Ultrastructure of the osteocyte process and its pericellular matrix

Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology

Volumn 278, Issue 2, 2004, Pages 505-513

  You, Li Dan ^a   Weinbaum, Sheldon ^a   Cowin, Stephen C ^a   Schaffler, Mitchell B ^b  

^a City University of New York   (United States)

^b MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

Actin bundle; Osteocyte; Pericellular matrix; Pericellular space; Transverse element

Indexed keywords

ACTIN FILAMENT; ANIMAL CELL; ARTICLE; BONE; CELL ULTRASTRUCTURE; ELECTRON MICROSCOPY; EXTRACELLULAR MATRIX; FEMALE; HUMAN; HUMAN TISSUE; HUMERUS; MICROFILAMENT; MOUSE; NEWBORN; NONHUMAN; OSTEOCYTE; PERICELLULAR MATRIX; PRIORITY JOURNAL; TIBIA;

EID: 2642539994     PISSN: 0003276X     EISSN: None     Source Type: Journal    
DOI: 10.1002/ar.a.20050     Document Type: Article

References (45)

1. Bentolila V, Boyce TM, Fyhrie DP, Drumb R, Skerry TM, Schaffler MB. 1998. Intracortical remodeling in adult rat long bones after fatigue loading. Bone 23:275-281.
2. Cooper RR, Milgram JW, Robinson RA. 1966. Morphology of the osteon. An electron microscopic study. J Bone Joint Surg Am 48:1239-1271.
3. Cowin SC, Weinbaum S, Zeng Y. 1995. A case for bone canaliculi as the anatomical site of strain generated potentials. J Biomech 28:1281-1297.
4. Fritton SP, McLeod KJ, Rubin CT. 2000. Quantifying the strain history of bone: spatial uniformity and self-similarity of low-magnitude strains. J Biomech 33:317-325.
5. Hascall VC. 2000. Hyaluronan, a common thread. Glycoconjugate J 17:607-616.
6. Holtrop ME. 1975. The ultrastructure of bone. Ann Clin Lab Sci 5:264-271.
7. Hughes DE, Salter DM, Simpson R. 1994. CD44 expression in human bone: a novel marker of osteocytic differentiation. J Bone Miner Res 9:39-44.
8. Hunziker EB, Herrmann W, Schenk RK. 1982. Improved cartilage fixation by ruthenium hexammine trichloride (RHT). A prerequisite for morphometry in growth cartilage. J Ultrastruct Res 81:1-12.
9. Jamal HH, Aubin JE. 1996. CD44 expression in fetal rat bone: in vivo and in vitro analysis. Exp Cell Res 223:467-477.
10. King GJ, Holtrop ME. 1975. Actin-like filaments in bone cells of cultured mouse calvaria as demonstrated by binding to heavy meromyosin. J Cell Biol 66:445-451.
11. Knothe Tate ML, Knothe U, Niederer P. 1998. Experimental elucidation of mechanical load-induced fluid flow and its potential role in bone metabolism and functional adaptation. Am J Med Sci 316:189-195.
12. Knothe Tate ML, Knothe U. 2000. An ex vivo model to study transport processes and fluid flow in loaded bone. J Biomech 33:247-254.
13. Knothe Tate ML, Tami A, Nasser P, Steck R, Schaffler M. 2001. Permeability characteristics of different molecular tracers in loaded and unloaded bone. In: Proceedings of the 47th Annual Meeting of the Orthopaedic Research Society, San Francisco. p 0138.
14. Kufahl RH, Saha S. 1990. A theoretical model for stress-generated fluid flow in the canaliculi-lacunae network in bone tissue. J Biomech 23:171-180.
15. Lodish H, Berk A, Zipursky L, Matsudaira P, Baltimore D, Darnell J. 1999. Molecular cell biology. 4th ed. New York: W.H. Freeman and Company.
16. Marotti G, Delrio N, Marotti F, Fadda M. 1979. Quantitative analysis of the bone destroying activity of osteocytes and osteoclasts in experimental disuse osteoporosis. Ital J Orthop Traumatol 5:225-240.
17. Marotti G. 1990. Ultrastructure of skeletal tissue. Boston: Kluwer Academic Publisher.
18. Marotti G, Cane V, Palazzini S, Palumbo C. 1990. Structure-function relationships in the osteocyte. Ital J Miner Electrolyte Metab 4:93-106.
19. Marotti G. 1996. The structure of bone tissues and the cellular control of their deposition. Ital J Anat Embryol 101:25-79.
20. McDonald J, Hascall VC. 2002. Hyaluronan minireview series. J Biol Chem 15;277:4575-4579.
21. Mooseker MS, Tilney LG. 1975. Organization of an actin filament-membrane complex. Filament polarity and membrane attachment in the microvilli of intestinal epithelial cells. J Cell Biol 67:725-743.
22. Nakamura H, Kenmotsu S, Sakai H, Ozawa H. 1995. Localization of CD44, the hyaluronate receptor, on the plasma membrane of osteocytes and osteoclasts in rat tibiae. Cell Tissue Res 280:225-233.
23. Nakamura H, Ozawa H. 1996. Immunolocalization of CD44 and the ERM family in bone cells of mouse tibiae. J Bone Miner Res 11:1715-1722.
24. Noonan KJ, Stevens JW, Tammi R, Tammi M, Hernandez JA, Midura RJ. 1996. Spatial distribution of CD44 and hyaluronan in the proximal tibia of the growing rat. J Orthop Res 14:573-581.
25. Palumbo C. 1986. A three-dimensional ultrastructural study of osteoid-osteocytes in the tibia of chick embryos. Cell Tissue Res 246:125-131.
26. Palumbo C, Palazzini S, Marotti G. 1990a. Morphological study of intercellular junctions during osteocyte differentiation. Bone 11:401-406.
27. Palumbo C, Palazzini S, Zaffe D, Marotti G. 1990b. Osteocyte differentiation in the tibia of newborn rabbit: an ultrastructural study of the formation of cytoplasmic processes. Acta Anat 137:350-358.
28. Piekarski K, Munro M. 1977. Transport mechanism operating between blood supply and osteocytes in long bones. Nature 269:80-82.
29. Rubin CT, Lanyon LE. 1984. Regulation of bone formation by applied dynamic loads. J Bone Joint Surg Am 66:397-402.
30. Salzstein RA, Pollack SR, Mak AF, Petrov N. 1987. Electromechanical potentials in cortical bone - I. A continuum approach. J Biomech 20:261-270.
31. Sauren YM, Mieremet RH, Groot CG, Scherft JP. 1992. An electron microscopic study on the presence of proteoglycans in the mineralized matrix of rat and human compact lamellar bone. Anat Rec 232:36-44.
32. Shapiro F, Cahill C, Malatantis G, Nayak RC. 1995. Transmission electron microscopic demonstration of vimentin in rat osteoblast and osteocyte cell bodies and processes using the immunogold technique. Anat Rec 241:39-48.
33. Steflik DE, Sisk AL, Parr GR, Lake FT, Hanes PJ, Berkery DJ, Brewer P. 1994. Transmission electron and high-voltage electron microscopy of osteocyte cellular processes extending to the dental implant surface. J Biomed Mater Res 28:1095-1107.
34. Tanaka-Kamioka K, Kamioka H, Ris H, Lim SS. 1998. Osteocyte shape is dependent on actin filaments and osteocyte processes are unique actin-rich projections. J Bone Miner Res 13:1555-1568.
35. Wang L, Fritton SP, Cowin SC, Weinbaum S. 1999. Fluid pressure relaxation depends upon osteonal microstructure: modeling an oscillatory bending experiment. J Biomech 32:663-672.
36. Wang L, Cowin SC, Weinbaum S, Fritton SP. 2000. Modeling tracer transport in an osteon under cyclic loading. Ann Biomed Eng 28:1200-1209.
37. Wang L, Ciani C, Doty SB, Fritton SP. 2004. Delineating bone's interstitial fluid pathway in vivo. Bone 34:499-509.
38. Wassermann F, Yaeger J. 1965. Fine structure of osteocyte capsule and of wall of lacunae in bone. Z Zellforsch Mikrosk Anat 67:636-652.
39. Weibel ER. 1980. Stereological methods. New York: Academic Press.
40. Weinbaum S, Cowin SC, Zeng Y. 1994. A model for the excitation of osteocytes by mechanical loading-induced bone fluid shear stresses. J Biomech 27:339-360.
41. Weinbaum S, Guo P, You L. 2001. A new view of mechanotransduction and strain amplification in cells with microvilli and cell processes. Biorheology 38:119-142.
42. Weinger JM, Holtrop ME. 1974. An ultrastructural study of bone cells: the occurrence of microtubules, microfilaments and tight junctions. Calcif Tissue Res 14:15-29.
43. You J, Yellowley CE, Donahue HJ, Zhang Y, Chen Q, Jacobs CR. 2000. Substrate deformation levels associated with routine physical activity are less stimulatory to bone cells relative to loading-induced oscillatory fluid flow. J Biomech Eng 122:387-393.
44. You L, Cowin SC, Schaffler MB, Weinbaum S. 2001. A model for strain amplification in the actin cytoskeleton of osteocytes due to fluid drag on pericellular matrix. J Biomech 34:1375-1386.
45. Zeng Y, Cowin SC, Weinbaum S. 1994. A fiber matrix model for fluid flow and streaming potentials in the canaliculi of an osteon. Ann Biomed Eng 22:280-292.