A proteomic approach to identify phosphoproteins encoded by cDNA libraries

Analytical Biochemistry

Volumn 329, Issue 2, 2004, Pages 289-292

  Shi, Xudong ^a   Belton Jr , Robert J ^a   Burkin, Heather R ^a   Vieira, Ana P ^a   Miller, David J ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; CLONING; MASS SPECTROMETRY; PHAGE DISPLAY; PHOSPHORYLATION; POLYMERASE CHAIN REACTION; PROTEOMICS;

ENZYMATIC DIGESTIONS; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; PEPTIDE SEQUENCING; PHOSPHORYLATED PROTEINS; PHOSPHORYLATION SITES; PROTEOMIC APPROACHES; RAPID IDENTIFICATION; TANDEM MASS SPECTROMETRIC TECHNIQUES;

ANTIBODIES;

ANTIBODY; AUTOANTIGEN; CELL MEMBRANE PROTEIN; COMPLEMENTARY DNA; GLYCOGEN PHOSPHORYLASE; HYBRID PROTEIN; N METHYL DEXTRO ASPARTIC ACID RECEPTOR; NUCLEOLIN; PAXILLIN; PHENYLALANINE 4 MONOOXYGENASE; PHOSPHOPROTEIN; PHOSPHORYLASE KINASE; PHOSPHOTYROSINE; PLECKSTRIN; PROTAMINE; PROTEIN KINASE LCK; PROTEIN SHC;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DNA LIBRARY; DNA SCREENING; ENZYMATIC ASSAY; GENE AMPLIFICATION; GENE SEQUENCE; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; IMMUNOBLOTTING; MOLECULAR CLONING; NONHUMAN; PHAGE DISPLAY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; PROTEOMICS; SEQUENCE ANALYSIS; TANDEM MASS SPECTROMETRY;

EID: 2542462392     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2004.03.033     Document Type: Article

References (15)

1. Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Phosphoproteome analysis by mass spectrometry and its application to Saccaromyces cerevisiae. Nat. Biotechnol. 20:2002;301-305
2. Conrads T.P., Issaq H.J., Veenstra T.D. New tools for quantitative phosphoproteome analysis. Biochem. Biophys. Res. Commun. 290:2002;885-890
3. Stern D.F. Phosphoproteomics. Exp. Mol. Pathol. 70:2001;327-331
4. Stensballe A., Andersen S., Jensen O.N. Characterization of phosphoproteins from electrophoretic gels by nanoscale Fe(III) affinity chromatography with off-line mass spectrometry analysis. Proteomics. 1:2001;207-222
5. Porath J., Carlsson J., Olsson I., Belfrage G. Metal chelate affinity chromatography, a new approach to protein fractionation. Nature. 258:1975;598-599
6. Powell K.A., Valova V.A., Malladi C.S., Jensen O.N., Larsen M.R., Robinson P.J. Phosphorylation of dynamin I on Ser-795 by protein kinase C blocks its association with phospholipids. J. Biol. Chem. 275:2000;11610-11617
7. Neville D.C.A., Rozanas C.R., Price E.M., Gruis D.B., Verkman A.S., Townsend R.R. Evidence for phosphorylation of serine 753 in CFTR using a novel metal-ion affinity resin and matrix-assisted laser desorption mass spectrometry. Protein Sci. 6:1997;2436-2445
8. Nuhse T.S., Stensballe A., Jensen O.N., Peck S.C. Large-scale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry. Mol. Cell. Proteomics. 2003
9. Ficarro S., Chertihin O., Westbrook V.A., White F., Jayes F., Kalab P., Marto J.A., Shabanowitz J., Herr J.C., Hunt D.F., Visconti P.E. Phosphoproteome analysis of capacitated human sperm. Evidence of tyrosine phorphorylation of A Kinase-Anchoring Protein 3 and valosin-containing protein/p97 during capacitation. J. Biol. Chem. 278:2003;11579-11589
10. Muszynska G., Dobrowolska G., Medin A., Ekman P., Porath J.O. Model studies on iron(III) ion affinity chromatography. II. Interaction of immobilized iron(III) ions with phosphorylated amino acids, peptides and proteins. J. Chromatogr. 604:1992;19-28
11. Posewitz M.C., Tempst P. Immobilized gallium(III) affinity chromatography of phosphopeptides. Anal. Chem. 71:1999;2883-2892
12. Cozzone A.J. Regulation of acetate metabolism by protein phosphorylation in enteric bacteria. Annu. Rev. Microbiol. 52:1998;127-164
13. Robertson E.S., Nicholson A.W. Protein kinase of bacteriophage T7 induces the phosphorylation of only a small number of proteins in the infected cell. Virology. 175:1990;525-534
14. Mijakovic I., Poncet S., Boel G., Maze A., Gillet S., Jamet E., Decottignies P., Grangeasse C., Doublet P., Le Marechal P., Deutscher J. Transmembrane modulator-dependent bacterial tyrosine kinase activates UDP-glucose dehydrogenases. EMBO J. 22:2003;4709-4718
15. Kennelly P.J. Archael protein kinases and protein phosphatases: insights from genomics and biochemistry. Biochem. J. 370:2003;373-389