins from natural microbial communities. Cell. Mol. Life Sci. 58:1997;215-224
17. Otte S., Grobben N.G., Robertson L.A., Jetten M.S.M., Kuenen J.G. Nitrous oxide production by Alcaligenes faecalis under transient and dynamic aerobic and anaerobic conditions. Appl. Environ. Microbiol. 62:1996;2421-2426
18. Philippot L., Clays-Josserand A., Lensi R., Trinsoutrot I., Normand P., Potier P. Purification of the dissimilative nitrate reductase of Pseudomonas fluorescens and the cloning and sequencing of its corresponding genes. Biochim. Biophys. Acta. 1350:1997;272-276
19. Philippot L., Clays-Josserand A., Lensi R. Use of Tn5 mutants to assess the role of the dissimilatory nitrite reductase in the competitive abilities of two Pseudomonas strains in soil. Appl. Environ. Microbiol. 61:1995;1426-1430
20. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd ed. 1989;Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
21. Thomas K.L., Lloyd D., Boddy L. Effects of oxygen, pH and nitrate concentration on denitrification by Pseudomonas species. FEMS Microbiol. Lett. 118:1994;181-186
22. Tiedje J.M. Denitrification. Page A.L., Miller R.H., Keeney D.R. Methods of Soil Analysis. Part 2 - Chemical and Microbiological Properties, Pt 2. Agron. Monogr. vol. 9:1982;1011-1024 American Society of Agronomy, Madison, WI, USA
23. Torsvik V., Øvreås L. Microbial diversity and function in soil: from gene to ecosystems. Curr. Opin. Microbiol. 5:2002;240-245
24. Vinther F.P., Memon H.G., Jensen V. Populations of denitrifying bacteria in agricultural soil under continuous barley cultivation. Pedobiology. 24:1982;319-328
25. Volz M.G. Assessing two diagnostic methods for enumeration of nitrate dissimilators. Soil Sci. Am. J. 41:1977;337-340
26. Waibel A.E., Peter T., Carslaw K.S., Oelhaf H., Wetzel G., Crutzen P.J., Poschl U., Tsias A., Reimer E., Fisher H. Arctic ozone loss due to denitrification. Science. 283:1999;2064-2069
27. Ye R.W., Arunakumari A., Averill A., Tiedje J.M. Mutants of Pseudomonas fluorescens deficient in dissimilatory nitrite reductase are also altered on nitric oxide reduction. J. Bacteriol. 174:1992;2560-2564