Enhanced sensitivity and precision in an enzyme-linked immunosorbent assay with fluorogenic substrates compared with commonly used chromogenic substrates

Analytical Biochemistry

Volumn 345, Issue 2, 2005, Pages 227-236

  Meng, Yuan ^a   High, Katrina ^a   Antonello, Joseph ^a   Washabaugh, Michael W ^a   Zhao, Qinjian ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

Affinity constant determination; ELISA; Enzyme substrate; Fluorescence; Fluorogenic substrate; Immuno assay; Level of detection; Level of quantitation; Precision; Sensitivity; Z factor

Indexed keywords

CHROMOGENICS; ENZYMATIC HYDROLYSIS; FLUORESCENCE; SIGNAL TO NOISE RATIO;

AFFINITY CONSTANTS; ELISA; ENZYME SUBSTRATES; FLUOROGENIC SUBSTRATE; IMMUNO-ASSAY; LEVEL OF QUANTITATION; PRECISION; SENSITIVITY; Z-FACTOR;

SUBSTRATES;

10 ACETYL 3,7 DIHYDROXYPHENOXAZINE; 2 PHENYLENEDIAMINE; 2' (2 BENZOTHIAZOYL) 6' HYDROXYBENZOTHIAZOLE PHOSPHATE; 4 METHYLUMBELLIFERYL PHOSPHATE; AMPLEX RED; ATTOPHOS; BENZIDINE DERIVATIVE; CHROMOGENIC SUBSTRATE; ENZYME; FLUORINE DERIVATIVE; PHENOXAZINE DERIVATIVE; PHENYLENEDIAMINE DERIVATIVE; PHLORETIC ACID; PHOSPHATE; PROPIONIC ACID DERIVATIVE; QUANTABLU; SUPERRED; TETRAMETHYLBENZIDINE; UNCLASSIFIED DRUG;

ACCURACY; ARTICLE; BINDING AFFINITY; COMPARATIVE STUDY; CONCENTRATION (PARAMETERS); DRUG STRUCTURE; ENZYME LINKED IMMUNOSORBENT ASSAY; FLUORESCENCE; MONITORING; PARTICULATE MATTER; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SIGNAL NOISE RATIO;

EID: 25444513733     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2005.07.026     Document Type: Article

References (29)

1. J.R. Crowther, L. Angarita, and J. Anderson Evaluation of the use of chromogenic and fluorogenic substrates in solid-phase enzyme linked immunosorbent assay (ELISA) Biologicals 18 1990 331 336
2. Z. Huang, N.A. Olson, W. You, and R.P. Haugland A sensitive competitive ELISA for 2,4-dinitrophenol using 3,6-fluoresecein diphosphate as a fluorogenic substrate J. Immunol. Methods 149 1992 261 266
3. R.J. Cano, M.J. Torres, R.E. Klem, and J.C. Palomares DNA hybridization assay using AttoPhos, a fluorescent substrate for alkaline phosphatase BioTechniques 12 1992 264 268
4. I.M. Roberts, S.L. Jones, R.R. Premier, and J.C. Cox A comparison of the sensitivity and specificity of enzyme immunoassays and time-resolved fluoroimmunoassay J. Immunol. Methods 143 1991 49 56
5. A. Lindgren, J. Emneus, G. Marko-Varga, H. Irth, A. Oosterkamp, and S. Eremin Optimisation of a heterogeneous non-competitive flow immunoassay comparing fluorescein, peroxidase, and alkaline phosphatase as labels J. Immunol. Methods 211 1998 33 42
6. D.E. Wampler, E.D. Lehman, J. Boger, W.J. McAleer, and E.M. Scolnick Multiple chemical forms of hepatitis B surface antigen Proc. Natl. Acad. Sci. USA 82 1985 6830 6834
7. O.H. Lowry, N.J. Rosebrough, A.L. Farr, and R.J. Randall Protein measurement with the folin phenol reagent J. Biol. Chem. 193 1951 265 275
8. J.R. Crowther (Ed.), The ELISA Guide Book: Methods in Molecular Biology, vol. 149, Humana Press, Totowa, NJ, 2001, pp. 233-300.
9. V. Towne, M. Will, B. Oswald, and Q. Zhao Complexities in horseradish peroxidase-catalyzed oxidation of dihydroxyphenoxazine derivatives: appropriate ranges for pH values and hydrogen peroxide concentrations in quantitative analysis Anal. Biochem. 334 2004 290 296
10. M. Zhou, Z. Diwu, N. Panchuk-Voloshina, and R. Haugland A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: applications in detecting the activity of phagocyte NADPH oxidase and other oxidases Anal. Biochem. 253 1997 162 168
11. P. Wentworth Jr., L.H. Jones, A.D. Wentworth, X. Zhu, N.A. Larsen, I.A. Wilson, X. Xu, W.A. Goddard III, K.D. Janda, A. Eschenmosed, and R.A. Lerner Antibody catalysis of the oxidation of water Science 293 2001 1806 1811
12. K. Zaitsu, and Y. Ohkura New fluorogenic substrates for horseradish peroxidase: rapid and sensitive assays for hydrogen peroxide and the peroxidase Anal. Biochem. 109 1980 109 113
13. D.B. Craig, E.A. Arriaga, J.C.Y. Wong, H. Lu, and N.J. Dovichi Studies on single alkaline phosphatase molecules: reaction rate and activation energy of a reaction catalyzed by a single molecule and the effect of thermal denaturation-the death of an enzyme J. Am. Chem. Soc. 118 1996 5245
14. D.B. Craig, J.C.Y. Wong, and N.J. Dovichi Detection of attomolar concentrations of alkaline phosphatase by capillary electrophoresis using laser-induced fluorescence detection Anal. Chem. 68 1996 697 700
15. J.H. Zhang, T.D.Y. Chung, and K.R. Oldenburg A simple statistical parameter for use in evaluation and validation of high throughput screening assays J. Biomol. Screen. 4 1999 67 73
16. R.J. Leatherbarrow, GraFit software version 5.0.6, Erithacus Software Limited, Middlesex, UK, copyright 1989-2003.
17. D. Rodbard, D.M. Hutt, Statistical analysis of radioimmunoassay and immunoradiometric (labeled antibody) assays: a generalized weighted, iterative, least squares method for logistic curve fitting, in: Radioimmunoassay and Related Procedures in Medicine, International Atomic Energy Agency, Vienna, Austria, 1974, pp. 165-190.
18. S. Christoph, and F.J. Meyer-Almes Novel fluorescence based receptor binding assay method for receptors lacking ligand conjugates with preserved affinity: study on estrogen receptor alpha Biopolymers 72 2003 256 263
19. G. Wu, Y. Yuan, and C.N. Hodge Determining appropriate substrate conversion for enzymatic assays in high-throughput screening J. Biomol. Screen. 8 2003 694 700
20. K.A. Drake, J.H. Zhang, R.K. Harrison, and G.M. McGeehan Development of a homogeneous, fluorescence resonance energy transfer-based in vitro recruitment assay for peroxisome proliferator-activated receptor delta via selection of active LXXLL coactivator peptides Anal. Biochem. 304 2002 63 69
21. N. Bouaicha, I. Maatouk, G. Vincent, and Y. Levi A colorimetric and fluorometric microplate assay for the detection of microcystin-LR in drinking water without preconcentration Food Chem. Toxicol. 40 2002 1677 1683
22. M. Banerjee, B.A. Whitton, and D.D. Wynn-Williams Phosphatase activities of endolithic communities in rocks of the Antarctic dry valleys Microb. Ecol. 39 2000 80 91
23. T.T. Yang, P. Sinai, P.A. Kitts, and S.R. Kain Quantification of gene expression with a secreted alkaline phosphatase reporter system BioTechniques 23 1997 1110 1114
24. N.J. Gleason, and J.D. Carbeck Measurement of enzyme kinetics using microscale steady-state kinetic analysis Langmuir 20 2004 6374 6381
25. M.C. Gutierrez, A. Sleegers, H.D. Simpson, V. Alphand, and R. Furstoss The first fluorogenic assay for detecting a Baeyer-Villigerase activity in microbial cells Org. Biomol. Chem. 1 2003 3500 3506
26. A.L. James, J.D. Perry, C. Jay, D. Monget, J.W. Rasburn, and F.K. Gould Fluorogenic substrates for the detection of microbial nitroreductases Lett. Appl. Microbiol. 33 2001 403 408
27. I. Leclercq, J.P. Desager, C. Vandenplas, and Y. Horsmans Fast determination of low-level cytochrome P-450 1A1 activity by high-performance liquid chromatography with fluorescence or visible absorbance detection J. Chromatogr. B Biomed. Appl. 681 1996 227 232
28. Q. Zhao, and R. Sitrin Surface phosphophilicity of aluminum-containing adjuvants probed by their efficiency for catalyzing the P-O bond cleavage with chromogenic and fluorogenic substrates Anal. Biochem. 295 2001 76 81
29. B. Friguet, A.F. Chaffotte, L. Djavadi-Ohaniance, and M.E. Goldberg Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay J. Immunol. Methods 77 1985 305 319