Target gene localization using laser-capture microdissection and real-time reverse transcription-polymerase chain reaction analysis

Journal of Histotechnology

Volumn 28, Issue 3, 2005, Pages 177-180

  Dalmas, Deidre A ^a   Scicchitano, Marshall S ^a   Zimmerman, Dawn ^a   Tierney, Lauren A ^a   Boyce, Rogely W ^a  

^a GLAXOSMITHKLINE   (United States)

Author keywords

In situ hybridization; Laser capture microdissection; Real time RT PCR

Indexed keywords

MESSENGER RNA; NEUROMEDIN U RECEPTOR; RHO KINASE; UNCLASSIFIED DRUG;

ATHEROSCLEROTIC PLAQUE; CONFERENCE PAPER; CONTROLLED STUDY; ENZYME LOCALIZATION; GENE TARGETING; HUMAN; HUMAN CELL; HUMAN TISSUE; IN SITU HYBRIDIZATION; LASER CAPTURE MICRODISSECTION; NUCLEOTIDE SEQUENCE; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; REACTION ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA DEGRADATION; SENSITIVITY ANALYSIS; TISSUE SECTION; TISSUE SPECIFICITY;

EID: 24644476936     PISSN: 01478885     EISSN: None     Source Type: Journal    
DOI: 10.1179/his.2005.28.3.177     Document Type: Conference Paper

References (10)

1. Yang H, Wanner IB, Roper SD, Chaudaur N: An optimized method for in situ hybridization with signal amplification that allows the detection of rare mRNAs. J Histochem Cytochem 47:431-446, 1999
2. Braissant O, Wahli W: A simplified in situ hybridization protocol using non-radioactively labeled probes to detect abundant and rare mRNAs on tissue sections. Biochemica 1:10-16, 1998
3. Wilkinson DG: In situ hybridization: A practical approach (2nd ed). Oxford University Press, New York, 1995
4. Wang H, Eto M, Steers WD, Somlyo AP, Somlyo A: RhoA-mediated CA2+ sensitization in erectile dysfunction. J Biol Chem 277:30614-30621, 2002
5. Rees RW, Ralph DJ, Royle M, Moncada S, Cellek S: Y-27632, An inhibitor of Rho-kinase, antagonizes noradrenergic contractions in the rabbit and human penile corpus cavernosum. Br J Pharmacol 133:455-458, 2001
6. Killer T, Snell L, Watson P: Microdissection RT-PCR analysis of gene expression in pathologically defined frozen tissues. Biotechniques 21:38-44, 1996
7. Bonner RF, Emmert-Buck M, Cole K, Pohida T, Chuaqui R, Goldstein S, Liotta LA: Laser capture microdissection: molecular analysis of tissue. Science 278:1481-1483, 1997
8. Heid CA, Stevens J, Livak KJ, Williams PM: Real time PCR. Genome Res 6:986-994, 1996
9. Medhurst AD, Harrison DC, Read SJ, Campbell CA, Robbins MJ, Pangalos MN: The use of TaqMan® RT-PCR assays for semiquantitative analysis of gene expression in CNS tissues and disease models. J Neurosci Methods 98:9-20, 2000
10. Zimmerman D, Thomas R, Scicchitano M, Dalmas D, Tierney L, De Boer L, Boyce R, Schwartz L: Automation of nonisotopic in situ hybridization. J Histotechnol 26:181-184, 2003