An optimized method for in situ hybridization with signal amplification that allows the detection of rare mRNAs

Journal of Histochemistry and Cytochemistry

Volumn 47, Issue 4, 1999, Pages 431-445

  Yang, Hui ^a,b   Wanner, Ina B ^a   Roper, Stephen D ^a   Chaudhari, Nirupa ^a  

^a UNIVERSITY OF MIAMI MILLER SCHOOL OF MEDICINE   (United States)

^b CAPITAL MEDICAL UNIVERSITY   (China)

Author keywords

In situ hybridization; Rare mRNA; Sensory transduction; Taste; Tyramide amplification

Indexed keywords

ALKALINE PHOSPHATASE; COMPLEMENTARY DNA; DIGOXIGENIN; GUANINE NUCLEOTIDE BINDING PROTEIN; ISOPROPAMIDE IODIDE; MESSENGER RNA; RIBONUCLEASE;

ANIMAL TISSUE; ARTICLE; FLUORESCENCE IN SITU HYBRIDIZATION; IN SITU HYBRIDIZATION; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; RAT; SENSORY NERVE CELL; SIGNAL NOISE RATIO; SIGNAL TRANSDUCTION; TASTE; TISSUE FIXATION;

ANIMALIA;

EID: 0032962092     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215549904700402     Document Type: Article

References (46)

1. Angerer LM, Angerer RC (1992) In situ hybridization to cellular RNA with radiolabeled RNA probes. In Wilkinson DG, ed. In Situ Hybridization. A Practical Approach. Oxford, IRL Press, 15-32
2. Bigiani A, Delay RJ, Chaudhari N, Kinnamon SC, Roper SD, Roper S (1997) Responses to glutamate in rat taste cells. J Neurophysiol 77:3048-3059
3. Bobrow MN, Litt GJ, Shaughnessy KJ, Mayer PC, Conlon J (1992) The use of catalyzed reporter deposition as a means of signal amplification in a variety of formats. J Immunol Methods 150:145-149
4. Bobrow MN, Shaughnessy KJ, Litt GJ (1991) Catalyzed reporter deposition, a novel method of signal amplification. II. Application to membrane immunoassays. J Immunol Methods 137:103-112
5. Boughter JD Jr, Pumplin DW, Yu CS, Christy RC, Smith DV (1997) Differential expression of α-gustducin in taste bud populations of the rat and hamster. J Neurosci 17:2852-2858
6. Chao J, DeBiasio R, Zhu Z, Giuliano KA, Schmidt BF (1996) Immunofluorescence signal amplification by the enzyme-catalyzed deposition of a fluorescent reporter substrate (CARD). Cytometry 23:48-53
7. Chaudhari N, Beam KG (1993) mRNA for cardiac calcium channel is expressed during development of skeletal muscle. Dev Biol 155:507-515
8. Chaudhari N, Yang H, Lamp C, Delay E, Cartford C, Than T, Roper S (1996) The taste of monosodium glutamate: membrane receptors in taste buds. J Neurosci 16:3817-3826
9. de Haas RR, Verwoerd NP, van der Corput MP, van Gijlswijk RP, Siitari H, Tanke HJ (1996) The use of peroxidase-mediated deposition of biotin-tyramide in combination with time-resolved fluorescence imaging of europium chelate label in immunohistochemistry and in situ hybridization. J Histochem Cytochem 44: 1091-1099
10. Gallyas F, Hsu M, Buzsaki G (1993) Four modified silver methods for thick sections of formaldehyde-fixed mammalian central nervous tissue: 'dark' neurons, perikarya of all neurons, microglial cells and capillaries. J Neurosci Methods 50:159-164
11. Guiot Y, Rahier J (1995) The effects of varying key steps in the non-radioactive in situ hybridization protocol: a quantitative study. Histochem J 27:60-68
12. Hunyady B, Krempels K, Harta G, Mezey E (1996) Immunohistochemical signal amplification by catalyzed reporter deposition and its application in double immunostaining. J Histochem Cytochem 44:1353-1362
13. Kerstens HM, Poddighe PJ, Hanselaar AG (1995) A novel in situ hybridization signal amplification method based on the deposition of biotinylated tyramine. J Histochem Cytochem 43:347-352
14. Landry M, Hökfelt T (1998) Subcellular localization of preprogalanin messenger RNA in perikarya and axons of hypothalamoposthypophyseal magnocellular neurons: an in situ hybridization study. Neuroscience 84:897-912
15. Larsson LI, Hougaard DM (1990) Optimization of non-radioactive in situ hybridization: image analysis of varying pretreatment, hybridization and probe labeling conditions. Histochemistry 93: 347-354
16. Li X-J, Blackshaw S, Snyder SH (1994) Expression and localization of amiloride-sensitive sodium channel indicate a role for non-taste cells in taste perception. Proc Natl Acad Sci USA 91:1814-1818
17. Liposits Z, Petersen SL, Paull WK (1991) Amplification of the in situ hybridization signal by silver post-intensification: the biotin-dUTP-streptavidin-peroxidase diaminobenzidine-silver-gold detection system. Histochemistry 96:339-342
18. Litman P, Barg J, Ginzburg I (1994) Microtubules are involved in the localization of tau mRNA in primary neuronal cell cultures. Neuron 13:1463-1474
19. Litman P, Barg J, Rindzoonski L, Ginzburg I (1993) Subcellular localization of tau mRNA in differentiating neuronal cell culture: implications for neuronal polarity. Neuron 10:627-638
20. Macechko PT, Krueger L, Hirsch B, Erlandsen SL (1997) Comparison of immunologic amplification vs enzymatic deposition of fluorochrome-conjugated tyramide as detection systems for FISH. J Histochem Cytochem 45:359-363
21. McLaughlin SK, McKinnon PJ, Margolskee RF (1992) Gustducin is a taste-cell-specific G protein closely related to the transducins. Nature 357:563-569
22. McLaughlin SK, McKinnon PJ, Robichon A, Spickofsky N, Margolskee RF (1993) Gustducin and transducin: a tale of two G proteins. In Chadwick D, Marsh J, Goude J, eds. The Molecular Basis of Smell and Taste Transduction. Chichester, Wiley 186-200
23. McLaughlin SK, McKinnon PJ, Spickofsky N, Danho W, Margolskee RF (1994) Molecular cloning of G proteins and phosphodiesterases from rat taste cells. Physiol Behav 56:1157-1164
24. Ming D, Ruiz-Avila L, Margolskee RF (1998) Characterization and solubilization of bitter-responsive receptors that couple to gustducin. Proc Natl Acad Sci USA 95:8933-8938
25. Misaka T, Kusakabe Y, Emori Y, Gonoi T, Arai S, Abe K (1997) Taste buds have a cyclic nucleotide-activated channel CNGgust. J Biol Chem 272:22623-22629
26. Ohishi H, Akazawa C, Shigemoto R, Nakanishi S, Mizuno N (1995) Distributions of the mRNAs for L-2-amino-4-phosphonobutyrate-sensitive metabotropic glutamate receptors, mGluR4 and mGluR7, in the rat brain. J Comp Neurol 360:555-570
27. Olink-Coux M, Hollenbeck PJ (1996) Localization and active transport of mRNA in axons of sympathetic neurons in culture. J Neurosci 16:1346-1358
28. Panoskaltsis-Mortari A, Bucy RP (1995) In situ hybridization with digoxigenin-labeled RNA probes: facts and artifacts. BioTechniques 18:300-307
29. Prakash N, Fehr S, Mohr E, Richter D (1997) Dendritic localization of rat vasopressin mRNA: ultrastructural analysis and mapping of targeting elements. Eur J Neurosci 9:523-532
30. Raap AK, van de Corput MPC, Vervenne RAW, van Gijlswijk RP, Tanke HJ, Wiegant J (1995) Ultra-sensitive FISH using peroxidase-mediated deposition of biotin- or fluorochrome-ryramides. Hum Mol Genet 4:529-534
31. Ruiz-Avila L, McLaughlin SK, Wildman D, McKinnon PJ, Robichon A, Spickofsky N, Margolskee RF (1995) Coupling of bitter receptor to phosphodiesterase through transducin in taste receptor cells. Nature 376:80-85
32. Schaeren-Wiemers N, Gerfin-Moser A (1993) A single protocol to detect transcripts of various types and expression levels in neural tissue and cultured cells: in situ hybridization using digoxigenin-labeled cRNA probes. Histochemistry 100:431-440
33. Schmidt BF, Chao J, Zhu Z, DeBiasio R, Fisher G (1997) Signal amplification in the detection of single-copy DNA and RNA by enzyme-catalyzed deposition (CARD) of the novel fluorescent reporter substrate Cy3.29-tyramide. J Histochem Cytochem 45: 365-373
34. Schöfer C, Weipoltshammer K, Almeder M, Wachtler F (1997) Signal amplification at the ultrastructural level using biotinylated tyramides and immunogold detection. Histochem Cell Biol 108: 313-319
35. Singer RH, Lawrence JB, Villnave C (1986) Optimization of in situ hybridization using isotopic and non-isotopic detection methods. BioTechniques 4:230-250
36. Speel EJ, Ramaekers FC, Hopman AH (1997) Sensitive multicolor fluorescence in situ hybridization using catalyzed reporter deposition (CARD) amplification. J Histochem Cytochem 45:1439-1446
37. Tanahe Y, Masu M, Ishii T, Shigemoto R, Nakanishi S (1992) A family of metabotropic glutamate receptors. Neuron 8:169-179
38. Tanabe Y, Nomura A, Masu M, Shigemoto R, Mizuno N, Nakanishi S (1993) Signal transduction, pharmacological properties and expression patterns of two rat metabotropic glutamate receptors, mGluR3 and mGluR4. J Neurosci 13:1372-1378
39. Tanabe T, Nukada T, Nishikawa Y, Sugimoto K, Suzuki H, Takahashi H, Noda M, Haga T, Ichiyama A, Kangawa K (1985) Primary structure of the alpha-subunit of transducin and its relationship to ras proteins. Nature 315:242-245
40. van Gijlswijk RP, Zijlmans HJ, Wiegant J, Bobrow MN, Erickson TJ, Adler K, Tanke HJ, Raap AK (1997) Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization. J Histochem Cytochem 45:375-382
41. Wahle P, Beckh S (1992) A method of in situ hybridization combined with immunocytochemistry, histochemistry, and tract tracing to characterize the mRNA expressing cell types in heterogeneous neuronal populations. J Neurosci Methods 41:153-166
42. Wanner I, Baader SL, Brich M, Oberdick J, Schilling K (1997) Subcellular localization of specific mRNAs and their protein products in Purkinje cells by combined fluorescence in situ hybridization and immunocytochemistry. Histochem Cell Biol 108:345-357
43. Wilkinson DG, Nieto MA (1993) Detection of messenger RNA by in situ hybridization to tissue sections and whole mounts. Methods Enzymol 225:361-373
44. Wisden W, Morris BJ, Hunt SP (1991) in situ hybridization with synthetic DNA probes. In Chad J, Wheal H, eds. Molecular Neurobiology. Oxford, IRL Press, 205-225
45. Wong GT, Gannon KS, Margolskee RF (1996) Transduction of bitter and sweet taste by gustducin. Nature 381:796-800
46. Yamawaki M, Zurbriggen A, Richard A, Vandevelde M (1993) Saponin treatment for in situ hybridization maintains good morphological preservation. J Histochem Cytochem 41:105-109