Isolation of RNA from small human articular cartilage specimens allows quantification of mRNA expression levels in local articular cartilage defects

Journal of Orthopaedic Research

Volumn 19, Issue 3, 2001, Pages 478-481

  Gehrsitz, Angelika ^a   McKenna, Louise A ^a   Söder, Stefan ^a   Kirchner, Thomas ^a   Aigner, Thomas ^a  

^a UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CARTILAGE; COLLAGEN; GENES;

CARTILAGE DEGENERATION;

RNA;

AGGRECAN; COLLAGEN TYPE 2; MESSENGER RNA; RNA;

ADULT; ARTICLE; ARTICULAR CARTILAGE; CARTILAGE DEGENERATION; CLINICAL PROTOCOL; CONTROLLED STUDY; GENE EXPRESSION; HUMAN; HUMAN TISSUE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA ANALYSIS; TECHNIQUE; TISSUE; WEIGHT;

EID: 0034998679     PISSN: 07360266     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0736-0266(00)90028-7     Document Type: Article

References (9)

1. Adams M.E., Huang D.Q., Yao L.Y., Sandell L.J. Extraction and isolation of mRNA from adult articular cartilage. Anal Biochem. 202:1992;89-95.
2. Aigner T., Stöß H., Weseloh G., Zeiler G., von der Mark K. Activation of collagen type II expression in osteoarthritic and rheumatoid cartilage. Virchows Arch B. 62:1992;337-345.
3. Aigner T., Vornehm S.I., Zeiler G., Dudhia J., von der Mark K., Bayliss M.T. Suppression of cartilage matrix gene expression in upper zone chondrocytes of osteoarthritic cartilage. Arthritis Rheum. 40:1997;562-569.
4. Carney S.L., Muir H. The structure and function of cartilage proteoglycans. Physiol Rev. 68:1988;858-910.
5. Cs-Szabó G., Melching L.I., Roughley P.J., Glant T.T. Changes in messenger RNA and protein levels of proteoglycans and link protein in human osteoarthritic cartilage samples. Arthritis Rheum. 40:1997;1037-1045.
6. Mankin H.J., Dorfman H., Lippiello L., Zarins A. Biochemical and metabolic abnormalities in articular cartilage from osteo-arthritic human hips. J Bone and J Surg. 53-A:1971;523-537.
7. Matyas J.R., Adams M.E., Huang D., Sandell L.J. Discoordinate gene expression of aggrecan and type II collagen in experimental osteoarthritis. Arthritis Rheum. 38:1995;420-425.
8. Melching L.I., Cs-Szabó G., Roughley P.J. Analysis of proteoglycan messages in human articular cartilage by a compitative PCR technique. Matrix Biol. 16:1997;1-11.
9. Smale G., Sasse J. RNA isolation from cartilage using density gradient centrifugation in cesium trifluoroacetate: An RNA preparation tequnique effective of high proteoglycan content. Anal Biochem. 203:1992;352-356.