Proteolytic activation of canatoxin, a plant toxic protein, by insect cathepsin-like enzymes

Archives of Insect Biochemistry and Physiology

Volumn 44, Issue 4, 2000, Pages 162-171

  Ferreira DaSilva, Cláudio T ^a   Gombarovits, Maria Emília C ^b   Masuda, Hatisaburo ^c   Oliveira, Cátia M ^c   Carlini, Célia R ^a  

^a FEDERAL UNIVERSITY OF RIO GRANDE DO SUL   (Brazil)

^b INSTITUTO OSWALDO CRUZ   (Brazil)

^c FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

Author keywords

Activation; Callosobruchus maculatus; Canatoxin; Cathepsins; Insect; Rhodnius prolixus

Indexed keywords

BRUCHINAE; CALLOSOBRUCHUS MACULATUS; CANAVALIA; CANAVALIA ENSIFORMIS; HEXAPODA; INSECTA; RHODNIUS PROLIXUS;

CANATOXIN; CATHEPSIN; LECTIN; TOXIN; VEGETABLE PROTEIN;

ANIMAL; ARTICLE; BIOASSAY; LARVA; MALE; METABOLISM; RHODNIUS;

ANIMALS; BIOLOGICAL ASSAY; CATHEPSINS; LARVA; LECTINS; MALE; PLANT PROTEINS; RHODNIUS; TOXINS, BIOLOGICAL;

EID: 0034242674     PISSN: 07394462     EISSN: None     Source Type: Journal    
DOI: 10.1002/1520-6327(200008)44:4<162::aid-arch3>3.0.co;2-%23     Document Type: Article

References (29)

1. 2+-ATPaSe of sarcoplasmic reticulum membranes. Toxicon 30: 1411-1418.
2. Ansorge W. 1985. Fast and sensitive detection of protein and DNA bands by treatment with potassium permanganate. J Biochem Biophys Methods 11: 11-20.
3. Barcellos GBS. 1992. Canatoxin: structural analysis and comparative studies with concanavalin A in Canavalia ensiformis seeds. MS thesis. Universidade Federal do Rio de Janeiro, Brazil.
4. Barcellos GBS, Almeida LA, Moreira RA, Sousa-Cavada B, Oliveira JTA, Carlini CR. 1993. Canatoxin-, concanavalin A-, and canavalin-cross reactive materials during maturation of Canavalia brasiliensis seeds. Planta 18: 397-402.
5. Barja-Fidalgo C, Guimarães JA, Carlini CR. 1991. Canatoxin, a plant protein, induces insulin secretion from isolated pancreatic islets. Endocrinology 128:675-679.
6. Campos FAP, Xavier-Filho J, Silva CP, Ary MB. 1989. Resolution and partial characterization of proteinases and alpha-amylases from midguts of larvae of the bruchid beetle Callosobruchus maculatus (F). Comp Biochem Physiol 92B:51-57.
7. Carlini CR, Guimarães JA. 1981. Isolation and characterization of a toxic protein from Canavalia ensiformis (jack bean) seeds, distinct from concanavalin A. Toxicon 19:667-676.
8. Carlini CR, Guimarães JA. 1991. Plant and microbial toxic proteins as hemilectins: emphasis on canatoxin. Toxicon 29:791-806.
9. Carlini CR, Guimarães JA, Ribeiro JMC. 1985. Platelet release reaction and aggregation induced by canatoxin, a convulsant protein: evidences for the involvement of the platelet lipoxygenase pathway. Br J Pharmacol 84:551-560.
10. Carlini CR, Barcellos GBS, Baeta-Neves ADV, Guimarães JA. 1988. Immunoreactivity for canatoxin and concanavalin A among proteins from leguminous seeds. Phytochemistry 27:25-30.
11. Carlini CR, Oliveira AE, Azambuja P, Xavier-Filho J, Wells MA. 1997. Biological effects of canatoxin in different insect models. Evidence for a proteolytic activation of the toxin by insect cathepsinlike enzymes. J Econ Entomol. 90:340-348.
12. Choma CT, Surewicz WK, Carey PR, Pozsgay M, Raynor T, Kaplan H. 1990. Unusual proteolysis of the protoxin and toxin from Bacillus thuringiensis. Structural implications. Eur J Biochem. 189:523-527.
13. Fast PG, Videnova E. 1974. The delta-endotoxin of Bacillus thuringiensis. V. On the occurrence of endotoxin fragments in hemolymph. J Invertebr Pathol 23: 280-284.
14. Furtado AF, Regis LN, Falcao PH, Sewell HF, Azambuja P, Garcia ES. 1990. Monoclonal antibodies against brain neurosecretory A cells of Panstrongylus megistus inhibit moulting and egg production in triatomines. Mem Inst Oswaldo Cruz 85:113-114.
15. Garcia ES, Macarini JD, Garcia MLM. 1973. The cycle of intestinal protease activity in larval and adult Rhodnius prolixus. Rev Bras Biol. 33:314-320.
16. Garcia ES, Garcia MLM, Guimarães JA. 1981. Pepstatin inhibition of molting and oviposition in Rhodnius prolixus (Hemiptera: Reduviidae). J Med Entomol 18: 441-445.
17. Garcia ES, Azambuja P, Contreras VT. 1984. Large-scale rearing of Rhodnius prolixus and production of metacyclic trypomastigotes of Trypanosoma cruzi. In: Morel CM, editor. Genes and antigens of parasites, a laboratory manual. Rio de Janeiro, Brazil: Oswaldo Cruz Foundation and World Health Organization, p. 44-47.
18. Ghazaleh FA, Francischetti IMB, Gombarovits MEC, Carlini CR. 1997. Stimulation of calcium influx and platelet activation by canatoxin: methoxy-verapamil inhibition and downregulation by cGMP. Arch Biochem Biophys 339:362-367.
19. Gondim KC, Coelho HS, Oliveira PL, Masuda H. 1989. Lipophorin from Rhodnius prolixus: purification and partial characterization. Insect Biochem 19:153-161.
20. Haider MZ, Knowles BH, Ellar DJ. 1986. Specificity of Bacillus thuringiensis var. colmeri insecticidal δ-endotoxin is determined by differential proteolytic processing of the protoxin by larval gut proteases. Eur J Biochem 156: 532-540.
21. Keller M, Sneh B, Strizhov N, Prudovsky E, Regev A, Koncz C, Schell J, Zilberstein A. 1996. Digestion of delta-endotoxin by gut proteases may explain reduced sensitivity of advanced instar larvae of Spodoptera littoralis to CryIC. Insect Biochem Mol Biol 26:365-373.
22. Knowles BH, Ellar DJ. 1987. Colloid-osmotic lysis is a general feature of the mechanism of action of Bacillus thuringiensis δ-endotoxins with different insect specificity. Biochim Biophys Acta 924:509-518.
23. Laemmli UK. 1970. Cleavage of structural proteins during assembly of the bacteriophage T4. Nature (Lond.) 227: 680-685.
24. Lemos FJA, Campos FAP, Silva CP, Xavier-Filho J. 1990. Proteinases and amylases of larval midgut of Zabrotes subfasciatus reared on cowpea (Vigna unguiculata) seeds. Entomol Exp Appl 56:219-227.
25. Martens JWM, Visser B, Vlak JM, Bosch D. 1995. Mapping and characterization of the entomocidal domain of the Bacillus thuringiensis CryIA(b) protoxin. Mol Gen Genet 247:482-487.
26. Ribeiro-DaSilva G, Prado JF. 1993. Increased insulin circulating levels induced by canatoxin in rats. Toxicon 31: 1131-1136.
27. Silva CP, Xavier-Filho J. 1991. Comparison between the levels of aspartic and cysteine proteinases of the larval midguts of Callosobruchus maculatus (F.) and Zabrotes subfasciatus (Boh.) (Coleoptera: Bruchidae). Comp Biochem Physiol 99B:529-533.
28. Terra WR, Ferreira C, Garcia ES. 1988. Origin, distribution, properties and functions of the major Rhodnius prolixus midgut hydrolases. Insect Biochem 18:423-434.
29. Vasconcelos IM, Trentin A, Guimarães JA, Carlini CR. 1994. Purification and physicochemical characterization of soyatoxin, a novel toxic protein isolated from soybean (Glycine max). Arch Biochem Biophys 312:357-366.