Native chemical ligation of hydropobic peptides in lipid bilayer systems

Bioconjugate Chemistry

Volumn 15, Issue 3, 2004, Pages 437-440

  Hunter, Christie L ^a,b   Kochendoerfer, Gerd G ^a  

^a Gryphon Therapeutics   (United States)

^b APPLIED BIOSYSTEMS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CHEMICAL MODIFICATION; PEPTIDES; POLYPEPTIDES;

BILAYER SYSTEMS; COVALENT MODIFICATIONS; FOLDED MEMBRANES; HYDROPHOBICS; KEY FEATURE; MEMBRANE PROTEINS; N-TERMINALS; NATIVE CHEMICAL LIGATION; PHASE MATRIX; REACTION MEDIA;

LIPID BILAYERS;

AMINO ACID; AQUAPORIN; CYSTEINE; DETERGENT; MEMBRANE PROTEIN; PEPTIDE; POLYPEPTIDE; THIOESTER;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CHEMICAL LIGATION REACTION; CHEMICAL REACTION; CUBIC LIPIDIC PHASE; HYDROPHOBICITY; LIPID BILAYER; LIQUID; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MEMBRANE STRUCTURE; PROTEIN DEGRADATION; PROTEIN DENATURATION; PROTEIN FOLDING; REACTION ANALYSIS; SOLUBILIZATION;

EID: 2442643066     PISSN: 10431802     EISSN: None     Source Type: Journal    
DOI: 10.1021/bc049959s     Document Type: Article

References (19)

1. Kochendoerfer, G. G., Salom, D., Lear, J. D., Wilk-Orescan, R., Kent, S. B. H., and DeGrado, W. F. (1999) Total Chemical Synthesis of the Integral Membrane Protein Influenza A Virus M2: Role of Its C-Terminal Domain in Tetramer Assembly. Biochemistry 38, 11905-11913.
2. Clayton, D., Shapovalov, G., Maurer, J. H., Dougherty, D. A., Lester, H. A., and Kochendoerfer, G. G. (2004) Total chemical synthesis and electrophysiological characterization of mechanosensitive channels from Escherichia coli and Mycobacterium tuberculosis. Proc. Natl. Acad. Sci. 101, 4764-4769.
3. Kochendoerfer, G., Jones, D., Lee, S., Oblatt-Montal, M., Opella, S., and Montal, M. (2004) Functional characterization and NMR spectroscopy on full-length Vpu from HIV-1 prepared by total chemical synthesis. J. Am. Chem. Soc. 137, 2439-2446.
4. Schnölzer, M., and Kent, S. B. H. (1992) Constructing Proteins by Dovetailing Unprotected Synthetic Peptides: Backbone-Engineered HIV Protease. Science 256, 221-225.
5. Dawson, P. E., Muir, T. W., Clark-Lewis, I., and Kent, S. B. (1994) Synthesis of proteins by native chemical ligation. Science 266, 776-779.
6. Dawson, P. E., and Kent, S. B. H. (2000) Synthesis of native proteins by chemical ligation. Annu. Rev. Biochem 69, 923-962.
7. Kochendoerfer, G. G. (2001) Chemical protein synthesis methods in drug discovery. Curr. Opin. Drug. Discovery Dev. 4, 205-214.
8. Muir, T. W. (2003) Semisynthesis of proteins by expressed protein ligation. Annu. Rev. Biochem. 72, 249-289.
9. Muir, T. W., Sondhi, D., and Cole, P. A. (1998) Expressed protein ligation: a general method for protein engineering. Proc. Natl. Acad. Sci. U.S.A. 95, 6705-6710.
10. Xu, R., Ayers, B., Cowburn, D., and Muir, T. W. (1999) Chemical ligation of folded recombinant proteins: segmental isotopic labeling of domains for NMR studies. Proc. Natl. Acad. Sci. U.S.A. 96, 388-393.
11. Erlanson, D. A., Chytil, M., and Verdine, G. L. (1996) The leucine zipper domain controls the orientation of AP-1 in the NFAT.AP-1.DNA complex. Chem. Biol. 3, 981-991.
12. Valiyaveetil, F. I., MacKinnon, R., and Muir, T. W. (2002) Semisynthesis and folding of the potassium channel KcsA. J. Am. Chem. Soc. 124, 9113-9120.
13. Luzzatti, V., Gulik-Krzywicki, T., and Tardieu, A. (1968) Polymorphism of lecithins. Nature 218, 1031-1034.
14. Lindblom, G., and Rilfors, L. (1989) Cubic phases and isotropic structures formed by membrane lipids-possible biological relevance. Biochim. Biophys. Acta 988, 221-256.
15. Rowinski, P., Korytkowska, A., and Bilewicz, R. (2003) Diffusion of hydrophilic probes in bicontinuous lipidic cubic phase. Chem. Phys. Lip. 124, 147-156.
16. Portmann, M., Landau, E. M., and Luisi, P. L. (1991) Spectroscopic and Rheological Studies of Enzymes in Rigid Lipidic Matrixes: The Case of α-Chymotrypsin in a Lysolecithin/Water cubic Phase. J. Phys. Chem. 95, 8437-8440.
17. + channel. Biochemistry 41, 10771-10777.
18. Perler, F. B., Xu, M. Q., and Paulus, H. (1997) Protein splicing and autoproteolysis mechanisms. Curr. Opin. Chem. Biol. 1, 292-299.
19. Hunt, J. F., Bousche, O., Meyers, K. M., Rothschild, K. J., and Engelman, D. M. (1991) Biophysical studies of the integral membrane protein folding pathway. Biophys. J. 59, 400a.