Cysteine but not glutathione modulates the radiosensitivity of human melanoma cells by affecting both survival and DNA damage

Pigment Cell Research

Volumn 17, Issue 3, 2004, Pages 275-280

  Kinnaert, E ^a   Duez, P ^b   Morandini, R ^a   Dubois, J ^b   Van Houtte, P ^a   Ghanem, G ^a  

^a JULES BORDET INSTITUTE   (Belgium)

^b UNIVERSITÉ LIBRE DE BRUXELLES   (Belgium)

Author keywords

Cysteine; DNA damage; Glutathione; Human melanoma; Ionizing radiation; Radiotoxicity

Indexed keywords

BUTHIONINE SULFOXIMINE; CYSTEINE; DNA; ENZYME INHIBITOR; GLUTAMATE CYSTEINE LIGASE; GLUTATHIONE; MELANIN;

ARTICLE; CELL SURVIVAL; COMET ASSAY; CONTROLLED STUDY; CYTOTOXICITY; DNA DAMAGE; HUMAN; HUMAN CELL; IONIZING RADIATION; MELANOMA; MELANOMA CELL; OXIDATIVE STRESS; RADIATION DOSE; RADIOSENSITIVITY;

BUTHIONINE SULFOXIMINE; CELL LINE, TUMOR; CELL SURVIVAL; COMET ASSAY; CYSTEINE; DNA DAMAGE; DOSE-RESPONSE RELATIONSHIP, RADIATION; GLUTATHIONE; HUMANS; MELANOMA; PIGMENTATION; RADIATION TOLERANCE; RADIATION-SENSITIZING AGENTS;

EID: 2642538300     PISSN: 08935785     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1600-0749.2004.00146.x     Document Type: Article

References (30)

1. Biaglow JE, Varnes ME, Epp ER, Clark EP, Astor M. Factors involved in depletion of glutathione from A549 human lung carcinoma cells: implications for radiotherapy. Int J Rad Oncol Biol Phys 1984;10:1221-1227
2. Mullineau PM, Creissen GP. Glutathione reductase: regulation and role in oxidative stress. In: Scandalios JG, eds. Oxidative Stress and Molecular Biology of Antioxidant Defenses. New York: Cold Spring Harbor Laboratory Press; 1997. pp. 667-713
3. Kosower NS, Kosower EM. The glutathione status of cells. Int Rev Cytol 1978;54:124-138
4. Bump ED, Martin Brown J. Role of glutathione in the radiation response of mammalian cells in vitro and in vivo. Pharmac Ther 1990;47:117-136
5. Munday R, Winterbourn CC. Reduced glutathione in combination with superoxide dismutase as an important biological antioxidant defence mechanism. Biochem Pharmacol 1989;38:4349-4352
6. Riley PA. Molecules in focus = melanin. Int J Biochem Cell Biol 1997;29:1235-1239
7. Meyskens Jr FL, Farmer P, Fruehauf J. Redox regulation in human melanocytes and melanoma. Pigment Cell Res 2001;14:148-154
8. Hill HZ, Cathcart KN, Bargellini J, Trizna Z, Hill GJ, Schallreuter KU, Wood JM. Does melanin affect the low LET radiation response of Cloudman S91 mouse melanoma cell lines? Pigment Cell Res 1991;4:80-86
9. Kinnaert E, Morandini R, Simon S, Hill HZ, Ghanem G, Van Houtte P. The degree of pigmentation modulates the radiosensitivity of human melanoma cells. Rad Res 2000;154:497-502
10. del Marmol V, Solano F, Sels A, Huez G, Libert A, Lejeune F, Ghanem G. Glutathione depletion increase tyrosinase activity in human melanoma cells. J Invest Dermatol 1993;101:871-874
11. Griffith OW. Determination of glutathione disulfide using glutathione reductase and 2-vinylpyridine. Anal Biochem 1980;106:207-212
12. Ito S, Fujita K. Microanalysis of eumelanin and pheomelanin in hair and melanomas by chemical degradation and liquid chromatography. Anal Biochem 1985;144:527-536
13. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-254
14. DeBoek M, Touil N, DeVisscher G, Aka Vande P, Kirsh-Volders M. Validation and implementation of an internal standard in comet assay analysis. Mutation Res 2000;469:181-197
15. Thames HD, Hendry JH eds. Repair Kinetics. In: Fractionation in Radiotherapy. London, New York, Philadelphia: Taylor & Francis; 1987. p. 89
16. del Marmol V, Ito S, Bouchard B, Libert A, Wakamatsu K, Ghanem G, Solano F. Cysteine deprivation promotes eumelanogenesis in human melanoma cells. J Invest Dermatol 1996;107:698-702
17. Meister A, Anderson M. Glutathione. Ann Rev Biochem 1983;52:711-760
18. Jimenez-Cervantes C, Solano F, Kobayashi T, Urabe K, Hearing VJ, Lozano JA, Garcia-Borron JC. A new enzymatic function in the melanotic pathway the 5,6-dihydroxyindole-2-carboxylic acid oxydase activity of tyrosinase related protein-1 (TRP-1). J Biol Chem 1994;269:17993-18001
19. Guichard M, Lespinasse F, Malaise EP. Influence of buthionine sulfoxime and misonidazole on glutathione level and radiosensitivity of human xenografts. Rad Res 1986;105:115-125
20. Dethmers JK, Meister A. Glutathione export by human lymphoid cells: depletion of glutathione by inhibition of its synthesis decreases export and increase sensitivity to irradiation. Proc Natl Acad Sci USA 1981;78:7492-7496
21. Jevtovic-Todorovic V, Guenthner TM. Depletion of a discrete nuclear glutathione pol by oxidative stress, but not by buthionine sulfoximine. Correlation with enhanced alkylating agent cytotoxicity to human melanoma cells in vitro. Biochem Pharmacol 1992;44:1383-1393
22. Wolff GL. Regulation of yellow pigment formation in mice: a historical perspective. Pigment Cell Res 2003;16:2-15
23. Yu NY, Brown JM. Depletion of glutathione in vivo as a method of improving the therapeutic ratio of misonidazole and SR 2508. Int J Rad Oncol Biol Phys 1984;10:1265-1269
24. Prezioso JA, Schields D, Wang N, Rosenstein M. Role of γ-glutamyltranspeptidase-mediated glutathione transport on the radiosensitivity of B16 melanoma variant cell lines. Int J Rad Oncol Biol Phys 1994;30:373-381
25. Rozanowska M, Sarna T, Land EJ, Truscott TG. Free radical scavenging properties of melanin interaction of eu- and pheomelanin models with reducing and oxidising radicals. Free Rad Biol Med 1999;26:518-525
26. Mileo AM, Mattei E, Fanuele M, Delpino A, Ferrini U. Differential radiosensitivity in cultured B-16 cells following interrupted melanogenesis induced by glucosamine. Pigment Cell Res 1989;2:167-170
27. Applegate LA, Scaletta C, Labidi F, Vile GF, Frenk E. Suseceptibility of human melanoma cells to oxidative stress including UVA radiation. Int J Cancer 1996;67:430-434
28. Scalia M, Geremia E, Corsaro C, Santoro C, Baratta D, Sichel G. Lipid peroxidation in pigmented and unpigmented liver tissues: protective role of melanin. Pigment Cell Res 1990;3:115-119
29. Sarna T, Swartz M. The physical properties of melanins. In: Nordlund JJ, Boissy RE, Hearing VJ, King RA, Ortonne JP, eds. The Pigmentary System Physiology and Physiopathology. New York: Oxford University Press; 1998. pp. 333-358
30. Inazu M, Mishima Y. Detection of melanogenic and pheomelanogenetic melanosomes in the same normal melanocyte. J Invest Dermatol 1993;100:172s-175s