Structure-Function Relationship of the Influenza Virus RNA Polymerase: Primer-Binding Site on the PB1 Subunit

Biochemistry

Volumn 43, Issue 19, 2004, Pages 5882-5887

  Kolpashchikov, Dmitry M ^a,d   Honda, Ayae ^b,c   Ishihama, Akira ^a,b  

^a NATIONAL INSTITUTE OF GENETICS   (Japan)

^b NIPPON INSTITUTE FOR BIOLOGICAL SCIENCE   (Japan)

^c NIHON UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^d Och Spine at New York Presbyterian Hospitals   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; CROSSLINKING; ENZYMES; GENES; POLYMERIZATION; RNA; VIRUSES;

BINDING SITES; PROTEOLYSIS; STRUCTURE-FUNCTION RELATIONSHIP; TRANSCRIPTION;

BIOCHEMISTRY;

ADENOSINE TRIPHOSPHATE; DINUCLEOTIDE; GUANOSINE TRIPHOSPHATE; NUCLEOTIDE; PHOSPHATE; PRIMER RNA; PROTEIN PA; PROTEIN PB1; PROTEIN PB2; PROTEIN SUBUNIT; PROTEINASE; RNA POLYMERASE; UNCLASSIFIED DRUG; V8 PROTEASE; VIRUS PROTEIN; VIRUS RNA;

ARTICLE; BINDING SITE; CROSS LINKING; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME STRUCTURE; ENZYME SUBSTRATE; GENETIC TRANSCRIPTION; INFLUENZA VIRUS; NONHUMAN; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN FUNCTION; STRUCTURE ANALYSIS; VIRUS GENOME; VIRUS REPLICATION;

BINDING SITES; CATALYSIS; CODON, INITIATOR; CROSS-LINKING REAGENTS; CYCLIC AMP; DNA PRIMERS; DNA-DIRECTED RNA POLYMERASES; HYDROLYSIS; ORTHOMYXOVIRIDAE; PEPTIDE CHAIN ELONGATION, TRANSLATIONAL; PROTEIN SUBUNITS; RIBONUCLEOPROTEINS; RNA, VIRAL; SERINE ENDOPEPTIDASES; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; VIRAL PROTEINS;

INFLUENZA VIRUS; RNA VIRUSES;

EID: 2442444487     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi036139e     Document Type: Article

References (25)

1. Honda, A., and Ishihama, A. (1997) The molecular anatomy of influenza virus RNA polymerase, Biol. Chem. 378, 483-488.
2. Ishihama, A., and Barbier, P. (1994) Molecular anatomy of viral RNA-directed RNA polymerase, Arch. Virol. 134, 235-258.
3. Honda, A., Mukaigawa, J., Yokoiyama, A., Kato, A., Ueda, S., Nagata, K., Krystal, M., Nayak, D., and Ishihama, A. (1990) Purification and molecular structure of RNA polymerase from influenza virus A/PR8, J. Biochem. 107, 624-628.
4. Krug, R. (1981) Priming of influenza virus RNA transcription by capped heterologous RNAs, Curr. Top. Microbiol. Immunol. 93, 125-149.
5. Honda, A., Mizumoto, K., and Ishihama, A. (1998) A simple enzymatic method for analysis of RNA 5′-terminal structures: Quantitative determination of the 5′ termini of influenza virus genome RNAs, Virus Res. 55, 199-206.
6. Asano, Y., Mizumoto, K., Maruyama, T., and Ishihama, A. (1995) Photoaffinity labeling of influenza virus RNA polymerase PB1 subunit with 8-azido GTP, J. Biochem. 117, 677-82.
7. Braam, J., Ulmanen, I., and Krug, R. M. (1983) Molecular model of a eucaryotic transcription complex: functions and movements of influenza P proteins during capped RNA-primed transcription, Cell 34, 609-618.
8. Poch, O., Sauvaget, I., Delarue, M., and Tordo, N. (1999) Identification of four conserved motifs among the RNA-dependent polymerase encoding elements, EMBO J. 8, 3867-3874.
9. Biswas, S. K., and Nayak, D. P. (1994) Mutational analysis of the conserved motifs of influenza A virus polymerase basic protein 1, J. Virol. 68, 1819-1826.
10. Kobayashi, M., Toyoda, T., and Ishihama, A. (1996) Influenza PB1 protein is the minimal and essential subunit of RNA polymerase, Arch. Virol. 141, 525-539.
11. Nakagawa, Y., Oda, K., and Nakada, S. (1996) The PB1 subunit alone can catalyze cRNA synthesis, and the PA subunit in addition to the PB1 subunit is required for viral RNA synthesis in replication of the influenza genome, J. Virol. 70, 6390-6394.
12. Li, M. L., Rao, P., and Krug, R. M. (2001) The active sites of the influenza cap-dependent endonuclease are on different polymerase subunit, EMBO J. 17, 2078-2085.
13. Honda, A., Mizumoto, K., and Ishihama, A. (1999) Two separate sequences of PB2 subunit constitute the RNA cap-binding site of influenza virus RNA polymerase, Genes Cells 4, 475-485.
14. Asano, Y., and Ishihama, A. (1997) Identification of two nucleotide-biding domains on the PB1 subunit of influenza virus RNA polymerase, J. Biochem. 122, 627-634.
15. Grachev, M. A., Kolocheva, T. I., Lukhtanov, E. A., and Mustaev, A. A. (1987) Studies on the functional topography of Escherichia coli RNA polymerase. Highly selective affinity labeling by analogues of initiating substrates, Eur. J. Biochem. 163, 113-121.
16. Grachev, M. A., Lukhtanov, E. A., Mustaev, A. A., Zaychikov, E. F., Abdukayumov, M. N., Rabinov, I. V., Richter, V. I., Skoblov, Y. S., and Chistyakov, P. G. (1989) Studies of the functional topography of Escherichia coli RNA polymerase. A method for localization of the sites of affinity labeling, Eur. J. Biochem. 180, 577-585.
17. Knorre, D. G., Kurbatov, V. A., and Samukov, V. V. (1976) General method for the synthesis of ATP γ derivatives, FEBS Lett. 70, 105-108.
18. Morozova, O. V., Mustaev, A. A., Belyavskaya, N. A., Zaychikov, E. F., Kvetkova, E. A., Wolf, Y., and Pletnev, A. G. (1990) Mapping of the region of the tick-borne encephalitis virus replicase adjacent to initiating substrate binding center, FEBS Lett. 277, 75-77.
19. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680-685.
20. Godovikova, T. S., Grachev, M. A., Kutyavin, I. V., Tsarev, I. G., Zarytova, V. F., and Zaychikov, E. F. (1987) Studies of the functional topography of Escherichia coli RNA polymerase. Affinity labeling of RNA polymerase in a promoter complex by phosphorylating derivatives of primer oligonucleotides, Eur. J. Biochem. 166, 611-616.
21. Honda, A., Endo, A., Mizumoto, K., and Ishihama, A. (2001) Differential roles of vRNA and cRNA in functional modulation of the influenza virus RNA polymerase, J. Biol. Chem. 276, 31179-31185.
22. Honda, A., Mizumoto, K., and Ishihama, A. (2002). Minimum molecular architectures for transcription and replication of the influenza virus, Proc. Natl. Acad. Sci. U.S.A. 99, 13166-13171.
23. Honda, A., Mizumoto, K., and Ishihama, A. (1986) RNA polymerase of influenza virus. Dinucleotide-primed initiation of transcription at specific positions on viral RNA, J. Biol. Chem. 261, 5987-5991.
24. Krummel, B., and Chamberlin, M. J. (1992) Structural analysis of ternary complexes of Escherichia coli RNA polymerase. Individual complexes halted along different transcription units have distinct and unexpected biochemical properties, J. Mol. Biol. 225, 221-237.
25. Krummel, B., and Chamberlin, M. J. (1989) RNA chain initiation by Escherichia coli RNA polymerase. Structural transitions of the enzyme in early ternary complexes, Biochemistry 28, 7829-7842.