Adenosine-mediated inhibition of glutathione synthesis in rat isolated hepatocytes

Biochemical Pharmacology

Volumn 52, Issue 9, 1996, Pages 1423-1428

  Atmaca, Mukadder ^a   Fry, Jeffrey R ^a  

^a QUEEN S MEDICAL CENTRE   (United Kingdom)

Author keywords

adenosine; glutathione synthesis; hepatocytes; inhibition; S adenosylhomocysteine hydrolase

Indexed keywords

2 CHLOROADENOSINE; 3,7 DIMETHYL 1 PROPARGYLXANTHINE; 5' DEOXYADENOSINE; 6 N CYCLOPENTYLADENOSINE; 8 CYCLOPENTYL 1,3 DIPROPYLXANTHINE; ACETYLCYSTEINE; ADENOSINE; ADENOSINE DERIVATIVE; ADENOSINE RECEPTOR BLOCKING AGENT; ADENOSINE RECEPTOR STIMULATING AGENT; ADENOSINE TRIPHOSPHATE; ADENOSYLHOMOCYSTEINASE; ADRENALIN; CORDYCEPIN; CYSTEINE; DEOXYADENOSINE; GLUTAMATE CYSTEINE LIGASE; GLUTATHIONE; MALEIC ACID DIETHYL ESTER; MENADIONE; METHIONINE; SERINE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL METABOLISM; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOTOXICITY; DEPLETION; DRUG MECHANISM; DRUG POTENTIATION; LIVER CELL CULTURE; MALE; NONHUMAN; OXIDATION REDUCTION STATE; PRIORITY JOURNAL; RAT;

2-CHLOROADENOSINE; ADENOSINE; ADENOSINE TRIPHOSPHATE; ANIMALS; GLUCOSE; GLUTATHIONE; LIVER; MALE; METHIONINE; RATS; RATS, WISTAR; RECEPTORS, PURINERGIC P1; VITAMIN K;

EID: 0030575931     PISSN: 00062952     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-2952(96)00504-7     Document Type: Article

References (17)

1. 1. Deneke SM and Fanburg BL, Regulation of cellular glutathione. Am J Physiol 257: L163-L173, 1989.
2. 2. Kaplowitz N, Aw TY and Ookhtens M, The regulation of hepatic glutathione. Ann Rev Pharmacol Toxicol 25: 715-744, 1985.
3. 3. Rao AM, Drake MR and Stipanuk MH, Role of the transsulfuration pathway and of γ-cystathionase activity in the formation of cysteine and sulfate from methionine in rat hepatocytes. J Nutr 120: 837-845, 1990.
4. 4. Bontemps F, Vincent MF and Van Den Berghe G, Mechanisms of elevation of adenosine levels in anoxic hepatocytes. Biochem J 290: 671-677, 1993.
5. 5. Ramkumar V, Nie Z, Rybak LP and Maggirwar SB, Adenosine, antioxidant enzymes and cytoprotection. Trends Pharmacol Sci 16: 283-285, 1995.
6. 6. Reese JA and Byard JL, Isolation and culture of adult hepatocytes from liver biopsies. In Vitro 17: 935-940, 1981.
7. 7. Kreamer BL, Staecker JL, Sawada N, Sattler GL, Hsia MT and Pitot HC, Use of a low-speed iso-density percoll centrifugation method to increase the viability of isolated rat hepatocyte preparations. In Vitro Cell Dev Biol 22: 201-211, 1986.
8. 8. Saville B, A scheme for the colorimetric determination of microgram amounts of thiols. Analyst 83: 670-672, 1958.
9. 9. Tietze F, Enzymatic method for quantitative determination of nanogram amounts of total and oxidized glutathione: application to mammalian blood and other tissues. Anal Biochem 27: 502-522, 1969.
10. 10. Hammond AH, Garle MJ and Fry JR, Mechanism of toxicity of precocene II in rat hepatocyte cultures. J Biochem Toxicol 10: 265-273, 1995.
11. 11. Morimoto Y, Wettstein M and Haussinger D, Hepatocyte heterogeneity in response to extracellular adenosine. Biochem J 293: 573-581, 1993.
12. 12. Tinton S and Buc-Calderon P, Inhibition of protein synthesis induced by adenine nucleotides requires their metabolism into adenosine. Biochem Pharmacol 50: 481-488, 1995.
13. 13. Fabianowska-Majewska K, Duley JA and Simmonds HA, Effects of novel anti-viral adenosine analogues on the activity of S-adenosylhomocysteine hydrolase from human liver. Biochem Pharmacol 48: 897-903, 1994.
14. 14. Tinton S and Buc-Calderon P, Homocysteine enhances the inhibitory effect of extracellular adenosine on the synthesis of proteins in isolated rat hepatocytes, Biochem J 310: 893-896, 1995.
15. 15. Tinton SA, Chow SC, Buc-Calderon PM, Kass GEN and Orrenius S, Adenosine inhibits protein synthesis in isolated rat hepatocytes: evidence for a lack of involvement of intracellular calcium in the mechanism of inhibition. Eur J Biochem 229: 419-425, 1995.
16. 16. O'Brien PJ, Molecular mechanisms of quinone cytotoxicity. Chem Biol Interact 80: 1-41, 1991.
17. 17. Lu SC, Kuhlenkamp J, Garcia-Ruiz C and Kaplowitz N, Hormone-mediated down-regulation of hepatic glutathione synthesis in the rat. J Clin Invest 88: 260-269, 1991.