The intracellular free amino acid pool represents tracer precursor enrichment for calculation of protein synthesis in cultured fibroblasts and myocytes

Journal of Nutrition

Volumn 134, Issue 6, 2004, Pages 1546-1550

  Martini, Wenjun Z ^b   Chinkes, David L ^a   Wolfe, Robert R ^a  

^a University of Texas Medical Branch School of Medicine   (United States)

^b US ARMY INSTITUTE OF SURGICAL RESEARCH   (United States)

Author keywords

GC MS; Plateau enrichment; Precursor enrichment; Protein synthesis; Stable isotopes

Indexed keywords

AMINO ACID; GLYCINE; ISOTOPE; NITROGEN 15; PHENYLALANINE; PROLINE; TRACER;

ARTICLE; CALCULATION; CELL CULTURE; CELL GROWTH; CHEMICAL REACTION KINETICS; CONTROLLED STUDY; CULTURE MEDIUM; FIBROBLAST; GAS CHROMATOGRAPHY; HUMAN; HUMAN CELL; MASS SPECTROMETRY; MUSCLE CELL; PROTEIN SYNTHESIS;

EID: 2642555594     PISSN: 00223166     EISSN: None     Source Type: Journal    
DOI: 10.1093/jn/134.6.1546     Document Type: Article

References (15)

1. Wolfe, R. R. (1998) Radioactive And Stable Isotope Tracers in Biomedicine: Principles and Practice of Kinetics Analysis. New York: Wiley-Liss.
2. Carraro, F., Hartl, W. H., Stuart, C. A., Layman, D. K., Jahoor, F. & Wolfe, R. R. (1990) Effect of exercise and recovery on muscle protein synthesis in human subjects. Am. J. Physiol. 258: E821-E831.
3. Reeds, P. J., Hackey, D. L., Patterson, B. W., Motil, K. J. & Klein, P. D. (1992) VLDL apolipoprotein B-100, a potential indicator of the isotopic labeling of the hepatic protein synthetic precursor pool in humans: studies with multiple stable isotopically labeled amino acids. J. Nutr. 122: 457-466.
4. Baumann, P. Q., Stirewalt, W. S., O'Rourke, B. D., Howard, D. & Nair, K. S. (1994) Precursor pools for protein synthesis: a stable isotope study in a swine model. Am. J. Physiol. 267: E203-E209.
5. El-Harake, W. A., Furman, M. A., Cook, B., Nair, K. S., Kukowski, J. & Brodsky, I. G. (1998) Measurement of dermal collagen synthesis rate in vivo in humans. Am. J. Physiol. 274: E586-E591.
6. Everett, A. W., Prior, G. & Zak, R. (1981) Equilibration of leucine between the plasma compartment and leucyl-tRNA in the heart, and turnover of cardiac myosin heavy chain. Biochem. J. 194: 365-368.
7. Martin, A. F., Rabinowitz, M., Blough, R., Prior, G. & Zak, R. (1977) Measurements of half-life of rat cardiac myosin heavy chain with leucyl-tRNA used as precursor pool. J. Biol. Chem. 252: 3422-3429.
8. McKee, E. E., Cheung, J. Y., Rannels, D. E. & Morgan, H. E. (1978) Measurement of the rate of protein synthesis and compartmentation of heart phenylalanine. J. Biol. Chem. 253: 1030-1040.
9. 13C-leucine. Clin. Sci. 77: 329-336.
10. Smith, K., Reynolds, N., Downie, S., Patel, A. & Rennie, M. J. (1998) Effects of flooding amino acids on incorporation of labeled amino acids into human muscle protein. Am. J. Physiol. 275: E73-E78.
11. Zhang, X. J., Chinkes, D. L., Sakurai, Y. & Wolfe, R. R. (1996) An isotopic method for measurement of muscle protein fractional breakdown rate in vivo. Am. J. Physiol. 270: E759-E767.
12. Airhart, J., Arnold, J. A., Stirewalt, W. S. & Low, R. B. (1982) Insulin stimulation of protein synthesis in cultured skeletal and cardiac muscle cells. Am. J. Physiol. 243: C91-C96.
13. Stirewalt, W. S. & Low, R. B. (1983) Effects of insulin in vitro on protein turnover in rat epitrochlearis muscle. Biochem. J. 210: 323-330.
14. Schneible, P. A., Airhart, J. & Low, R. B. (1981) Differential compartmentation of leucine for oxidation and for protein synthesis in cultured skeletal muscle. J. Biol. Chem. 256: 4888-4894.
15. Rennie, M. J., Smith, K. & Watt, P. W. (1994) Measurement of human tissue protein synthesis: an optimal approach Am. J. Physiol. 266: E298-E307.