Real-time in-cell 19F NMR study on uptake of fluorescent and nonfluorescent 19F-octaarginines into human Jurkat cells

Chemistry Letters

Volumn 34, Issue 7, 2005, Pages 1064-1065

  Okamura, Emiko ^a   Ninomiya, Keiko ^a,b   Futaki, Shiroh ^a,b   Nagai, Yasuharu ^a   Kimura, Tomohiro ^a   Wakai, Chihiro ^a   Matubayasi, Nobuyuki ^a   Sugiura, Yukio ^a   Nakahara, Masaru ^a  

^a KYOTO UNIVERSITY   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 26044483853     PISSN: 03667022     EISSN: None     Source Type: Journal    
DOI: 10.1246/cl.2005.1064     Document Type: Article

References (15)

1. J. Zhang, R. E. Campbell, A. Y. Ting, and R. Y. Tsien, Nat. Rev. Mol. Cell Biol., 3, 906 (2002).
2. Octaarginine is a cationic membrane-permeable peptide facilitating intracellular delivery of exogeneous proteins. S. Futaki, T. Suzuki, W. Ohashi, T. Yagami, S. Tanaka, K. Ueda, and Y. Sugiura, J. Biol. Chem., 276, 5836 (2001).
3. a) E. Okamura and M. Nakahara, in "Liquid Interfaces in Chemical, Biological, and Pharmaceutical Applications," ed. by A.G. Volkov, Marcel Dekker, New York (2001), Chap. 32, p 775.
4. b) E. Okamura, C. Wakai, N. Matubayasi, Y. Sugiura, and M. Nakahara, Phys. Rev. Lett., 93, 248101 (2004).
5. a) Y. Xu, P. Tang, W. Zhang, L. Firestone, and P. M. Winter, Anesthesiology, 83, 766 (1995).
6. b) S.-P. Williams, P. M. Haggie, and K. M. Brindle, Biophys. J., 72, 490 (1997).
7. c) D. Bilecen, A.-C. Schulte, A. Kaspar, E. Kustermann, J. Seelig, D. v. Elverfeldt, and K. Scheffler, NMR Biomed., 16, 144 (2003).
8. Z. Serber and V. Dötsch, Biochemistry, 40, 14317 (2001).
9. 19F-octaarginine (in preparation).
10. The D-isomers are selected to avoid peptide fragmentation by the intracellular peptidase. All the peptides were prepared by Fmoc-solid-phase synthesis; see Supporting Information.
11. The digital resolution of the spectra was 0.2 Hz, corresponding to the uncertainty of the chemical shifts of ±0.0005 ppm.
12. 19F-octaarginine I.
13. a) J. E. Smotrys and M. E. Linder, Annu. Rev. Biochem., 73, 559 (2004).
14. b) T. Kimura, E. Okamura, N. Matubayasi, K. Asami, and M. Nakahara, Biophys. J., 87, 375 (2004).
15. N. Kahya, D. Scerfeld, K. Bacia, and P. Schwille, J. Struct. Biol., 147, 77 (2004).