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Bioorganic and Medicinal Chemistry Letters
Volumn 14, Issue 12, 2004, Pages 3109-3112
Corrigendum to "Human ACAT-1 and -2 inhibitory activities of saucerneol B, manassantin A and B isolated from Saururus chinensis" [Bioorg. Med. Chem. Lett. 14 (2004) 3109-3112] (DOI:10.1016/j.bmcl.2004.04.023);Human ACAT-1 and -2 inhibitory activities of saucerneol B, manassantin A and B isolated from Saururus chinensis
Author keywords

hACAT 1 and hACAT 2; Hypocholesterolemic activity; Inhibitor; Lignans; S. chinensis
Indexed keywords

MANASSANTIN A; MANASSANTIN B; METHANOL DERIVATIVE; PLANT EXTRACT; SAUCERNEOL B; UNCLASSIFIED DRUG;
EID: 2442541099     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2004.04.108     Document Type: Erratum
Times cited : (45)
References (28)
  • 23
    • 2442501139 scopus 로고    scopus 로고
    • note
    • 14C] oleoyl-CoA solution (0.05 μCi, final concn 10 μM). After 25 min of incubation at 37°C, the reaction was stopped by the addition of 1.0 mL of isopropanol-heptane (4:1; v/v) solution. A mixture of 0.6 mL of heptane and 0.4 mL of 0.1 M potassium-phosphate buffer (pH 7.4) with 2 mM dithiothreitol was then added to the terminated reaction mixture. The above solution was mixed and allowed to phase separation under gravity for 2 min. Cholesterol oleate was recovered in the upper heptane phase (total volume 0.9-1.0 mL). The radioactivity in 100 μL of the upper phase was measured in a 3 mL liquid scintillation viral with 3 mL of scintillation cocktail (Lipoluma, Lumac Co.) using a liquid scintillation counter (1450 Micerobeta Trilux Wallac Oy, Turku, Finland). Background values were obtained by preparing heat inactivated microsomes or normal insect cell lysate microsomes, usually background value was 200-250 cpm, while 8000 cpm of ACAT reaction. The ACAT activity was expressed as a defined unit, cholesteryl oleate pmol/min/mg protein
  • 24
    • 2442587208 scopus 로고    scopus 로고
    • note
    • The hypocholesterolemic effect of the EtOAc-soluble fraction of MeOH extract of S. chinensis was investigated in male C57BL/6J mice maintained at Korea Research Institute of Bioscience and Biotechnology (Daejeon, Korea). The mice were housed in a room with controlled temperature (22 ± 2°C), relative humidity (55 ± 5%), and lighting (alternating 12 h cycle of light and dark). At 7 weeks of age, 30 male mice were randomly divided into three groups of 10 animals and fed on a high cholesterol diet (CRF-1 supplemented with 15% fat, 1.25% cholesterol, and 0.5% Na-cholate, Oriental Yeast Co. Ltd, Japan), the first group without supplementation (control), the second group supplemented with 0.5% (wt/wt diet) of the EtOAc-soluble fraction of S. chinensis, and third group supplemented with 0.1% (wt/wt diet) of probucol (positive control). Probucol was purchased from Sigma Chemical Co. Ltd. The diet and water were given ad libitum. After treating the test compounds for 10 days, the mice were anesthetized with ethyl ether, and the blood was obtained from the retro-orbital sinus using a heparinized capillary tube. Then, the blood was centrifuged at 8000g for 10 min, and the plasma was collected. The concentration of plasma total cholesterol was measured with an automatic blood chemical analyzer (Hitachi 7020, Japan). To evaluate statistical significance between control and experimental groups, student's t -test was performed, and a p value of <0.05 was considered to be statistically significant

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