Development and validation of a PCR-based protocol for the detection of Pseudomonas anguilliseptica

Fish Pathology

Volumn 39, Issue 1, 2004, Pages 33-41

  Romalde, Jesús L ^a   López Romalde, Sonia ^a   Ravelo, Carmen ^a   Magariños, Beatriz ^a   Toranzo, Alicia E ^a  

^a UNIVERSITY OF SANTIAGO DE COMPOSTELA   (Spain)

Author keywords

16S rDNA; Diagnosis; PCR detection; Pseudomonas anguilliseptica

Indexed keywords

BACTERIA (MICROORGANISMS); PSEUDOMONAS; PSEUDOMONAS ANGUILLISEPTICA; SCOPHTHALMUS; SCOPHTHALMUS MAXIMUS; SPARUS; SPARUS AURATA;

EID: 2642575208     PISSN: 0388788X     EISSN: None     Source Type: Journal    
DOI: 10.3147/jsfp.39.33     Document Type: Article

References (18)

1. Al-Marzouk, A. E. (1999): Association of Pseudomonas anguilliseptica with mortalities in cultured marine orange-spotted grouper Epinephelus coioides in Kuwait. Fish Pathol., 34, 167-168.
2. Berthe, F. C. J., C. Michel and J.-F. Bernardet (1995): Identification of Pseudomonas anguilliseptica isolated from several fish species in France. Dis. Aquat. Org., 21, 151-155.
3. Blanco, M. M., A. Gibello, A. I. Vela, M. A. Moreno, L. Domínguez and J. F. Fernández-Garayzábal (2002): PCR detection and PFGE DNA macrorestriction analysis of clinical isolates of Pseudomonas anguilliseptica from winter disease outbreaks in sea bream Sparus aurata. Dis. Aquat. Org., 50, 19-27.
4. Daly, J. G. (1999): Other bacterial pathogens. In "Fish diseases and disorders. Viral, bacterial and fungal infections" (ed. by P. T. K. Woo and D. W. Bruno). CABI Publishing, New York, pp. 577-598.
5. Doménech, A., J. F. Fernéndez-Garayzabal, P. Lawson, J. A. García, M. T. Cutuli, M. Blanco, A. Gibello, M. A. Moreno, M. D. Collins and L. Domínguez (1997): Winter disease outbreak in sea bream (Sparus aurata) associated with Pseudomonas anguilliseptica infection. Aquaculture, 156, 317-326.
6. Hiney, M. and P. Smith (1998): Validation of polymerase chain reaction-based detection techniques for bacterial pathogens in the environment: Framework, problems and possible solutions. Aquaculture, 162, 41-68.
7. Kusuda, R., N. Dohata, Y. Fukuda and K. Kawai (1995): Pseudomonas anguilliseptica infection of striped jack. Fish Pathol., 30, 121-122.
8. Lönnström, L., T. Wiklund and G. Bylund (1994): Pseudomonas anguilliseptica isolated from Baltic herring Clupea harengus membras with eye lesions. Dis. Aquat. Org., 18, 143-147.
9. López-Romalde, S., B. Magariños, S. Núñez, A. E. Toranzo and J. L. Romalde (2003): Phenotypic and genetic characterization of Pseudomonas anguilliseptica strains isolated from fish. J. Aquat. Anim. Health, 15, 39-47.
10. Michel, C., J.-F. Bernardet and D. Dinand (1992): Phenotypic and genotypic studies of Pseudomonas anguilliseptica strains isolated from farmed European eels (Anguilla anguilla) in France. Fish Pathol., 27, 229-232.
11. Nakai, T, K. Muroga and H. Wakabayashi (1985): First records of Pseudomonas anguilliseptica infection in cultured ayu, Plecoglossus altivelis. Fish Pathol., 20, 481-484.
12. Osorio, C. R., M. D. Collins, A. E. Toranzo, J. L. Barja and J. L. Romalde (1999): 16S rRNA gene sequence analysis of Photobacterium damselae and nested PCR method for rapid detection of the causative agent of fish pasteurellosis. Appl. Environ. Microbiol., 65, 2942-2946.
13. Padrós, F., A. Hern·ndez, J. Rotllant, M. Puigcerver, R. Sala, S. Crespo, L. Tort, A. Ibarz, M. Sala, M. A. Gallardo, J. Blasco, J. Fernández and J. Sánchez (1995): La enfermedad del invierno en la dorada Sparus aurata. Características del síndrome, disfunciones observadas y metodología de análisis y prevención. VII Congreso Nacional de Acuicultura. Las Palmas de Gran Canaria, Spain. pp. 169.
14. Reed, L. J. and H. Müench (1938) A simple method of estimating fifty percent end points. Am. J. Hyg., 27, 493-497.
15. Romalde, J. L. and A. E. Toranzo (2002): Molecular approaches for the study and diagnosis of salmonid streptococcosis. In "Molecular diagnosis of salmonid diseases" (ed. by C. Cunningham). Kluwer Academic Publishers, Dordrecht, The Netherlands. pp. 211-233.
16. Romalde, J. L., S. López-Romalde, C. Ravelo, B. Magariños and A. E. Toranzo (2003): Evidences of two clonal lineages within the emerging fish pathogen Pseudomonas anguilliseptica by serological and genetic techniques. AFS/FHS Newslett, 31, 8-9.
17. Wakabayashi, H. and S. Egusa (1972): Characteristics of a Pseudomonas sp. from an epizootic of pond-cultured eels (Anguilla japonica). Bull. Japan. Soc. Sci. Fish., 38, 577-587.
18. Wiklund, T. and G. Bylund (1990): Pseudomonas anguilliseptica as a pathogen of salmonid fish in Finland. Dis. Aquat. Org., 8, 13-19.