Identification of two nucleotide-binding domains on the PB1 subunit of influenza virus RNA polymerase

Journal of Biochemistry

Volumn 122, Issue 3, 1997, Pages 627-634

  Asano, Yukiyasu ^a,b   Ishihama, Akira ^a  

^a NATIONAL INSTITUTE OF GENETICS   (Japan)

^b Rational Drug Design Laboratories   (Japan)

Author keywords

Cross linking; Influenza virus; RNA polymerase; Substrate binding

Indexed keywords

GUANOSINE TRIPHOSPHATE; NUCLEOTIDE BINDING PROTEIN; PROTEINASE; RNA POLYMERASE; VIRUS ENZYME;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; ENZYME ACTIVE SITE; GENETIC CONSERVATION; INFLUENZA VIRUS; NONHUMAN; PROTEIN CROSS LINKING; PROTEIN DOMAIN; PROTEIN STRUCTURE;

EID: 0030744812     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021799     Document Type: Article

References (31)

1. Ishihama, A. and Nagata, K. (1988) Viral RNA polymerase. CRC Crit. Rev. Biochem. 23, 27-76
2. Krug, R.M., Alonso-Caplen, F.V., Julkunen, I., and Katze, M.G. (1989) Expression and replication of the influenza virus genome in The Influenza Viruses (Krug, R.M., ed.) pp. 89-152, Plenum Press, New York
3. Ishihama, A. and Barbier, P. (1994) Molecular anatomy of viral RNA-directed RNA polymerases. Arch. Virol. 134, 235-258
4. Honda A., Mukaigawa, J., Yokoiyama, A., Kato, A., Ueda, S., Nagata, K., Krystal, M., Nayak, D.P., and Ishihama, A. (1990) Purification and molecular structure of RNA polymerase from influenza virus A/PR8. J. Biochem. 107, 624-628
5. Ulmanen, I., Broni, B.A., and Krug, R.M. (1981) Role of two of the influenza virus core P proteins in recognizing cap 1 structures (m7GpppNm) on RNAs and in initiating viral RNA transcription. Proc. Natl. Acad. Sci. USA 78, 7355-7359
6. Blaas, D., Patzelt, E., and Kuechler, E. (1982) Identification of the cap binding protein of influenza virus. Nucleic Acids Res. 10, 4803-4812
7. Ulmanen, I., Broni, B., and Krug, R.M. (1983) Influenza virus temperature-sensitive cap (m7GpppNm)-dependent endonuclease. J. Virol. 45, 27-35
8. Asano, Y., Mizumoto, K., Maruyama, T., and Ishihama, A. (1995) Photoaffinity labeling of influenza virus RNA polymerase PB1 subunit with 8-azido GTP. J. Biochem. 117, 677-682
9. Braam, J., Ulmanen, I., and Krug, R.M. (1983) Molecular model of a eucaryotic transcription complex: functions and movements of influenza P proteins during capped RNA-primed transcription. Cell 34, 609-618
10. Fodor, E., Seong, B.L., and Brownlee, G.G. (1993) Photochemical cross-linking of influenza A polymerase to its virion RNA promoter defines a polymerase binding site at residues 9 to 12 of the promoter. J. Gen. Virol. 74, 1327-1333
11. Toyoda, T., Adyshev, D., Kobayashi, M., Iwata, A., and Ishihama, A. (1996) Molecular assembly of influenza virus RNA polymerase: Identification of the subunit-subunit contact sites on each P protein subunit. J. Gen. Virol. 77, 2149-2157
12. Krug R.M., Ueda, M., and Palese, P. (1975) Temperature-sensitive mutants of influenza WSN virus defective in virus-specific RNA synthesis. J. Virol. 16, 790-796
13. Mahy, B.W.J., Barrett, T., Nichol, S.T., Penn, C.R., and Wolstenholme, A.J. (1981) Analysis of the function of influenza virus genome RNA segments by use of temperature-sensitive mutants of fowl plaque virus in The Replication of Negative Strand Viruses (Bishop, D.H.L. and Compans, R.W., eds.) pp. 379-387, Elsevier, New York
14. Nakagawa, Y., Oda, K., and Nakada, S. (1996) The PB1 subunit alone can catalyze cRNA synthesis, and the PA subunit in addition to the PB1 subunit is required for viral RNA synthesis in replication of the influenza virus genome. J. Virol. 70, 6390-6394
15. 32P]ATP of the large-T proteins of polyoma and SV40 viruses. J. Biol. Chem. 257, 6300-6305
16. Young, J.F., Desselberger, U., Graves, U., Palese, P., Shatzman, A., and Rosenburg, M. (1983) Cloning and expression of influenza virus genes in The Origin of Pandemic Influenza Viruses (Laver, W.G., ed.) pp. 129-138, Elsevier, New York
17. Studier, F.W., Rosenberg, A.H., Dunn, J.J., and Dubendorff, J. W. (1990) Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185, 60-89
18. Kobayashi, M., Tuchiya, K., Nagata, K., and Ishihama, A. (1992) Reconstitution of influenza virus RNA polymerase from three subunits expressed using recombinant baculovirus system. Virus Res. 22, 235-245
19. Honda, A., Mizumoto, K., and Ishihama, A. (1986) RNA polymerase of influenza virus. VII. Dinucleotide-primed initiation of transcription at specific sites on viral RNA. J. Biol. Chem. 261, 5987-5991
20. Kobayashi, M., Toyoda, T., and Ishihama, A. (1996) Influenza virus PB1 protein is the minimal and essential subunit of RNA polymerase. Arch. Virol. 141, 525-533
21. Toyoda, T., Kobayashi, M., Nakada, S., and Ishihama, A. (1996) Molecular dissection of influenza virus RNA polymerase: PB1 subunit alone is able to catalyze RNA synthesis. Virus Genes 12, 155-161
22. Biswas, S.K. and Nayak, D.P. (1994) Mutational analysis of the conserved motifs of influenza A virus polymerase basic protein 1. J. Virol. 68, 1819-1826
23. Toyoda, T., Adyshev, D.M., Kobayashi, M., Iwata, A., and Ishihama, A. (1996) Molecular assembly of influenza virus RNA polymerase: Identification of the subunit-subunit contact sites on each P protein subunit. J. Gen. Virol. 77, 2149-2157
24. Poch, O., Sauvaget, I., Delarue, M., and Tordo, N. (1990) Identification of four conserved motifs among the RNA-dependent polymerase encoding elements. EMBO J. 8, 3867-3874
25. 7GpppNm)-dependent endonuclease. J. Virol. 45, 27-35
26. Mandler, J., Muller, K., and Scholtissek, C. (1991) Mutants and revenants of an avian influenza A virus with temperature-sensitive defects in the nucleoprotein and PB2. Virology 181, 512-519
27. Licheng, S., Summers, D.F., Peng, Q., and Galarza, J.M. (1995) Influenza A virus polymerase subunit PB2 is the endonuclease which cleaves host cell mRNA and functions only as the trimeric enzyme. Virology 208, 38-47
28. Nakagawa, Y., Kimura, N., Toyoda, T., Mizumoto, K., Ishihama, A., Oda, K., and Nakada, S. (1995) The RNA polymerase PB2 subunit is not required for replication of the influenza virus genome but is involved in capped mRNA synthesis. J. Virol. 69, 728-733
29. Shi, L.C., Summers, D.F., Peng, Q.H., and Galarza, J.M. (1995) Influenza A virus RNA polymerase subunit PB2 is the endonuclease which cleaves host cell mRNA and functions only as the trimeric enzyme. Virology 208, 38-47
30. McAllister, W.T. and Raskin, C.A. (1993) The phage RNA polymerase are related to DNA polymerases and reverse transcriptases. Mol. Microbiol. 10, 1-6
31. Richards, O.C., Hanson, J.L., Schultz, S., and Ehrenfeld, E. (1995) Identification of nucleotide binding sites in the poliovirus RNA polymerase. Biochemistry 34, 6288-6295