One-step purification of poly-his tagged penicillin G acylase expressed in E. coli

Journal of Microbiology and Biotechnology

Volumn 14, Issue 2, 2004, Pages 231-236

  Kim, Jin Hee ^a   Kang, Hye Jin ^a   Kim, Eung Soo ^a   Kim, Jeong Ho ^a   Koo, Yoon Mo ^a  

^a Inha University   (South Korea)

Author keywords

E. coli ATCC 11105; IMAC; One step purification; Osmotic shock; Penicillin G acylase

Indexed keywords

BETA GALACTOSIDASE; CELL EXTRACT; HISTIDINE; PENICILLIN AMIDASE; PENICILLIN G;

ARTICLE; BACTERIAL GENE; BACTERIUM CULTURE; BETA CHAIN; CARBOXY TERMINAL SEQUENCE; CELL FRACTIONATION; CODON; CONTROLLED STUDY; CYTOPLASM; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME SUBUNIT; ENZYME SYNTHESIS; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE FUSION; GENETIC CODE; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; INTERMETHOD COMPARISON; MOLECULAR SIZE; NONHUMAN; OSMOTIC STRESS; POLYMERASE CHAIN REACTION; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN SECRETION; SEQUENCE TAGGED SITE; TEMPERATURE;

EID: 2442623400     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. Armisen, P., C. Mateo, E. Cortes, J. L. Barredo, F. Salto, B. Diez, L. Rodes, J. L. Garcia, R. Fernandez-Lafuente, and J. M. Guisan. 1999. Selective adsorption of poly-His tagged glutaryl acylase on tailor-made metal chelate supports. J. Chromatogr. A 848: 61-70.
2. Balasingham, K., D. Warburton, P. Dunnill, and M. D. Lilly. 1972. The isolation and kinetics of penicillin amidase from Escherichia coli. Biochim. Biophys. Acta 276: 250-256.
3. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
4. Burtscher, H. and G. Schumacher. 1992. Reconstitution in vivo of penicillin G acylase activity from separately expressed subunits. Eur. J. Biochem. 205: 77-83.
5. Chae, Y. K., W. C. Jeon, and K. S. Cho. 2002. Rapid and simple method to prepare functional Pfu DNA polymerase expression in Escherichia coli periplasm. J. Microbiol. Biotechnol. 12: 841-843.
6. Erarslam, A. 1995. The effect of polyol compounds on the thermostability of penicillin G acylase from a mutant of Escherichia coli ATCC 11105. Proc. Biochem. 30: 133-139.
7. Erarslan, A., I. Terzi, A. Guray, and E. Bermek. 1991. Purification and kinetics of penicillin G acylase from a mutant strain of Escherichia coli ATCC 11105. J. Chem. Technol. Biotechnol. 51: 27-40.
8. Fitton, V. and X. Santareli. 2001. Evaluation of immobilized metal affinity chromatography for purification of penicillin acylase. J. Chromatogr. B 754: 135-140.
9. Fitton, V., N. Verdoni, J. Sanchez, and X. Santarelli. 2001. Penicillin acylase purification with the aid of pseudo-affinity chromatography. J. Biochem. Biophys. Methods 49: 553-560.
10. Gibert, S., N. Bakalara, and X. Santarelli. 2000. Three-step chromatographic purification procedure for the production of a His-tag recombinant kinesin overexpressed in E. coli. J. Chromatogr. B 737: 143-150.
11. Guan, Y. H., T. H. Lilley, and A. H. Brook. 2001. Production of immobilized penicillin acylase using aqueous polymer systems for enzyme purification and in situ immobilization. Enzyme Microb. Technol. 28: 218-224.
12. Gumusel, F., S. I. Ozturk, N. K. Korkut, C. Gelegen, and E. Bermek. 2001. Cloning and expression of the penicillin acylase gene (pac) from E. coli ATCC 11105. Enzyme Microb. Technol. 29: 499-505.
13. Kazan, D. and A. Erarslan. 1996. Influence of polyhydric compounds on the pH stability of penicillin G acylase obtained from a mutant of Escherichia coli ATCC 11105. Proc. Biochem. 31: 691-697.
14. Kazan, D. and A. Erarslan. Identification of catalytically essential amino acid residues of penicillin G acylase obtained from a mutant of Escherichia coli ATCC 11105. Proc. Biochem. 36: 861-867.
15. Kheirolomoom, A., M. Ardjmand, H. Fazelinia, and A. Zakeri. 2001. Isolation of penicillin G acylase from Escherichia coli ATCC 11105 by physical and chemical treatments. Biochem. Eng. J. 8: 223-227.
16. Kheirolomoom, A., M. Ardjmand, H. Fazelinia, and A. Zakeri. 2001. Clarification of penicillin G acylase reaction mechanism. Proc. Biochem. 36: 1095-1101.
17. Martin, L., M. A. Prieto, E. Cortes, and J. L. Garcia. 1995. Cloning and sequencing of the pac gene encoding the penicillin G acylase of Bacillus megaterium ATCC 14945. FEMS Microbiol. Lett. 125: 287-292.
18. Kim, D. W., S.-M. Kang, and K. H. Yoon. 2001. Characterization of glutaryl 7-ACA acylase from pseudomonas diminuta KAC-1. J. Microbiol. Biotechnol. 11: 452-457.
19. Oh, S.-J., Y.-C. Kim, Y.-W. Park, S.-Y. Min, I.-S. Kim, and H.-S. Kang. 1987. Complete nucleotide sequence of the penicillin G acylase gene and the flanking regions and its expression in Escherichia coli. Gene 56: 87-97.
20. Robas, N., H. Zouheiry, G. Branlant, and C. Branlant. 1993. Improved penicillin amidase production using a genetically engineered mutant of Escherichia coli ATCC 11105. Biotechnology and Bioengineering 41: 14-24.
21. Sambrook, J., E. F. Fritch, and T. Maniatis. 1989. Molecular cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, U.S.A.
22. Sanchez, J., N. Verdoni, V. Fitton, and X. Santarelli. 2001. Efficient two-step chromatographic purification of penicillin acylase from clarified Escherichia coli ultrasonic homogenate. J. Chromatogr. B 753: 45-50.
23. Sapan, C. V., R. L. Lundblad, and N. C. Price. 1999. Colorimetric protein assay techniques. Biotechnol. Appl. Biochem. 29: 99-108.
24. Schumacher, G., D. Sizmann, H. Haug, P. Buckel, and A. Bock. 1986. Penicillin G acylase from E. coli: Unique gene-protein relation. Nucl. Acids Res. 14: 5713-5727.
25. Vohra, P. K., R. Sharma, D. R. Kashyap, and R. Tewari. 2001. Enhanced production of penicillin G acylase from a recombinant Escherichia coli. Biotech. Lett. 23: 531-535.