Proteomic analysis of human cerebral cortex in epileptic patients

Experimental and Molecular Medicine

Volumn 36, Issue 2, 2004, Pages 185-191

  Eun, Jong Pil ^a   Choi, Ha Young ^a   Kwak, Yong Geun ^a  

^a Chonbuk National University Medical School   (South Korea)

Author keywords

Cerebral cortex; Epilepsy; Proteomics; Superoxide dismutase; Temporal lobe

Indexed keywords

CARBONATE DEHYDRATASE IV; CELL MARKER; DNA PRIMASE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; GLYCEROL 3 PHOSPHATE DEHYDROGENASE; MANGANESE SUPEROXIDE DISMUTASE; PROTEIN BAX; PROTEOME; TRYPSIN;

ADOLESCENT; ADULT; AGE; ARTICLE; BRAIN CORTEX; CHILD; CLINICAL ARTICLE; CONTROLLED STUDY; ENZYME ASSAY; EPILEPSY; GENDER; HUMAN; HUMAN TISSUE; NEOCORTEX; NUCLEOTIDE SEQUENCE; OXIDATIVE STRESS; PATHOGENESIS; PROTEOMICS; QUANTITATIVE ANALYSIS; SILVER STAINING; TEMPORAL LOBE EPILEPSY; TWO DIMENSIONAL ELECTROPHORESIS; WESTERN BLOTTING;

EID: 2442691786     PISSN: 12263613     EISSN: 20926413     Source Type: Journal    
DOI: 10.1038/emm.2004.26     Document Type: Article

References (21)

1. Armstead WM, Mirro R, Leffler CW, Busija DW. Cerebral superoxide anion generation during seizures in newborn pigs. J Cereb Blood Flow Metab 1989;9:175-9
2. Bellissimo MI, Amado D, Abdalla DS, Ferreira EC, Cavalheiro EA, Naffah-Mazzacoratti MG. Superoxide dismutase, glutathione peroxidase activities and the hydroperoxide concentration are modified in the hippocampus of epileptic rats. Epilepsy Res 2001;46:121-8
3. Ben-Menachem E, Kyllerman M, Marklund S. Superoxide dismutase and glutathione peroxidase function in progressive myoclonus epilepsies. Epilepsy Res 2000;40:33-9
4. Erakovic V, Zupan G, Varljen J, Radosevic S, Simonic A. Electroconvulsive shock in rats: changes in superoxide dismutase and glutathione peroxidase activity. Brain Res Mol Brain Res 2000;76:266-74
5. Halliwell B, Gutteridge JMC. Antioxidant defences. In Free Radicals in Biology and Medicine 1999a, 105-245, Oxford University Press, New York
6. Halliwell B, Gutteridge JMC. Free radicals, other reactive species and disease. In Free Radicals in Biology and Medicine 1999b, 617-783, Oxford University Press, New York
7. Hellman U, Wernstedt C, Gonez J, Heldin CH. Improvement of an "In-Gel" digestion procedure for the micropreparation of internal protein fragments for amino acid sequencing. Anal Biochem 1995;224:451-5
8. Kim YH, Lee JH, Lim do S, Shim WJ, Ro YM, Park GH, Becker KG, Cho-Chung YS, Kim MK. Gene expression profiling of oxidative stress on atrial fibrillation in humans. Exp Mol Med. 2003;35:336-49
9. Lai JCK, Clark JB. Preparation of synaptic and nonsynaptic mitochondria from mammalian brain. In Methods in Enzymology (Fleischer S, Packer L. eds), 1979, 51-60, Academic Press, New York, NY
10. Liang LP, Ho YS, Patel M. Mitochondrial superoxide production in kainate-induced hippocampal damage. Neuroscience 2000;101:563-70
11. Link WA, Kauselmann G, Mellstrom B, Kuhl D, Naranjo JR. Induction of glycerol phosphate dehydrogenase gene expression during seizure and analgesia. J Neurochem 2000;75:1419-28
12. Meisler MH, Kearney J, Ottman R, Escayg A. Identification of epilepsy genes in human and mouse. Annu Rev Genet 2001;35:567-88
13. Mori N, Yokoyama H. Role of superoxide dismutase in a kindling model of epilepsy. Comp Biochem Physiol C 1993;104:373-6
14. Murashima YL, Kasamo K, Suzuki J. Antiepileptic effects of allopurinol on EL mice are associated with changes in SOD isoenzyme activities. Epilepsy Res 1998;32:254-65
15. Nebot C, Moutet M, Huet P, Xu JZ, Yadan JC, Chaudiere J. Spectrophotometric assay of superoxide dismutase activity based on the activated autoxidation of a tetracyclic catechol. Anal Biochem 1993;214:442-51
16. Oliver CN, Starke-Reed PE, Stadtman ER, Liu GJ, Carney JM, Floyd RA. Oxidative damage to brain proteins, loss of glutamine synthetase activity, and production of free radicals during ischemia/reperfusion- induced injury to gerbil brain. Proc Natl Acad Sci USA 1990;87:5144-7
17. Park SA, Kim TS, Choi KS, Park HJ, Heo K, Lee Bi. Chronic activation of CREB and p90RSK in human epileptic hippocampus. Exp Mol Med. 2003;35:365-70
18. Patel MN. Oxidative stress, mitochondrial dysfunction, and epilepsy. Free Radic Res 2002;36:1139-46
19. Scheler C, Lamer S, Pan Z, Li XP, Salnikow J, Jungblut P. Peptide mass fingerprint sequence coverage from differently stained proteins on two-dimensional electrophoresis patterns by matrix assisted laser desorption/ionization-mass spectrometry (MALDI-MS). Electrophoresis 1998;19:918-27
20. Shevchenko A, Wilm M, Vorm O, Mann M. Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal Chem 1996;68:850-8
21. Stefan H, Halasz P, Gil-Nagel A, Shorvon S, Bauer G, Ben-Menachem E, Perucca E, Wieser HG, Steinlein O. Recent advances in the diagnosis and treatment of epilepsy. Eur J Neurol 2001;8:519-39