Evaluation of a Commercial Kit for Glutathione Estimation in Cellular Systems

Alternatives to Laboratory Animals

Volumn 25, Issue 6, 1997, Pages 667-673

  Atmaca, Mukadder ^a   Hammond, Alison H ^b   Fry, Jeffrey R ^b  

^a DICLE UNIVERSITY SCHOOL OF MEDICINE   (Turkey)

^b UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

Glutathione; GSH; GSH 400; Saville method; Tietze method

Indexed keywords

EID: 2542629688     PISSN: 02611929     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (12)

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2. Saville, B. (1958). A scheme for the colorimetric determination of microgram amounts of thiols. The Analyst 83, 670-672.
3. Tateishi, N., Higashi, T , Shinya, S., Naruse, A & Sakamoto, Y. (1974). Studies on the regulation of GSH level in rat liver. Journal of Biochemistry 75, 93-103.
4. Higashi, T , Furukawa, M , Hikita, K., Naruse, A., Tateishi, N. & Sakamoto, Y (1985). Re-evaluation of protein-bound glutathione in rat liver. Journal of Biochemistry 98, 1661-1667.
5. Hammond, A H & Fry, J.R. (1996). Effects of culture duration, cytochrome P450 inhibition and glutathione depletion on toxicity of diverse xenobiotics. Toxicology in Vitro 10, 315-321.
6. Malorni, W., Rivabene, R., Matarrese, P (1995). The antioxidant N-acetyl-cysteine protects cultured epithelial cells from menadione-induced cytopathology Chemico-Biological Interactions 96, 113-123
7. Reese, J.A & Byard, J L. (1981). Isolation and culture of adult hepatocytes from liver biopsies. In Vitro 17, 935-940
8. Tietze, F (1969), Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Analytical Biochemistry 27, 502-522.
9. Ookhtens, M., Hobdy, K , Corvasce, M.C , Aw, TY & Kaplowitz, N (1985). Sinusoidal efflux of GSH in the perfused rat liver: evidence for carrier-mediated process Journal of Clinical Investigation 75, 258-265.
10. Jaeschke, H (1990) Glutathione disulphide as index of oxidant stress in rat liver during hypoxia American Journal of Physiology - Gastrointestinal and Liver Physiology 258, G499-G505
11. LIU, J H., Sato, C , Takeno, T & Marumo, F (1993) Characterization of vasopressin-mediated GSH efflux in Hep G2 cells: significance of protein kinase C Life Sciences 52, 1217-1223
12. Atmaca, M & Fry, J R (1996) Adenosine-mediated inhibition of glutathione synthesis in rat isolated hepatocytes Biochemical Pharmacology 52, 1423-1428