Rapid quantification of bacteria in activated sludge using fluorescence in situ hybridization and epifluorescence microscopy

Water Research

Volumn 39, Issue 16, 2005, Pages 3837-3848

  Hug, Thomas ^a   Gujer, Willi ^a   Siegrist, Hansruedi ^a  

^a SWISS FEDERAL INSTITUTE OF AQUATIC SCIENCE AND TECHNOLOGY   (Switzerland)

Author keywords

Fluorescence in situ hybridization (FISH); Foaming; Microthrix parvicella; Nocardioform actinomycetes; Quantification; Scum

Indexed keywords

FLUORESCENCE; FOAMS; IMAGE PROCESSING; MICROSCOPIC EXAMINATION; SEWAGE SLUDGE; WATER ANALYSIS;

FLUORESCENCE IN SITU HYBRIDIZATION (FISH); FOAMING; MICROTHRIX PARVICELLA; NONCARDIOFORM ACTINOMYCETES; QUANTIFICATION; SCUM;

BACTERIA;

ACTIVATED SLUDGE; BACTERIUM;

ACTINOMYCES; ACTINOMYCETALES; ACTIVATED SLUDGE; ARTICLE; BACTERIAL STRAIN; BACTERIUM DETECTION; CELL DIFFERENTIATION; EPIFLUORESCENCE MICROSCOPY; FLUORESCENCE; FLUORESCENCE IN SITU HYBRIDIZATION; HYBRIDIZATION; IMAGE ANALYSIS; MICROTHRIX; MORPHOTYPE; NONHUMAN; PHOTOGRAPHY; PRIORITY JOURNAL;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); CANDIDATUS MICROTHRIX PARVICELLA;

EID: 24944579922     PISSN: 00431354     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.watres.2005.07.013     Document Type: Article

References (20)

1. R.I. Amann, B.J. Binder, R.J. Olson, S.W. Chisholm, R. Devereux, and D.A. Stahl Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations Appl. Environ. Microbiol. 56 6 1990 1919 1925
2. C. Beimfohr, A. Krause, R. Amann, W. Ludwig, and K.-H. Schleifer In situ identification of Lactococci, Enterococci and Streptococci Syst. Appl. Microbiol. 16 1993 450 456
3. T. Bouchez, D. Patureau, P. Dabert, S. Juretschko, J. Doré, P. Delgenes, R. Moletta, and M. Wagner Ecological study of a bioaugmentation failure Environ. Microbiol. 2 2 2000 179 190
4. H. Daims Cultivation-independent, semiautomatic determination of absolute bacterial cell numbers in environmental samples by fluorescent in situ hybridization Appl. Environ. Microbiol. 67 12 2001 5810 5818
5. H. Daims, A. Bruehl, R. Amann, K.-H. Schleifer, and M. Wagner The domain-specific probe EUB338 is insufficient for the detection of all bacteria: development and evaluation of a more comprehensive probe set Syst. Appl. Microbiol. 22 3 1999 434 444
6. R.J. Davenport, T.P. Curtis, M. Goodfellow, F.M. Stainsby, and M. Bingley Quantitative use of fluorescent in situ hybridization to examine relationships between mycolic acid-containing actinomycetes and foaming in activated sludge plants Appl. Environ. Microbiol. 66 3 2000 1158 1166
7. F.L. de los Reyes III, and L. Raskin Role of filamentous microorganisms in activated sludge foaming: Relationship of mycolata levels to foaming initiation and stability Water Res. 36 2 2002 445 459
8. F.L. de los Reyes, W. Ritter, and L. Raskin Group-specific small-subunit rRNA hybridization probes to characterize filamentous foaming in activated sludge systems Appl. Environ. Microbiol. 63 3 1997 1107 1117
9. B. Efron, and R.J. Tibshirani An Introduction to the Bootstrap 1993 Chapman & Hall New York
10. D.H. Eikelboom Process control of activated sludge plants by microscopic investigation first ed 2000 IWA Publishing London
11. D.H. Eikelboom, and H.J.J. van Buijsen Microscopic Sludge Investigation Manual second ed 1981 Research Institute for Environmental Hygiene TNO. Water and Soil Division, TU Delft Delft, NL
12. R. Erhart, D. Bradford, R.J. Seviour, R. Amann, and L.L. Blackall Development and use of fluorescent in situ hybridization probes for the detection and identification of "Microthrix parvicella" in activated sludge Syst. Appl. Microbiol. 20 1997 310 318
13. T. Hug, M. Ziranke, and H. Siegrist Dynamics of population and scumming on a full-scale wastewater treatment plant in Switzerland Acta Hydrochim. Hydrobiol. 33 3 2005 216 222
14. D. Jenkins, M.G. Richard, and G.T. Daigger Manual on the Causes and Control of Activated Sludge Bulking and Foaming second ed 1993 Lewis Publisher, Inc. Chelsea, Michigan USA, Chelsea
15. M. Kuehn, M. Hausner, H.-J. Bungartz, M. Wagner, P.A. Wilderer, and S. Wuertz Automated confocal laser scanning microscopy and semiautomated image processing for analysis of biofilms Appl. Environ. Microbiol. 64 11 1998 4115 4127
16. R. Manser, K. Muche, W. Gujer, and H. Siegrist A rapid method to quantify nitrifiers in activated sludge Water Res. 39 2005 1585 1593
17. W. Manz, R. Amann, W. Ludwig, M. Wagner, and K.-H. Schleifer Phylogenetic oligodeoxynucleotide probes for the major subclasses of Proteobacteria: problems and solutions Syst. Appl. Microbiol. 15 1992 593 600
18. M. Wagner, A. Loy, R. Nogueira, U. Purkhold, N. Lee, and H. Daims Microbial community composition and function in wastewaer treatment plants Antonie van Leeuwenhoek 81 2002 665 680
19. G. Wallner, R. Amann, and W. Beisker Optimizing fluorescent in situ hybridization with rRNA-targeted oligonucleotide probes for flow cytometric identification of microorganisms Cytometry 14 2 1993 136 143
20. P.A. Wilderer, H.-J. Bungartz, H. Lemmer, M. Wagner, J. Keller, and S. Wuertz Modern scientific methods and their potential in wastewater science and technology Water Res. 36 2002 370 393