Measurement of dermal collagen synthesis rate in vivo in humans

American Journal of Physiology - Endocrinology and Metabolism

Volumn 274, Issue 4 37-4, 1998, Pages

  El Harake, Wassim A ^a,c   Furman, Mikhail A ^a   Cook, Brian ^a,d   Nair, K Sreekumaran ^b   Kukowski, Jayme ^a   Brodsky, Irwin G ^a  

^a UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

^b MAYO CLINIC   (United States)

^c Jones Clinic   (United States)

^d NORTHWESTERN UNIVERSITY   (United States)

Author keywords

Connective tissue; Metabolism; Protein synthesis; Skin

Indexed keywords

ALBUMIN; AMINO ACID; AMINOACYL TRANSFER RNA; CARBON 13; COLLAGEN; CONTRACTILE PROTEIN; FIBRINOGEN; HYDROXYPROLINE; PROLINE; PROLINE TRANSFER RNA;

ARTICLE; COLLAGEN SYNTHESIS; CONTROLLED STUDY; FEMALE; HUMAN; HUMAN EXPERIMENT; HUMAN TISSUE; IN VIVO STUDY; MALE; PRIORITY JOURNAL; SKIN; TISSUE WATER;

EID: 0031957558     PISSN: 01931849     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpendo.1998.274.4.e586     Document Type: Article

References (24)

1. Abumrad, N. N., D. Rabin, M. P. Diamond, and W. W. Lacy. Use of heated superficial hand vein as an alternative site for the measurement of amino acid concentrations and for the study of glucose and alanine kinetics in man. Metabolism 30: 936-940, 1981.
2. 13C]leucine enrichment in human muscle proteins. Anal. Biochem. 239: 77-85, 1996.
3. Balagopal, P., O. E. Rooyackers, D. B. Adey, P. A. Ades, and K. S. Nair. Effects of aging on in vivo synthesis of skeletal muscle myosin heavy-chain and sarcoplasmic protein in humans. Am. J. Physiol. 273 (Endocrinol. Metab. 36): E790-E800, 1997.
4. Bradley, K., S. McConnell-Breul, and R. G. Crystal. Collagen in the human lung. J. Clin. Invest. 55: 543-550, 1975.
5. Brodsky, I. G., and J. T. Devlin. Effects of dietary protein restriction on regional amino acid metabolism in insulin-dependent diabetes mellitus. Am. J. Physiol. 270 (Endocrinol. Metab. 33): E148-E157, 1996.
6. Chomczynski, P., and N. Sacchi. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162: 156-159, 1987.
7. De Feo, P., M. G. Gaisano, and M. W. Haymond. Differential effects of insulin deficiency on albumin and fibrinogen synthesis in humans. J. Clin. Invest. 88: 833-840, 1991.
8. Foucault, A., and M. Caude. Ligand-exchange chromatography of enantiomeric amino acids on copper-loaded chiral bonded silica gel and of amino acids on copper(II)-modified silica gel. J. Chromatogr. A 185: 345-360, 1979.
9. Galambos, J. T., M. A. Hollingsworth, Jr., A. Falek, and W. D. Warren. The rate of synthesis of glycosaminoglycans and collagen by fibroblasts cultured from adult human liver biopsies. J. Clin. Invest. 60: 107-114, 1977.
10. Hames, B. D. Gel Electrophoresis of Proteins: A Practical Approach, edited by B. D. Hames and D. Rickwood. London: IRL, 1981.
11. Hildebran, J. N., J. Airchart, W. S. Stirewalt, and R. B. Low. Prolyl-tRNA-based rates of protein and collagen synthesis in human lung fibroblasts. Biochem. J. 198: 249-258, 1981.
12. Kehrer, J. P., Y. C. Lee, and S. M. Solem. Comparison of in vitro and in vivo rates of collagen synthesis in normal and damaged lung tissue. Exp. Lung Res. 10: 187-201, 1986.
13. Keiser, H. R., and A. Sjoerdsma. Direct measurement of the rate of collagen synthesis in skin. Clin. Chim. Acta 23: 341-346, 1969.
14. 3H]proline. Biochem. J. 206: 535-544, 1982.
15. Ljungqvist, O. H., M. Persson, G. C. Ford, and K. S. Nair. Functional heterogeneity of leucine pools in human skeletal muscle. Am. J. Physiol. 273 (Endocrinol. Metab. 36): E564-E570, 1997.
16. Mawhinney, T. P., R. S. R. Robinett, A. Atalay, and M. A. Madson. Analysis of amino acids as their tert-butyldimethylsilyl derivatives by gas-liquid chromatography and mass spectrometry. J. Chromatogr. A 358: 231-242, 1986.
17. 2 labelling. Biochem. J. 240: 431-435, 1986.
18. Opsahl, W. P., D. J. Deluca, and L. A. Ehrhart. Accelerated rates of collagen synthesis in atherosclerotic arteries quantified in vivo. Arteriosclerosis 7: 470-476, 1987.
19. Opsahl, W. P., and L. A. Ehrhart. Compartmentalization of proline pools and apparent rates of collagen and non-collagen protein synthesis in arterial smooth muscle cells in culture. Biochem. J. 243: 137-144, 1987.
20. Paulson, J. C., and F. E. Deatherage. Conditions for rapid hydrolysis of proteins by Dowex 50 catalysis. J. Am. Chem. Soc. 76: 6198-6200, 1954.
21. Scrimgeour, C. M., S. Downie, P. K. Rickuss, and M. J. Rennie. Collagen synthesis in human bone measured using stable isotope-labelled alanine and proline (Abstract). Proc. Nutr. Soc. 52: 258A, 1993.
22. Sodek, J. A comparison of the rates of synthesis and turnover of collagen and noncollagen proteins in adult rat periodontal tissues and skin using a microassay. Arch. Oral Biol. 22: 655-665, 1977.
23. Stirewalt, W. S., J. T. Coflesky, L. H. Young, and J. N. Evans. Protein synthesis in pulmonary arteries from rats exposed to hyperoxia. Am. J. Physiol. 264 (Lung Cell. Mol. Physiol. 8): L74-L79, 1993.
24. Toffolo, G., D. M. Foster, and C. Cobelli. Estimation of protein fractional synthetic rate from tracer data. Am. J. Physiol. 264 (Endocrinol. Metab. 27): E128-E135, 1993.