Down-regulation of the GTPase RhoB might be involved in the pre-apoptotic phenotype of osteoarthritic chondrocytes

Frontiers in Bioscience

Volumn 9, Issue , 2004, Pages 827-833

  Gebhard, Pia M ^a   Söder, Stephan ^a   Bau, Brigitte ^a   Aigner, Thomas ^a  

^a UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

Author keywords

Apoptosis; Cartilage; Cell Senescence; Chondrocyte; Osteoarthritis

Indexed keywords

RHO GUANOSINE TRIPHOSPHATASE; INTERLEUKIN 1; RHOB GUANINE NUCLEOTIDE BINDING PROTEIN;

APOPTOSIS; ARTICLE; ARTICULAR CARTILAGE; CARTILAGE CELL; CELL ACTIVATION; CELL CYCLE; CELL ISOLATION; CELL STIMULATION; CONFOCAL LASER MICROSCOPY; DNA DAMAGE; DNA HYBRIDIZATION; DNA MICROARRAY; DNA SYNTHESIS; DOWN REGULATION; ELUTION; HISTOCHEMISTRY; HUMAN; IMMUNOHISTOCHEMISTRY; MONOLAYER CULTURE; MORPHOMETRICS; NONHUMAN; OSTEOARTHRITIS; PHENOTYPE; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN INDUCTION; PROTEIN LOCALIZATION; RNA ANALYSIS; RNA ISOLATION; STATISTICAL ANALYSIS; TISSUE SLICE; ADULT; AGED; CONFOCAL MICROSCOPY; DRUG EFFECT; GENE EXPRESSION; GENETICS; METABOLISM; MIDDLE AGED; PATHOLOGY; TISSUE DISTRIBUTION;

EUKARYOTA;

ADULT; AGED; AGED, 80 AND OVER; APOPTOSIS; CARTILAGE, ARTICULAR; CHONDROCYTES; DOWN-REGULATION; GENE EXPRESSION; HUMANS; INTERLEUKIN-1; MICROSCOPY, CONFOCAL; MIDDLE AGED; OSTEOARTHRITIS; PHENOTYPE; RHOB GTP-BINDING PROTEIN; TISSUE DISTRIBUTION;

EID: 2542425577     PISSN: 27686701     EISSN: 27686698     Source Type: Journal    
DOI: 10.2741/1254     Document Type: Article

References (41)

1. Mitrovic, D. Quintero, M. Stankovic, A. Ryckewaert, A. Cell density of adult human femoral condylar articular cartilage. Lab Invest 49:309-316 (1983)
2. Stockwell, R. A. The interrelationship of cell density and cartilage thickness in mammalian articular cartilage. J Anat 109:411-421 (1971)
3. Vignon, E. Arlot, M. Patricot, L. M. Vignon, G. The cell densitiy of human femoral head cartilage. Clin Orthop 121:303-308 (1976)
4. Blanco, F. J. Guttian, R. Vázquez-Martul, E. de Toro, F. J. Galdo, F. Osteoarthritic chondrocytes die by apoptosis. Arthritis Rheum 41:284-289 (1998)
5. Hashimoto, S. Ochs, R. L. Komiya, S. Lotz, M. Linkage of chondrocyte apoptosis and cartilage degradation in human osteoarthritis. Arthritis Rheum 41:1632-1638 (1998)
6. Aigner, T. Hemmel, M. Neureiter, D. Gebhard, P. M. Zeiler, G. Kirchner, T. McKenna, L. A. Apoptotic cell death is not a widespread phenomenon in normal aging and osteoarthritic human articular knee cartilage: A study of proliferation, programmed cell death (apoptosis), and viability of chondrocytes in normal and osteoarthritic human knee cartilage. Arthritis Rheum 44:1304-1312 (2001)
7. Aigner, T. Kim, A. H. Apoptosis and cellular vitality - Issues in osteoarthritic cartilage degeneration. Arthritis Rheum 46(8):1986-1996 (2002)
8. Aigner, T. Zien, A. Gehrsitz, A. Gebhard, P. M. McKenna, L. A. Anabolic and catabolic gene expression pattern analysis in normal versus osteoarthritic cartilage using complementary DNA-array technology. Arthritis Rheum 44:2777-2789 (2001)
9. Nakamura, T. Asano, M. Shindo-Okada, N. Nishimura, S. Monden, Y. Cloning of the RhoB gene from the mouse genome and characterization of its promoter region. Biochem Biophys Res Commun 226:688-694 (1996)
10. Hall, A. Rho GTPases and the actin cytoskeleton. Science 279:509-514 (1998)
11. Fukata. M. Kaibuchi, K. Rho-family GTPases in cadherin-mediated cell-cell adhesion. Nat Rev Mol Cell Biol 2:887-897 (2001)
12. Etienne, J. C. Hall, A. Rho GTPases in cell biology. Nature 420:629-635 (2002)
13. Engel, M. E. Datta, P. K. Moses, H. L. RhoB is stabilized by transforming growth factor beta and antagonizes transcriptional activation. J Biol Chem 273:9921-9926 (1998)
14. Adnane, J. Seijo, E. Chen, Z. Bizouarn, F. Leal, M. Sebti, S. M. Munoz-Antonia, T. RhoB, not RhoA, represses the transcription of the transforming growth factor beta type II receptor by a mechanism involving activator protein 1. J Biol Chem 277:8500-8507 (2002)
15. Wang, L. Almqvist, K. F. Veys, E. M. Verbruggen, G. Control of extracellular matrix homeostasis of normal cartilage by a TGFbeta autocrine pathway. Validation of flow cytometry as a tool to study chondrocyte metabolism in vitro. Osteoarthritis Cartilage 10:188-198 (2002)
16. Prendergast, G. C. Actin' up: RhoB in cancer and apoptosis. Nat Rev Cancer 1:162-168 (2001)
17. Jahner, D. Hunter, T. The ras-related gene rhoB is an immediate-early gene inducible by v-Fps, epidermal growth factor, and platelet-derived growth factor in rat fibroblasts. Mol Cell Biol 11:3682-3690 (1991)
18. Zalcman, G. Closson, V. Linares-Cruz, G. Lerebours, F. Honore, N. Tavitian, A. Olofsson, B. Regulation of Ras-related RhoB protein expression during the cell cycle. Oncogene 10:1935-1945 (1995)
19. McKenna, L. A. Gehrsitz, A. Soeder, S. Eger, W. Kirchner, T. Aigner, T. Effective isolation of high quality total RNA from human adult articular cartilage. Analytical Biochemistry 286:80-85 (2000)
20. Gehrsitz, A. McKenna, L. A. Soeder, S. Kirchner, T. Aigner, T. Isolation of RNA from small human articular cartilage specimens allows quantification of mRNA expression levels in local articular cartilage defects. J Orthop Res 19:478-482 (2001)
21. Aigner, T. Zhu, Y. Chanksi, H. Matsen, F. A. Maloney, W. J. Sandell, L. J. Reexpression of procollagen type IIA by adult articular chondrocytes in osteoarthritis cartilage. Arthritis Rheum 42:1443-1450 (1999)
22. Adamson, P. Paterson, H. F. Hall, A. Intracellular localization of the P21 rho proteins. J Cell Biol 119:617-627 (1992)
23. Robertson, D. Paterson, H. F. Adamson, P. Hall, A. Monaghan, P. Ultrastructural localization of ras-related proteins using epitope-tagged plasmids. J Histochem Cytochem 43:471-480 (1995)
24. Bau, B. Gebhard, P.M. Haag, J. Knorr, T. Bartnik, E. Aigner, T. Relative messenger RNA expression profiling of collagenases and aggrecanases in human articular chondrocytes in vivo and in vitro. Arthritis Rheum 46:2648-2657 (2002)
25. Gebhard, P. M. Gehrsitz, A. Bau, B. Söder, S. Eger, W. Aigner, T. Quantification of expression levels of cellular differentiation markers does not support a general shift in the cellular phenotype of osteoarthritic chondrocytes. J Orthop Res 21:96-101 (2003)
26. Trapp, T. Olah, L. Holker, I. Besselmann, M. Tiesler, C. Maeda, K. Hossmann, K. A. GTPase RhoB: an early predictor of neuronal death after transient focal ischemia in mice. Mol Cell Neurosci 17:883-894 (2001)
27. Fritz, G. Kaina, B. Ras-related GTPase Rhob represses NF-kappaB signaling. J Biol Chem 276:3115-3122 (2001)
28. Teramoto, H. Crespo, P. Coso, O. A. Igishi, T. Xu, N. Gutkind, J. S. The small GTP-binding protein rho activates c-Jun N-terminal kinases/stress- activated protein kinases in human kidney 293T cells. Evidence for a Pak-independent signaling pathway. J Biol Chem 271:25731-25734 (1996)
29. Denhardt, D. T. Signal-transducing protein phosphorylation cascades mediated by Ras/Rho proteins in the mammalian cell: the potential for multiplex signalling. Biochem J 318 (Pt 3):729-747 (1996)
30. Kracht, M. Saklatvala, J. Transcriptional and post-transcriptional control of gene expression in inflammation. Cytokine 20:91-106 (2002)
31. Liu, A. X. Cerniglia, G. J. Bernhard, E. J. Prendergast, G. C. RhoB is required to mediate apoptosis in neoplastically transformed cells after DNA damage. Proc Natl Acad Sci U S A 98:6192-6197 (2001)
32. Kim, H. A. Lee, Y. J. Seong, S-C. Choe, K. W. Song, Y. W. Apoptotic chondrocyte death in human osteoarthritis. J Rheumatol 27:455-462 (2000)
33. Fritz, G. Kaina, B. rhoB encoding a UV-inducible Ras-related small GTP-binding protein is regulated by GTPases of the Rho family and independent of JNK, ERK, and p38 MAP kinase. J Biol Chem 272:30637-30644 (1997)
34. Fritz, G. Kaina, B. Aktories, K. The ras-related small GTP-binding protein RhoB is immediate-early inducible by DNA damaging treatments. J Biol Chem 270:25172-25177 (1995)
35. Aigner, T. Dudhia, J. Phenotypic modulation of chondrocytes as a potential therapeutic target in osteoarthritis: a hypothesis. Ann Rheum Dis 56:287-291 (1997)
36. Rothwell, A. G. Bentley, G. Chondrocyte multiplication in osteoarthritic articular cartilage. J Bone Joint Surg 55-B:588-594 (1973)
37. Mankin, H. J. Dorfman, H. Lippiello, L. Zarins, A. Biochemical and metabolic abnormalities in articular cartilage from osteoarthritic human hips. J Bone Joint Surg 53-A:523-537 (1971)
38. Fritz, G. Kaina, B. Ras-related GTPase RhoB forces alkylation-induced apoptotic cell death. Biochem Biophys Res Commun 268:784-789 (2000)
39. Kusuzaki, K. Sugimoto, S. Takeshita, H. Murata, H. Hashiguchi, S. Nozaki, T. Emoto, K. Ashihara, T. Hirasawa, Y. DNA cytofluorometric analysis of chondrocytes in human articular cartilages under normal aging or arthritic conditions. Osteoarthritis Cartilage 9:664-670 (2001)
40. Sandell, L. J. Aigner, T. Articular cartilage and changes in arthritis. An introduction: cell biology of osteoarthritis. Arthritis Res 3:101-113 (2001)
41. Martin, J. A. Buckwalter, J. A. Aging, articular cartilage chondrocyte senescence and osteoarthritis. Biogerontology 3:257-264 (2002)