Purification, kinetic and thermodynamic studies of a new ribonuclease from a mutant of Aspergillus niger

Journal of Biotechnology

Volumn 119, Issue 4, 2005, Pages 348-356

  Xiong, Ya Hong ^a,b   Liu, Jian Zhong ^a   Song, Hai Yan ^a   Ji, Liang Nian ^a  

^a SUN YAT SEN UNIVERSITY   (China)

^b SOUTH CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

Aspergillus niger; Kinetics; Purification; Ribonuclease; Thermodynamic parameters

Indexed keywords

BACTERIA; BIOTECHNOLOGY; ENZYMES; ION EXCHANGE; MOLECULAR WEIGHT; PURIFICATION; REACTION KINETICS; COMPUTATION THEORY; COMPUTATIONAL GEOMETRY; DEFORMATION; GALERKIN METHODS; LEAST SQUARES APPROXIMATIONS; PROBLEM SOLVING;

ASPERGILLUS NIGER; KINETICS; RIBONUCLEASE; THERMODYNAMIC PARAMETERS; GEOMETRICALLY NONLINEAR PROBLEMS; MOVING LEAST SQUARE APPROXIMATION; TOTAL LAGRANGIAN METHOD;

THERMODYNAMICS; NONLINEAR SYSTEMS;

2 DIETHYLAMINOETHANOL; AMMONIUM SULFATE; CELLULOSE; FUNGAL ENZYME; MUTANT PROTEIN; RIBONUCLEASE; RNA;

ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; ASPERGILLUS NIGER; BINDING AFFINITY; CATALYSIS; DENATURATION; ENTHALPY; ENTROPY; ENZYME ACTIVATION; ENZYME DENATURATION; ENZYME KINETICS; ENZYME MECHANISM; ENZYME PURIFICATION; HYDROLYSIS; ISOELECTRIC POINT; KINETICS; MICHAELIS CONSTANT; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PRECIPITATION; PRIORITY JOURNAL; TEMPERATURE DEPENDENCE; THERMODYNAMICS; ULTRAFILTRATION;

ASPERGILLUS NIGER; CATALYSIS; ENZYME ACTIVATION; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; KINETICS; MOLECULAR WEIGHT; MUTATION; RIBONUCLEASES; THERMODYNAMICS;

ASPERGILLUS NIGER;

EID: 24944484235     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2005.04.008     Document Type: Article

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