Radiosensitization mechanism of riboflavin in vitro

Science in China, Series C: Life Sciences

Volumn 45, Issue 4, 2002, Pages 344-352

  Liu, Guanshu ^a   Lu, Changyuan ^a   Yao, Side ^a   Zhao, Fang ^b   Li, Yu ^b   Meng, Xiangshun ^b   Gao, Jianguo ^b   Cai, Jianming ^b   Zhang, Liming ^b   Chen, Zhilong ^b  

^a INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^b SECOND MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

Fluorescence microscopy; L02 cell; MTT method; Murine thymocyte; Radiosensitization; Riboflavin

Indexed keywords

CELL MEMBRANES; CONCENTRATION (PROCESS); CYTOLOGY; DNA; FLUORESCENCE; IRRADIATION;

FLUORESCENCE MICROSCOPY; LO2 CELLS; MTT METHOD; MURINE THYMOCYTE; RADIOSENSITIZATION; RIBOFLAVIN;

VITAMINS;

MURINAE;

EID: 2442445308     PISSN: 10069305     EISSN: None     Source Type: Journal    
DOI: 10.1360/02yc9038     Document Type: Article

References (16)

1. Bensasson, R. V., Land, E. J., Truscott, T. G., Flash Photolysis and Pulse Radiolysis in Biology and Medicine, 1st ed., Oxford: Pergamon Press, 1983, 140-147.
2. Heelis, P. F., The photophysical and photochemical properties of flavins (isoalloxazines), Chem. Soc. Rev., 1982, 11: 15-39.
3. Ito, K., Inoue, S., Yamamoto, K. et al., 8-Hydroxyguanosine formation at the 5′ site of 5-GG-3′ sequences in double-stranded DNA by UV radiation with riboflavin, J. Biol. Chem., 1993, 269: 13221-13227.
4. Lu, C. Y., Wang, W. F., Lin, W. Z. et al., Generation and photosensitization properties of the oxidized radical of riboflavin: a laser flash photolysis study, J. Photochem. Photobiol., B: Biology, 1999, 52: 111-116.
5. 2)-sensitized photooxidation of 2′-deoxyguanosine-5′- monophosphate (dGMP) in aqueous solution: a transient intermediates study, Phys. Chem. Chem. Phys., 2000, 2: 329-335.
6. 2): potential photobiological effects, Radiat. Res. Radiat. Process (in Chinese), 2002, 2: 12-18.
7. Lu, C. Y., Yao, S. D., Lin, W. Z. et al., Kinetic observation of rapid electron transfer between pyrimidine electron adducts and sensitizers of riboflavin, flavin adenine dinucleotide (FAD) and chloranil: a pulse radiolysis study, Radiat. Phys. Chem., 2000, 59: 61-66.
8. Lu, C. Y., Han, Z. H., Tu, T. C. et al., Kinetic observation of rapid electron transfer from electron adducts and reducing OH adducts of dAMP to riboflavin, flavin adenine dinucleotide (FAD): a pulse radiolysis study, Chin. J. Chem., 2000, 18: 500-506.
9. Lu, C. Y., Yao, S. D., Han, Z. H. et al., Reaction of reducing hydroxyl radical adducts of pyrimidine nucleotides with riboflavin and flavin adenine dinucleotide (FAD) via electron transfer: A pulse radiolysis study, Biophys. Chem., 2000, 85: 17-24.
10. Hamind, M. S. Alvaro, O., Thomas, Y. M. et al., Riboflavin uptake by the human-derived hepatoma cells Hep G2: mechanism and regulation, J. Cell Physiol., 1998, 176: 588-594.
11. Eble, M. J., Hensley, F. W., Flentje, M. et al., A modified computer-assisted colorimetric microtitre assay (MTT) to assess in vitro radiosensitivity of V79, CaSki, Hela, and WiDr cells, Int. J. Radiat. Biol., 1994, 65: 193-201.
12. Situ, Z. Q., Wu, J. Z., Cell Culture (in Chinese), Xi'an: World Books Press Co., 1996, 186-187.
13. Gederaas, O. A., Rasch, M. H., Berg, K. et al., Photodynamically induced effects in colon carcinoma cells (WiDr) by endogenous photosensitizers generated by incubation with 5-aminolaevulinic acid, J. Photochem. Photobiol., B: Biol., 1999, 49: 162-170.
14. Melnikova, V. O., Bezdetnaya, L. N., Bour, C. et al., Subcellular localization of meta (hydroxyphenyl)-chlorin in human tumor cell subjected to photodynamic treatment, J. Photochem. Photobiol., B: Biol., 1999, 49: 96-103.
15. Xia, S. X., Liu, S. Z., Molecular Radiation Biology (in Chinese), Beijing: Nuclear Energy Press, 1992, 342.
16. Kotaki, Y., Yagi, K., Fluorescence properties of flavins in various solutions, J. Biochem., 1970, 68: 509-516.