Genomic species identification of Acinetobacter of clinical isolates by 16S rDNA sequencing

Singapore Medical Journal

Volumn 46, Issue 9, 2005, Pages 461-464

  Misbah, S ^a   Hassan, H ^a   Yusof, M Y ^a   Hanifah, Y A ^a   AbuBakar, Sazaly ^a  

^a UNIVERSITY OF MALAYA   (Malaysia)

Author keywords

16S ribosomal RNA; Acinetobacter; Genomic species identification; Genotyping

Indexed keywords

CYTOSINE; DNA 16S; THYMINE;

ACINETOBACTER BAUMANNII; ARTICLE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; CELL LINEAGE; DNA SEQUENCE; GENE IDENTIFICATION; GENOTYPE; MALAYSIA; NONHUMAN; PHYLOGENY; POLYMERASE CHAIN REACTION; SPECIES IDENTIFICATION;

ACINETOBACTER BAUMANNII; BASE SEQUENCE; DATABASES, GENETIC; DNA, BACTERIAL; DNA, RIBOSOMAL; GENES, BACTERIAL; GENOME; HUMANS; RNA, RIBOSOMAL, 16S;

EID: 24344504125     PISSN: 00375675     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Baumann P. Isolation of Acinetobacter from soil and water. J Bacteriol 1968; 96:39-42.
2. Bergogne-Berezin E, Towner KJ. Acinetobacter spp. as nosocomial pathogens: microbiological, clinical and epidemiological features. Clin Microbiol Rev 1996; 9:148-65.
3. Cisneros JM, Rodriguez-Bano J. Nosocomial bacteremia due to Acinetobacter baumannii: epidemiology, clinical features and treatment. Clin Microbiol Infect 2002; 8:687-93.
4. Carr EL, Kampfer P, Patel BK, et al. Seven novel species of Acinetobacter isolated from activated sludge. Int J Syst Evol Microbiol 2003; 53 (pt 4):953-63.
5. Gerner-Smidt P, Tjernberg I, Ursing J. Reliability of phenotypic tests for identification of Acinetobacter species. J Clin Microbiol 1991; 29:277-82.
6. Gerner-Smidt P, Tjernberg I. Acinetobacter in Denmark: II. Molecular studies of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. APMIS 1993; 101:826-32.
7. Chen CH, Lin LC, Chang YJ, et al. Analysis of prognostic factors in 95 patients with Acinetobacter baumannii bacteremia. Infection 2003; 31:331-5.
8. Kuo LC, Teng LJ, Yu CJ, et al. Dissemination of a clone of unusual phenotype of pandrug-resistant Acinetobacter baumannii at a university hospital in Taiwan. J Clin Microbiol 2004; 42:1759-63.
9. Van de Peer Y, Jansen J, De Rijk P, et al. Database on the structure of small ribosomal subunit RNA. Nucleic Acids Res 1997; 25:111-6.
10. Maidak BL, Olsen GJ, Larsen N, et al. The Ribosomal Database Project (RDP). Nucleic Acids Res 1996; 24:82-5.
11. Liu PY, Wu WL. Use of different PCR-based DNA fingerprinting techniques and pulsed-field gel electrophoresis to investigate the epidemiology of Acinetobacter calcoaceticus-Acinetobacter baumannii complex. Diagn Microbiol Infect Dis 1997; 29:19-28.
12. Spence RP, Towner KJ, Henwood CJ, et al. Population structure and antibiotic resistance of Acinetobacter DNA group 2 and 13TU isolates from hospitals in the UK. J Med Microbiol 2002; 51:1107-12.
13. Janssen P, Maquelin K, Coopman R, et al. Discrimination of Acinetobacter genomic species by AFLP fingerprinting. Int J Syst Bacteriol 1997; 47:1179-87.
14. Chandra R, Kapil A, Sharma P, et al. Identification of Acinetobacter species isolated from clinical specimens by amplified ribosomal DNA restriction analysis. Indian J Med Res 2002; 116:1-4.
15. Snelling AM, Gerner-Smidt P, Hawkey PM, et al. Validation of use of whole-cell repetitive extragenic palindromic sequence-based PCR (REP-PCR) for typing strains belonging to the Acinetobacter calcoaceticus-Acinetobacter baumannii complex and application of the method to the investigation of a hospital outbreak. J Clin Microbiol 1996; 34:1193-202.
16. Misbah S, AbuBakar S, Hassan H, et al. Antibiotic susceptibility and REP-PCR fingerprints of Acinetobacter spp. isolated from a hospital at ten years apart. J Hosp Infect 2004; 58:254-61.
17. Lane DJ. 16S/23S rRNA sequencing. In: Stakebrandt E, Goodfellow M, eds. Nucleic Acid Techniques in Bacterial Systematics. Chichester, New York: Wiley Press, 1991:115-75.
18. Weisburg WG, Barns SM, Pelletier DA, et al. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991; 173:697-703.
19. Drancourt M, Bollet C, Carlioz A, et al. 16S ribosomal DNA sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates. J Clin Microbiol 2000; 38:3623-30.
20. Cilia V, Lafay B, Christen R. Sequence heterogeneities among 16S ribosomal RNA sequences, and their effect on phylogenetic analyses at the species level. Mol Biol Evol 1996; 13:451-61.
21. Yamamoto S, Harayama S. Phylogenetic relationships of Pseudomonas putida strains deduced from the nucleotide sequences of gyrB, rpoD and 16S rRNA genes. Int J Syst Bacteriol 1998; 48(pt3):813-9.