Separation of jack bean (Canavalia ensiformis) urease isoforms by immobilized metal affinity chromatography and characterization of insecticidal properties unrelated to ureolytic activity

Plant Science

Volumn 167, Issue 2, 2004, Pages 241-246

  Follmer, Cristian ^a   Wassermann, German E ^a   Carlini, Célia R ^a  

^a FEDERAL UNIVERSITY OF RIO GRANDE DO SUL   (Brazil)

Author keywords

Canatoxin; Canavalia ensiformis; IMAC; Insecticide; Nickel; Urease; Zinc

Indexed keywords

ENZYMES; INSECT CONTROL; MONOMERS; SEED;

JACK BEAN SEEDS; JACK BEAN UREASE (JBU);

PLANTS (BOTANY);

CANAVALIA; CANAVALIA ENSIFORMIS; DYSDERCUS PERUVIANUS; GOSSYPIUM HIRSUTUM; HEMIPTERA; HEXAPODA; INSECTA; PHASEOLUS (ANGIOSPERM);

EID: 2642536959     PISSN: 01689452     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.plantsci.2004.03.019     Document Type: Article

References (30)

1. Dixon N.E., Gazzola C., Blakeley R.L., Zerner B. Jack bean urease (EC 3.5.1.5). A metalloenzyme. A simple biological role for nickel. J. Am. Chem. Soc. 97:1975;4131-4133
2. Mobley H.L., Hausinger R.P. Microbial ureases: significance, regulation and molecular characterization. Microbiol. Rev. 53:1989;85-108
3. Sumner J.B. The isolation and crystallization of the enzyme urease. J. Biol. Chem. 69:1926;435-441
4. Hogan M.E., Swift I.E., Done J. Urease assay and ammonia release from leaf tissues. Phytochemistry. 22:1983;663-667
5. Polacco J.C., Sparks R.B. Patterns of urease synthesis in developing soybeans. Plant Physiol. 70:1982;189-194
6. Polacco J.C., Winkler R.G. Soybean leaf urease: a seed enzyme. Plant Physiol. 74:1984;800-803
7. Polacco J.C., Havir E.A. Comparisons of soybean urease isolated from seed and tissue culture. J. Biol. Chem. 254:1979;1707-1715
8. Polacco J.C., Krueger R.W., Winkler R.G. Structure and possible ureide degrading function of the ubiquitous urease of soybean. Plant Physiol. 79:1985;794-800
9. Polacco J.C., Holland M.A. Roles of urease in plant cells. Int. Rev. Cytol. 145:1993;65-103
10. Carlini C.R., Guimarães J.A. Isolation and characterization of a toxic protein from Canavalia ensiformis (jack bean) seeds, distinct from concanavalin A. Toxicon. 19:1981;667-675
11. Follmer C., Barcellos G.B.S., Zingali R.B., Machado O.L.T., Alves E.W., Barja-Fidalgo C., Guimarães J.A., Carlini C.R. Canatoxin, a toxic protein from jack beans (Canavalia ensiformis), is a variant form of urease (EC 3.5.1.5): biological effects of urease independent of its ureolytic activity. Biochem. J. 360:2001;217-224
12. Carlini C.R., Guimarães J.A., Ribeiro J.M. Platelet release reaction and aggregation induced by canatoxin, a convulsant protein: evidence for the involvement of the platelet lipoxygenase pathway. Br. J. Pharmacol. 84:1985;551-560
13. Ghazaleh F.A., Francischetti I.M., Gombarovits M.E., Carlini C.R. Stimulation of calcium influx and platelet activation by canatoxin: methoxyverapamil inhibition and downregulation by cGMP. Arch. Biochem. Biophys. 339:1997;362-367
14. Carlini C.R., Guimaraes J.A. Plant and microbial toxic proteins as hemilectins: emphasis on canatoxin. Toxicon. 29:1991;791-806
15. Carlini C.R., Oliveira A.E., Azambuja P., Xavier-Filho J., Wells M.A. Biological effects of canatoxin in different insect models: evidence for a proteolytic activation of the toxin by insect cathepsinlike enzymes. J. Econ. Entomol. 90:1997;340-348
16. Ferreira-DaSilva C.T., Gombarovits M.E., Masuda H., Oliveira C.M., Carlini C.R. Proteolytic activation of canatoxin, a plant toxic protein, by insect cathepsin-like enzymes. Arch. Insect Biochem. Physiol. 44:2000;162-171
17. Carlini C.R., Grossi-de-Sá M.F. Plant toxic proteins with insecticidal properties. A review on their potentialities as bioinsecticides. Toxicon. 40:2002;1515-1539
18. Blakeley R.L., Webb E.C., Zerner B. Jack bean urease (EC 3.5.1.5). A new purification and reliable rate assay. Biochemistry. 8:1969;1984-1990
19. Jabri E., Lee M.H., Hausinger R.P., Karplus P.A. Preliminary crystallographic studies of urease from jack bean and from Klebsiella aerogenes. J. Mol. Biol. 227:1992;934-937
20. Porath J. Immobilized metal ion affinity chromatography. Protein Exp. Purif. 3:1992;263-281
21. Spector T. Refinement of the Coomassie Blue method of protein quantitation. A simple and linear spectrophotometric assay for 0.5 to 50 μg of protein. Anal. Biochem. 86:1978;142-146
22. Weatherburn M.W. Phenol-hypochlorite reaction for determination of ammonia. Anal. Chem. 39:1967;971-974
23. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate polyacrylamide gel electrophoresis. J. Biol. Chem. 244:1969;4406-4412
24. Malmqvist K.G. Quantitative PIXE analysis of biomedical material - sample preparation. Nucl. Instrum. Meth. Phys. Res. 49B:1990;183-190
25. Mangelson N.F., Hill M.W., Nielson K.K., Ryder J.F. Proton induced X-ray emission analysis of biological samples: some approaches and applications. Nucl. Instrum. Meth. Phys. Res. 142:1977;133-142
26. Campbell J.L., Hopman T.L., Maxwell J.A., Nejedly J. The Guelph PIXE software package III: alternative proton database. Nucl. Instrum. Meth. Phys. Res. 170B:2000;193-204
27. Hu S., Dovichi N.J. Capillary electrophoresis for the analysis of biopolymers. Anal Chem. 74:2002;2833-2850
28. Mamiya G., Takishima K., Masakuni M., Kayumi T., Ogawa K., Sekita T. Complete amino acid sequence of jack bean urease. Proc. Jpn. Acad. 61:1985;395-398
29. Pires-Alves M., Grossi-de-Sá F., S Barcellos G.B., Carlini C.R., Moraes M.G. Characterization and expression of a novel member (JBURE-II) of the urease gene family from jackbean (Canavalia ensiformis DC). Plant Cell Physiol. 44:2003;139-145
30. Zerner B. Recent advances in the chemistry of an old enzyme. Urease. Bioorg. Chem. 19:1991;116-131