The crystal structure of the family 6 carbohydrate binding module from Cellvibrio mixtus endoglucanase 5A in complex with oligosaccharides reveals two distinct binding sites with different ligand specificities

Journal of Biological Chemistry

Volumn 279, Issue 20, 2004, Pages 21560-21568

  Pires, Virgínia M R ^a   Henshaw, Joanna L ^b   Prates, José A M ^a   Bolam, David N ^b   Ferreira, Luís M A ^a   Fontes, Carlos M G A ^a   Henrissat, Bernard ^c   Planas, Antoni ^d   Gilbert, Harry J ^b   Czjzek, Mirjam ^c  

^a FACULDADE DE MEDICINA VETERINÁRIA   (Portugal)

^b NEWCASTLE UNIVERSITY   (United Kingdom)

^c CNRS   (France)

^d INSTITUT QUÍMIC DE SARRIÀ   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

CARBOHYDRATES; CATALYSIS; CELLS; CRYSTAL STRUCTURE; OLIGOMERS; PLANTS (BOTANY); SUGARS;

CARBOHYDRATE BINDING MOLECULES (CBM); CATALYTIC DOMAINS; LIGANDS;

ENZYMES;

CARBOHYDRATE BINDING PROTEIN; CELLOBIOSE; GLUCAN SYNTHASE; GLUTAMIC ACID; GLYCOSIDASE; OLIGOSACCHARIDE; PROTEIN; PROTEIN CMCBM6; THREONINE; TRYPTOPHAN; TYROSINE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; BINDING SITE; CATALYSIS; CELLVIBRIO; CRYSTAL STRUCTURE; ENZYME SPECIFICITY; ENZYME SUBSTRATE; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN FAMILY; PROTEIN FUNCTION; PROTEIN STRUCTURE; SYNAPTIC TRANSMISSION;

BINDING SITES; CARBOHYDRATE CONFORMATION; CARBOHYDRATE SEQUENCE; CELLULASE; CELLVIBRIO; CRYSTALLOGRAPHY, X-RAY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; OLIGOSACCHARIDES; PROTEIN STRUCTURE, SECONDARY; SUBSTRATE SPECIFICITY;

ANIMALIA; CELLVIBRIO; CELLVIBRIO MIXTUS;

EID: 2442709187     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M401599200     Document Type: Article

References (29)

1. Brett, C. T., and Waldren, K. (1996) Physiology and Biochemistry of Plant Cell Walls, Chapman & Hall, London
2. Gilbert, H. J., Bolam, D. N., Szabo, L., Xie, H., Williamson, M. P., Simpson, P. J., Jamal, S., Boraston, A. B., Kilburn, D. G., and Warren, R. A. (2001) in Carbohydrate Bioengineering: Interdisciplinary Approaches (Teeri, T. T., Svensson, B., Gilbert, H. J., and Feizi, T., eds) Royal Society of Chemistry, Cambridge, UK
3. Gill, J., Rixon, J. E., Bolam, D. N., McQueen-Mason, S., Simpson, P. J., Williamson, M. P., Hazlewood, G. P., and Gilbert, H. J. (1999) Biochem. J. 342, 473-480
4. Lehtio, J., Sugiyama, J., Gustavsson, M., Fransson, L., Linder, M., and Teeri, T. T. (2003) Proc. Natl. Acad. Sci. U. S. A. 100, 484-489
5. Hogg, D., Pell, G., Dupree, P., Goubet, F., Martin-Orue, S. M., Armand, S., and Gilbert, H. J. (2003) Biochem. J. 371, 1027-1043
6. Boraston, A. B., Warren, R. A., and Kilburn, D. G. (2001) Biochemistry 40, 14679-14685
7. Abou Hachem, M., Nordberg Karlsson, E., Bartonek-Roxa, E., Raghothama, S., Simpson, P. J., Gilbert, H. J., Williamson, M. P., and Holst, O. (2000) Biochem. J. 345, 53-60
8. Tomme, P., Creagh, A. L., Kilburn, D. G., and Haynes, C. A. (1996) Biochemistry 35, 13885-13894
9. Zverlov, V. V., Volkov, I. Y., Velikodvorskaya, G. A., and Schwarz, W. H. (2001) Microbiology 147, 621-629
10. Henrissat, B., and Davies, G. J. (2000) Plant Physiol. 124, 1515-1519
11. Czjzek, M., Bolam, D. N., Mosbah, A., Allouch, J., Fontes, C. M., Ferreira, L. M., Bornet, O., Zamboni, V., Darbon, H., Smith, N. L., Black, G. W., Henrissat, B., and Gilbert, H. J. (2001) J. Biol. Chem. 276, 48580-48587
12. Boraston, A. B., Notenboom, V., Warren, R. A., Kilburn, D. G., Rose, D. R., and Davies, G. (2003) J. Mol. Biol. 327, 659-669
13. Charnock, S. J., Bolam, D. N., Turkenburg, J. P., Gilbert, H. J., Ferreira, L. M., Davies, G. J., and Fontes, C. M. (2000) Biochemistry 39, 5013-5021
14. Charnock, S. J., Bolam, D. N., Nurizzo, D., Szabo, L., McKie, V. A., Gilbert, H. J., and Davies, G. J. (2002) Proc. Natl. Acad. Sci. U. S. A. 99, 14077-14082
15. Boraston, A. B., Nurizzo, D., Notenboom, V., Ducros, V., Rose, D. R., Kilburn, D. G., and Davies, G. J. (2002) J. Mol. Biol. 319, 1143-1156
16. Notenboom, V., Boraston, A. B., Chiu, P., Freelove, A. C., Kilburn, D. G., and Rose, D. R. (2001) J. Mol. Biol. 314, 797-806
17. Powell, H. R. (1999) Acta Crystallogr. Sect. D 55, 1690-1695
18. Collaborative Computational Project No. 4 (1994) Acta Crystallogr. Sect. D 50, 760-763
19. Navaza, J. (1994) Acta Crystallogr. Sect. A 50, 157-163
20. Perrakis, A., Sixma, T. K., Wilson, K. S., and Lamzin, V. S. (1997) Acta Crystallogr. Sect. D 53, 448-455
21. Roussel, A., and Cambillau, C. (1991) in Silicon Graphics Geometry Partners Directory 88, Mountain View, CA
22. Szabo, L., Jamal, S., Xie, H., Charnock, S. J., Bolam, D. N., Gilbert, H. J., and Davies, G. J. (2001) J. Biol. Chem. 276, 49061-49065
23. Atkins, E. D. T. (1992) in Xylan and Xylanases: Progress in Biotechnology (Visser, J., Beldman, G., van Kusters, S., and Voragen, A. G. L., eds) Vol. 7, pp. 39-50, Elsevier Science Publishers B. V., Amsterdam
24. Simpson, P. J., Xie, H., Bolam, D. N., Gilbert, H. J., and Williamson, M. P. (2000) J. Biol. Chem. 275, 41137-41142
25. Tormo, J., Lamed, R., Chirino, A. J., Morag, E., Bayer, E. A., Shoham, Y., and Steitz, T. A. (1996) EMBO J. 15, 5739-5751
26. Brun, E., Johnson, P. E., Creagh, A. L., Tomme, P., Webster, P., Haynes, C. A., and McIntosh, L. P. (2000) Biochemistry 39, 2445-2458
27. Johnson, P. E., Brun, E., MacKenzie, L. F., Withers, S. G., and McIntosh, L. P. (1999) J. Mol. Biol. 287, 609-625
28. Pell, G., Szabo, L., Charnock, S. J., Xie, H., Gloster, T. M., Davies, G. J., and Gilbert, H. J. (2004) J. Biol. Chem. 279, 11777-11788
29. Henshaw, J., Bolam, D. N., Pires, V. M. R., Czjzek, M., Henrissat, B., Ferreira, L. M. A., Fontes, C. M. G. A., and Gilbert, H. J. (2003) J. Biol. Chem. 279, 21552-21559