Validation of use of whole-cell repetitive extragenic palindromic sequence-based PCR (REP-PCR) for typing strains belonging to the Acinetobacter calcoaceticus-acinetobacter baumannii complex and application of the method to the investigation of a hospital outbreak

Journal of Clinical Microbiology

Volumn 34, Issue 5, 1996, Pages 1193-1202

  Snelling, Anna M ^a   Gerner Smidt, Peter ^c   Hawkey, Peter M ^a   Heritage, John ^a   Parnell, Peter ^b   Porter, Christine ^b   Bodenham, Andrew R ^b   Inglis, Tim ^a,d  

^a UNIVERSITY OF LEEDS   (United Kingdom)

^b LEEDS GENERAL INFIRMARY   (United Kingdom)

^c STATENS SERUM INSTITUT   (Denmark)

^d NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA;

ACINETOBACTER; ACINETOBACTER CALCOACETICUS; ADULT; ARTICLE; CLINICAL ARTICLE; CLINICAL PROTOCOL; EPIDEMIC; HOSPITAL INFECTION; HUMAN; LABORATORY DIAGNOSIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REPRODUCIBILITY; RIBOTYPING; STRAIN DIFFERENCE;

ACINETOBACTER; ACINETOBACTER CALCOACETICUS; ACINETOBACTER INFECTIONS; ADULT; BACTERIAL TYPING TECHNIQUES; BASE SEQUENCE; CROSS INFECTION; DISEASE OUTBREAKS; DNA PRIMERS; DNA, BACTERIAL; EPIDEMIOLOGY, MOLECULAR; EVALUATION STUDIES; HUMANS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; REPETITIVE SEQUENCES, NUCLEIC ACID; REPRODUCIBILITY OF RESULTS;

EID: 0029968082     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.34.5.1193-1202.1996     Document Type: Article

References (42)

1. Al-Khoja, M. S., and J. H. Darrell. 1979. The skin as the source of Acinctobactcr and Moraxella species occurring in blood cultures J. Clin. Pathol. 32: 497-199.
2. Beck-Sagué, C. M., W. R. Jarvis, J. H. Brook, D. H. Culver, A. Potts, E. Gay, B. W. Shotts, B. Hill, R. L. Anderson, and M. P. Weinstein. 1990 Epidemic bacteremia due to Acinetobacter baumannii in five intensive care units. Am. J. Epidemiol. 132:723-733.
3. Bell, D. A., and D. M. DeMarini. 1991. Excessive cycling converts PCR products to random-length higher molecular weight fragments. Nucleic Acids Res. 19:5079
4. Bergogne-Bérézin, E., and M. L, Joly-Guillou. 1985. An underestimated nosocomial pathogen. Acinetobacter calcoaceticus. J. Antimicrob. Chemother 16:535-538.
5. Bergogne-Bérézin, E., M. L. Joly-Guillou, and J. F. Vieu. 1987. Epidemiology of nosocomial infections due to Acinetobacter calcoaceticus J. Hosp. Infect. 10:105-113.
6. Berthelot, P., F. Grattard, P. Mahul, R. Jospe, B. Pozzetto, A. Ros, O. G. Gaudin, and C. Auboyer. 1993. Ventilator temperature sensors: an unusual source of Pseudomonas cepacia in nosocomial infection. J Hosp. Infect. 25: 33-43.
7. Bouvet, P. J. M., and P. A. D. Grimont. 1986. Taxonomy of the genus Acmetobactei with the recognition of Acmetobacter haumannii sp. nov.,Acinelobacter haemolyticus sp. nov., Acinetobacter johnsonii sp. nov., and Acinetobacter junu sp. nov , and emended descriptions of Acinctobacter calcoaceticus and Acinetobacter lwoffii. Int J. Syst. Bacteriol. 36:228-240.
8. Bouvet, P. J. M., and S. Jeanjean. 1989 Delineation of new proteolytic genomic species in the genus Acinetobactei. Res Microbiol. 140:291-299.
9. Cefai, C., J. Richards, F. K. Gould, and P. McPeake. 1990. An outbreak of Acinetobacter respiratory tract infection resulting from incomplete disinfection of ventilatory equipment. J. Hosp. Infect. 15:177-182.
10. Contant, J., E. Kemeny. C. Oxley, E. Perry, and G. Garber. 1990 Investigation of an outbreak of Acinetobacter calcoacencus var. anitratus infections in an adult intensive caie unit. Am. J. Infect. Control 18:288-291.
11. de Bruijn, F. J. 1992. Use of repetitive (repetitive extragenic palindromic and enterobacterial repetitive intergenic consensus) sequences and the polymerae chain reaction to fingerprint the genomes of Rhizobium melilori isolates and other soil bacteria. Appl Environ Microbiol 58:2180-2187.
12. Dijkshoorn, L., H. M. Aucken, P. Gerner-Sinidt, M. E. Kaufmann, J. Ursing, and T. L. Pitt. 1993. Correlation of typing methods for Acinerobacter isolates from hospital outbreaks. J Clin. Microbiol. 31:702-705.
13. Fagon, J.-Y., J. Chastre, Y. Domart, J.-L. Trouillet, J. Pierre, C. Darne, and C. Gibert. 1989. Nosocomial pneumonia in patients receiving continuous mechanical ventilation. Am. Rev. Respir. Dis. 139:877-884.
14. Gerner-Smidt, P. 1987. Endemic occurrence of Acinetobacter calcoaceticus biovar anitratus in an intensive care unit. J. Hosp. Infect. 10:265-272.
15. Gerner-Smidt, P. 1989. Frequency of plasmids in strains of Acinetobacter calcoaceticus. J. Hosp. Infect. 14:23-28.
16. Gerner-Smidt, P. 1992. Ribotyping of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. J. Clin. Microbiol 30:2680-2685.
17. Gerner-Smidt, P., and I. Tjernberg. 1993 Acinetobacter in Denmark. II. Molecular studies of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. APMIS 101:826-832.
18. Gerner-Smidt, P., I. Tjernberg, and J. Ursing. 1991. Reliability of phenotypic tests for identification of Acinetobacter species. J. Clin. Microbiol. 29: 277-282.
19. Gervich, D. H., and C. S. Grout. 1985. An outbreak of nosocomial Acinetobacter infections from humidifiers Am. J. Infect Control 13:210-215.
20. Getchell-White, S. I., L. G. Donowitz, and D. H. M. Gröschel. 1989. The inanimate environment of an intensive care unit as a potential source of nosocomial bacteria: evidence for long survival of Acinetobacter calcoaceticus. Infect. Control Hosp. Epidemiol. 10:402-407.
21. Goodhart, G. L., E. Abrutyn, R. Watson, R. K. Root, and J. Egert. 1977. Community-acquired Acinetobacter calcoaceticus var. anitratus pneumonia JAMA 238:1516-1518.
22. Gouby, A., M. Carles-Nurit, N. Bouziges, G. Bourg, R. Mesnard, and P. J. M. Bouvet. 1992. Use of pulsed-field gel electrophoresis for investigation of hospital outbreaks of Acinetohacter baumannii. J. Clin. Microbiol 30:1588-1591.
23. Gräser, Y., I. Klare, E. Halle, R. Gantenberg, P. Buchholz, H. D. Jacobi, W. Presber, and G. Schönian. 1993. Epidemiological study of an Acinetobacter baumannii outbreak by using polymerase chain reaction fingerprinting. J. Clin. Microbiol. 31:2417-2420.
24. Hartstein, A. I., A. L. Rashad, J. M. Liebler, L. A. Actis, J. Freeman, J. W. Rourke, T. B. Stibolt, M. E. Tolmasky, G. R. Ellis, and J. H. Crosa. 1988. Multiple intensive care unit outbreak of Acinetobacter calcoacettcus subspecies anitratus respiratory infection and colonization associated with contaminated, reusable ventiiator circuits and resuscitation bags. Am. J. Med 85: 624-631.
25. Hoffmann, S., C. E. Mabeck, and R. Vejsgaard. 1982. Bacteriuria caused by Acinetobacter calcoaceticus biovars in a normal population and in general practice J. Clin. Microbiol. 16:443-451
26. Horrevorts, A., K. Bergman, L. Kollée, I. Breuker, I. Tjernberg, and L. Dijkshoorn. 1995. Clinical and epidemiological investigations of Acinetobacter genomospecies 3 in a neonatal intensive care unit. J. Clin. Microbiol. 33:1567-1572.
27. Hunter, P., and M. Gaston. 1988. Numerical index of discriminatory ability of typing systems: an application of Simpson's index of diversity J. Clin. Microbiol 26:2465-2466
28. Joly-Guillou, M. L., E. Bergogne-Bérézin, and J. F. Vieu. 1990. A study of the relationships between antibiotic resistance phenotypes, phage-typmg and biotyping of 117 clinical isolates of Acinetobacter spp. J. Hosp. Infect. 16: 49-58.
29. Patterson, J. E, J. Vecchio, E. L. Pantelick, P. Farrel, D. Mazon, M. J. Zervos, and W. J. Hierholzer. 1991. Association of contaminated gloves with transmission of Acinetobacter calcoaceticus var. anitratus in an intensive care unit. Am. J. Med. 91:479-483.
30. Reboli, A. C., E. D. Houston, J. S. Monteforte, C. A. Wood, and R. J. Hamill. 1994. Discrimination of epidemic and sporadic isolates of Acinetobacter baumannii by repetitive element PCR-mediated DNA fingerprinting. J. Clin. Microbiol. 32:2635-2640.
31. Schmid, J., E. Voss, and D. R. Soil. 1990. Computer-assisted methods for assessing strain relatedness in Candida albicans by fingerprinting with moderately repetitive sequence Ca3. J. Clin. Microbiol. 28:1236-1243.
32. Seifert, H., R. Baginski, A. Schulze, and G. Pulverer. 1993. Antimicrobial susceptibility of Acinetobacter species Antimicrob. Agents Chemother. 37: 750-753
33. Seifert, H., A. Schulze, R. Baginski, and G. Pulverer. 1994. Plasmid DNA fingerprinting of Acinetobacter species other than Acinetobacter baumannii. J. Clin Microbiol. 32:82-86.
34. Shen, W., and B. Hohn. 1992 DMSO improves PCR amplification of DNA with complex secondary structure. Trends Genet. 8:227.
35. Siegmanigra, Y., S. Baryosef, A. Gorea, and J. Avram. 1993. Nosocomial Acinetobacter meningitis secondary to invasive procedures-report of 25 cases and review Clin. Infect. Dis 17:843-849.
36. Stern, M. J., G. F.-L. Ames, N. H. Smith, E. C. Robinson, and C. F. Higgins. 1984. Repetitive extragenic palindromie sequences: a major component of the bacterial genome. Cell 37:1015-1026
37. Struelens, M. J., E. Cartier, N. Maes, E. Serruys, W. G. V. Quint, and A. van Belkum. 1993. Nosocomial colonization and infection with multiresistant Acinetobacter baumannii: outbreak delineation using DNA macrorestriction analysis and PCR-fingerprinting. J. Hosp. Infect 25:15-32.
38. Tjernberg, I., and J. Ursing. 1989 Clinical strains of Acinetobacter classified by DNA-DNA hybridization. APMIS 97:595-605.
39. Vandenbroucke-Grauls, C. M. J. E., A. J. H. Kerver, J. H. Rommes, R. Jansen, C. den Dekker, and J. Verhoef. 1988. Endemic Acinetobacter anitratus in a surgical intensive care unit: mechanical ventilators as reservoir. Eur. J. Clin. Microbiol. Infect. Dis. 7:485-489.
40. Versalovic, J., T. Koeuth, and J. R. Lupski. 1991. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res 19:6823-6831.
41. Woods, C. R., J. Versalovic, T. Koeuth, and J. R. Lupski. 1992. Analysis of relationships among isolates of Citrobacter diversus by using DNA fingerprints generated by repetitive sequence-based primers in the polymerase chain reaction. J. Clin. Microbíol. 30:2921-2929.
42. Woods, C. R, J. VersaIovic, T. Koeuth, and J. R. Lupski. 1993. Whole-cell repetitive element sequence-based polymerase chain reaction allows rapid assessment of clonal relationships of bacterial isolates. J. Clin. Microbiol. 31: 1927-1931.