Development of a microtiter plate fluorescent assay for inhibition studies on the HTLV-1 and HIV-1 proteinases

Journal of Virological Methods

Volumn 119, Issue 2, 2004, Pages 87-93

  Bagossi, Péter ^a   Kádas, János ^a   Miklóssy, Gabriella ^a   Boross, Péter ^a   Weber, Irene T ^b   Tözsér, József ^a  

^a UNIVERSITY OF DEBRECEN   (Hungary)

^b GEORGIA STATE UNIVERSITY   (United States)

Author keywords

CA; capsid protein; dimethyl sulfoxide; dithiothreitol; DMSO; DTT; HIV 1; HPLC; HTLV 1; human immunodeficiency virus type 1; human T cell leukemia virus type 1; MA; matrix protein; NC; nucleocapsid protein; PR; proteinase; TFA; trifluoroacetic acid

Indexed keywords

4,7 DIBENZYL 2,3,4,5,6,7 HEXAHYDRO 5,6 DIHYDROXY 1,3 BIS[4 (HYDROXYMETHYL)BENZYL] 2H 1,3 DIAZEPIN 2 ONE; AMPRENAVIR; INDINAVIR; NELFINAVIR; PEPTIDE DERIVATIVE; PROTEINASE; RITONAVIR; SAQUINAVIR; VIRUS PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CONCENTRATION RESPONSE; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN T CELL LEUKEMIA VIRUS 1; MICROTITER PLATE ASSAY; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DEGRADATION; VIRUS TITRATION;

ASPARTIC ENDOPEPTIDASES; ENZYME TESTS; FLUORESCENCE; HIV PROTEASE; HUMANS; INDINAVIR; KINETICS; PEPTIDES; SUBSTRATE SPECIFICITY;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN T-LYMPHOTROPIC VIRUS 1;

EID: 2442712423     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2004.03.001     Document Type: Article

References (23)

1. Ding Y.S., Rich D.H., Ikeda R.A. Substrates and inhibitors of human T-cell leukemia virus type I protease. Biochemistry. 37:1998;17514-17518
2. Gallo R.C. Human retroviruses after 20 years: a perspective from the past and prospects for their future control. Immunol. Rev. 185:2002;236-265
3. Grobelny D., Wondrak E.M., Galardy R.E., Oroszlan S. Selective phosphinate transition-state analogue inhibitors of the protease of human immunodeficiency virus. Biochem. Biophys. Res. Commun. 169:1990;1111-1116
4. Ha J.J., Gaul D.A., Mariani V.L., Ding Y.S., Ikeda R.A., Shuker S.B. HTLV-1 protease cleavage of p19/24 substrates is not dependent on NaCl concentration. Bioorg. Chem. 30:2002;138-144
5. Holskin B.P., Bukhtiyarova M., Dunn B.M., Baur P., de Chastonay J., Pennington M.W. A continuous fluorescence-based assay of human cytomegalovirus protease using a peptide substrate. Anal. Biochem. 227:1995;148-155
6. Hruskova-Heidingsfeldova O., Blaha I., Urban J., Strop P., Pichova I. Substrates and inhibitors of human T-cell leukemia virus type 1 (HTLV-1) proteinase. Leukemia. 11:1997;45-56
7. Johnson J.M., Harrod R., Franchini G. Molecular biology and pathogenesis of the human T-cell leukemia/lymphotropic virus type-1 (HTLV-1). Int. J. Exp. Pathol. 82:2001;135-147
8. Liu Y., Kati W., Chen C.M., Tripathi R., Molla A., Kohlbrenner W. Use of a fluorescence plate reader for measuring kinetic parameters with inner filter effect correction. Anal. Biochem. 267:1999;331-335
9. Louis J., McDonald R., Nashed N., Wondrak E.M., Jerina D., Oroszlan S., Mora P. Autoprocessing of the HIV-1 protease using purified wild-type and mutated fusion proteins expressed at high levels in Escherichia coli. Eur. J. Biochem. 199:1991;361-369
10. Louis J.M., Oroszlan S., Tözsér J. Stabilization from autoproteolysis and kinetic characterization of the human T-cell leukemia virus type 1 proteinase. J. Biol. Chem. 274:1999;6660-6666
11. Macchi B., Balestrieri E., Mastino A. Effects of nucleoside-based antiretroviral chemotherapy on human T cell leukaemia/lymphotropic virus type 1 (HTLV-1) infection in vitro. J. Antimicrob. Chemother. 51:2003;1327-1330
12. Mahalingam B., Louis J.M., Hung J., Harrison R.W., Weber I.T. Structural implications of drug-resistant mutants of HIV-1 protease: high-resolution crystal structures of the mutant protease/substrate analogue complexes. Proteins. 43:2001;455-464
13. Matayoshi E.D., Wang G.T., Kraft G.A., Erickson J. Novel fluorogenic substrates for assaying retroviral proteases by resonance energy transfer. Science. 247:1990;954-958
14. Oroszlan S., Luftig R.B. Retroviral proteinases. Curr. Top. Microbiol. Immunol. 157:1990;153-185
15. Pettit S.C., Sanchez R., Smith T., Wehbie R., Derse D., Swanstrom R. HIV type 1 protease inhibitors fail to inhibit HTLV-I Gag processing in infected cells. AIDS Res. Hum. Retroviruses. 14:1998;1007-1014
16. Sheremata W.A., Benedict D., Squilacote D.C., Sazant A., DeFreitas E. High-dose zidovudine induction in HTLV-I-associated myelopathy: safety and possible efficacy. Neurology. 43:1993;2125-2129
17. Swanstrom R., Eron J. Human immunodeficiency virus type-1 protease inhibitors: therapeutic success and failure, suppression and resistance. Pharmacol. Ther. 86:2000;145-170
18. Tözsér J., Blaha I., Copeland T.D., Wondrak E.M., Oroszlan S. Comparison of the HIV-1 and HIV-2 proteinases using oligopeptide substrates representing cleavage sites in Gag and Gag-Pol polyproteins. FEBS Lett. 281:1991;77-80
19. Tözsér J., Friedman D., Weber I.T., Blaha I., Oroszlan S. Studies on the substrate specificity of the proteinase of equine infectious anemia virus using oligopeptide substrates. Biochemistry. 32:1993;3347-3353
20. Tözsér J., Bagossi P., Weber I.T., Copeland T.D., Oroszlan S. Comparative studies on the substrate specificity of avian myeloblastosis virus proteinase and lentiviral proteinases. J. Biol. Chem. 271:1996;6781-6788
21. Tözsér J., Zahuczky G., Bagossi P., Louis J.M., Copeland T.D., Oroszlan S., Harrison R.W., Weber I.T. Comparison of the substrate specificity of the human T-cell leukemia virus and human immunodeficiency virus proteinases. Eur. J. Biochem. 267:2000;6287-6295
22. Wang G.T., Matayoshi E.D., Huffaker J., Krafft G.A. Design and synthesis of new fluorogenic HIV protease substrates based on resonance energy transfer. Tetrahedron Lett. 31:1990;6493-6496
23. Williams J.W., Morrison J.F. The kinetics of reversible tight-binding inhibition. Methods Enzymol. 63:1979;437-467