Characterization of the PCR inhibitory effect of bile to optimize real-time PCR detection of Helicobacter species

FEMS Immunology and Medical Microbiology

Volumn 44, Issue 2, 2005, Pages 177-182

  Al Soud, Waleed Abu ^a   Ouis, Ibn Sina ^a   Li, Dai Qing ^a   Ljungh, Åsa ^a   Wadström, Torkel ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

Amplification facilitators; Bile; DNA polymerase; Human; Mucin; PCR inhibitors; Porcine

Indexed keywords

BACTERIAL DNA; BILE ACID; BILE SALT; CASEIN; DNA POLYMERASE; FORMAMIDE; PRIMER DNA; PROTEIN; DNA DIRECTED DNA POLYMERASE; MUCIN;

BACTERIUM DETECTION; BILE; CONCENTRATION (PARAMETERS); CONFERENCE PAPER; CONTROLLED STUDY; DILUTION; DNA DETERMINATION; ENZYME STABILITY; GENE AMPLIFICATION; HEATING; HELICOBACTER; HELICOBACTER PYLORI; HUMAN; MALE; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SAMPLE; SPECIES; TEMPERATURE; THERMOSTABILITY; ANIMAL; ARTICLE; CHEMISTRY; CLASSIFICATION; DRUG ANTAGONISM; GENETICS; HEAT; ISOLATION AND PURIFICATION; METHODOLOGY; MOUSE; POLYMERASE CHAIN REACTION; SWINE;

ANIMALS; BILE; BILE ACIDS AND SALTS; DNA-DIRECTED DNA POLYMERASE; ENZYME STABILITY; HEAT; HELICOBACTER; HUMANS; MALE; MICE; MUCINS; POLYMERASE CHAIN REACTION; SWINE;

EID: 24344494455     PISSN: 09288244     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.femsim.2004.12.004     Document Type: Conference Paper

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