Comparison of PCR detection of mecA with agar dilution and etest for oxacillin susceptibility testing in clinical isolates of coagulase-negative staphylococci

Clinical Microbiology and Infection

Volumn 10, Issue 5, 2004, Pages 462-465

  Tveten, Y ^a,b   Jenkins, A ^a   Digranes, A ^c   Melby, K K ^d   Allum, A G ^a   Kristiansen, B E ^a,b  

^a AS Telelab   (Norway)

^b UNIVERSITY OF TROMSØ   (Norway)

^c HAUKELAND UNIVERSITY HOSPITAL   (Norway)

^d ULLEVÅL UNIVERSITY HOSPITAL   (Norway)

Author keywords

Agar dilution; Coagulase negative staphylococci; Etest; Oxacillin resistance; PCR; Susceptibility testing

Indexed keywords

BETA LACTAM ANTIBIOTIC; OXACILLIN; PENICILLIN BINDING PROTEIN 2A;

ACCURACY; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIUM ISOLATE; COAGULASE NEGATIVE STAPHYLOCOCCUS; CONTROLLED STUDY; CORRELATION ANALYSIS; DILUTION; DRUG SCREENING; INTERMETHOD COMPARISON; LABORATORY TEST; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SCREENING TEST; SENSITIVITY AND SPECIFICITY; STANDARDIZATION; STAPHYLOCOCCUS EPIDERMIDIS; STAPHYLOCOCCUS HOMINIS;

STAPHYLOCOCCI;

EID: 2642572047     PISSN: 1198743X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1469-0691.2004.00879.x     Document Type: Article

References (20)

1. Kloos WE, Bannermann TL. Update on clinical significance of coagulase-negative staphylococci. Clin Microbiol Rev 1994; 7: 117-140.
2. Marshall SA, Wilke WW, Pfaller MA, Jones RN. Staphylococcus aureus and coagulase-negative staphylococci from blood stream infections: frequency of occurrence, antimicrobial susceptibility, and molecular (mecA) characterization of oxacillin resistance in the SCOPE program. Diagn Microbiol Infect Dis 1998; 30: 205-212.
3. von Eiff C, Peters G, Heilmann C. Pathogenesis of infections due to coagulase-negative staphylococci. Lancet Infect Dis 2002; 2: 677-685.
4. Chambers HF. Methicillin-resistant staphylococci. Clin Microbiol Rev 1988; 1: 173-186.
5. Archer GL, Pennell E. Detection of methicillin resistance in staphylococci by using a DNA probe. Antimicrob Agents Chemother 1990; 34: 1720-1724.
6. Olsson-Liljequist B, Larsson P, Ringertz S, Løfdahl S. Use of a DNA hybridization method to verify results of screening for methicillin resistance in staphylococci. Eur J Clin Microbiol Infect Dis 1993; 12: 527-533.
7. Jarløv JO, Bosch-Sørensen C, Espersen F, Mortensen I, Hougaard DM, Rosdahl VT. Evaluation of different methods for the detection of methicillin-resistance in coagulase-negative staphylococci. J Antimicrob Chemother 1997; 40: 241-249.
8. McDonald CL, Maher WE, Fass RJ. Revised interpretation of oxacillin MICs for Staphylococcus epidermidis based on mecA detection. Antimicrob Agents Chemother 1995; 39: 982-984.
9. Predari SC, Ligozzi M, Fontana R. Genotypic identification of methicillin-resistant coagulase-negative staphylococci by polymerase chain reaction. Antimicrob Agents Chemother 1991; 35: 2568-2573.
10. Weller TM, Crook DW, Croow MR, Ibrahim W, Pennington TH, Selkon JB. Methicillin susceptibility testing of staphylococci by E test and comparison with agar dilution and mecA detection. J Antimicrob Chemother 1997; 39: 251-253.
11. Tenover FC, Jones RN, Swenson JM et al. Methods for improved detection of oxacillin resistance in coagulase negative staphylococci: results of a multicenter study. J Clin Microbiol 1999; 37: 4051-4058.
12. Kohner P, Uhl J, Kolbert C, Persing D, Cockerill F. Comparison of susceptibility testing methods with mecA gene analysis for determining oxacillin (methicillin) resistance in clinical isolates of Staphylococcus aureus and coagulase-negative Staphylococcus spp. J Clin Microbiol 1999; 37: 2952-2961.
13. Graham JC, Murphy OM, Stewart D, Kearns AM, Galloway A, Freeman R. Comparison of PCR detection of mecA with methicillin and oxacillin disc susceptibility testing in coagulase-negative staphylococci. J Antimicrob Chemother 2000; 45: 111-113.
14. Hussain Z, Stoakes L, Massey V et al. Correlation of oxacillin MIC with mecA gene carriage in coagulase-negative staphylococci. J Clin Microbiol 2000; 38: 752-754.
15. Gradelski E, Valera L, Aleksunes L, Bonner D, Fung-Tomc J. Correlation between genotype and phenotypic categorization of staphylococci based in methicillin susceptibility and resistance. J Clin Microbiol 2001; 39: 2961-2963.
16. Rowe F, Superti SV, Scheibe RM, Dias CG. Agar diffusion, agar dilution, Etest, and agar screening test in the detection of methicillin resistance in staphylococci. Diagn Microbiol Infect Dis 2002; 43: 45-48.
17. National Committee for Clinical Laboratory Standards. Performance standards for antimicrobial susceptibility testing. M100-S10. Wayne, PA: NCCLS, 2001.
18. Bergan T, Bruun JN, Digranes A, Lingaas E, Melby KK, Sander J. Susceptibility testing of bacteria and fungi. Report from 'The Norwegian Working Group on Antibiotics'. Scand J Infect Dis 1997; 103(suppl): S1-S36.
19. Huang MB, Baker CN, Banerjee S, Tenover FC. Accuracy of the E test for determining antimicrobial susceptibilities of staphylococci, enterococci, Campylobacter jejuni, and Gram-negative bacteria resistant to antimicrobial agents. J Clin Microbiol 1992; 30: 3243-3248.
20. Frebourg NB, Nouet D, Lemée L, Martin E, Lemeland JF. Comparison of ATB Staph, Rapid ATB Staph, Vitek, and E-test methods for detection of oxacillin heteroresistance in staphylococci possessing mecA. J Clin Microbiol 1998; 36: 52-57.