Cloning, sequence analysis, and characterization of a novel β-glucosidase-like activity from Pichia etchellsii

Biochemical and Biophysical Research Communications

Volumn 336, Issue 1, 2005, Pages 299-308

  Roy, Pranita ^a   Mishra, Saroj ^a   Chaudhuri, Tapan K ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

Author keywords

Glucosidase like protein; CD spectroscopy; Cell surface associated protein; Fluorescence microscopy; MUG hydrolysis

Indexed keywords

4 NITROPHENYL BETA GLUCOSIDE; ASPARTIC ACID; BETA GLUCOSIDASE; CELL SURFACE PROTEIN; DIPEPTIDE DERIVATIVE; FUNGAL ENZYME; GENTIOBIOSE; GLUCOSIDE; METHYL UMBELLIFERYL BETA DEXTRO GLUCOSIDE; SERINE; UNCLASSIFIED DRUG;

ARTICLE; CIRCULAR DICHROISM; COMPUTER PREDICTION; COMPUTER PROGRAM; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SUBSTRATE; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; GENETIC CODE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; OPEN READING FRAME; PICHIA; PICHIA ETCHELLSII; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN HYDROLYSIS; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; PROTEIN STRUCTURE; SEQUENCE ANALYSIS; STRUCTURAL HOMOLOGY; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; BASE SEQUENCE; BETA-GLUCOSIDASE; CIRCULAR DICHROISM; CLONING, MOLECULAR; DNA, FUNGAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MOLECULAR SEQUENCE DATA; OPEN READING FRAMES; PICHIA; SEQUENCE HOMOLOGY, AMINO ACID;

ESCHERICHIA COLI; PICHIA ETCHELLSII;

EID: 24344480585     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2005.08.067     Document Type: Article

References (33)

1. V.S. Bisaria, and S. Mishra Cellulase biosynthesis and regulation CRC Crit. Rev. Biotechnol. 5 1989 61 103
2. P. Tomme, R.A.J. Warren, and N.R. Gilkes Cellulose hydrolysis by bacteria and fungi Adv. Microbiol. Physiol. 37 1995 1 81
3. A. Esen β-Glucosidases: Biochemistry and Molecular Biology 1993 American Chemical Society Washington, DC
4. B. Brozobohaty, I. Moore, P. Kristofferson, L. Bako, N. Campos, J. Schell, and K. Palme Release of active cytokinin by a β-glucosidase localized to the maize root meristem Science 262 1993 1051 1054
5. N.W. Barton, F.S. Furbish, G.T. Murray, M. Garfield, and R.O. Brady Therapeutic response to intravenous infusions of glucocerebrosidase in patients with Gaucher's disease Proc. Natl. Acad. Sci. USA 87 1990 1913 1916
6. Y. Bhatia, S. Mishra, and V.S. Bisaria Microbial β-glucosidases: cloning, properties and applications CRC Crit. Rev. Biotechnol. 22 2002 375 407
7. P.M. Coutinhu, B. Henrissat, Carbohydrate active enzymes server. Available from: 〈http://afmb.cnrs-mrs.fr/~pedro/CAZY〉 (2004).
8. J.N. Varghese, M. Hrmova, and G.B. Fincher Three-dimensional structure of a barley β-d-glucan exohydrolase, a family 3 glycosyl hydrolase Structure 7 1999 179 190
9. M. Pandey, and S. Mishra Cloning and expression of β-glucosidase gene from the yeast Pichia etchellsii J. Ferm. Bioeng. 80 1995 446 453
10. B. Sethi, M. Jain, M. Chowdhary, Y. Soni, Y. Bhatia, V. Sahai, and S. Mishra Cloning, characterization of Pichia etchellsii β-glucosidase II and effect of media composition and feeding strategy on its production in a bioreactor Biotechnol. Bioprocess Eng. 7 2002 43 51
11. M. Pandey, and S. Mishra Expression and characterization of Pichia etchellsii β-glucosidase in Escherichia coli Gene 190 1997 45 51
12. Y. Bhatia, S. Mishra, and V.S. Bisaria Purification and characterization of recombinant Escherichia coli-expressed Pichia etchellsii β-glucosidase II with high hydrolytic activity on sophorose Appl. Microbiol. Biotechnol. 66 2005 527 535
13. Y. Bhatia, S. Mishra, and V.S. Bisaria Biosynthetic activity of recombinant Escherichia coli expressed Pichia etchellsii β-glucosidase II Appl. Biochem. Biotechnol. 102/103 2002 367 379
14. P. Bacchawat, S. Mishra, Y. Bhatia, and V.S. Bisaria Enzymatic synthesis of oligosaccharides, alkyl and terpene glucosides by recombinant Escherichia coli expressed Pichia etchellsii β-glucosidase II Appl. Biochem. Biotechnol. 118 2004 269 282
15. T. Kannan, D. Loganathan, Y. Bhatia, S. Mishra, and V.S. Bisaria Transglycosylation catalyzed by almonds β-glucosidase and cloned Pichia etchellsii β-glucosidase II using glycosylasparagine mimetics as novel acceptors Biocatal. Biotransform. 22 2004 1 7
16. A. Wallecha, and S. Mishra Purification and characterization of two β-glucosidases from a thermo-tolerant yeast Pichia etchellsii Biochim. Biophys. Acta 1649 2003 74 84
17. T. Maniatis, E.F. Fritsch, and J. Sambrook Molecular Cloning, A Laboratory Manual second ed. 1989 Cold Spring Harbor Laboratory New York
18. J.D. Thompson, D.G. Higgins, and T.J. Gibson CLUSTAL W, improving the sensitivity of progressive multiple sequence alignments through sequence weighting, positions-specific gap penalties and weight matrix choice Nucleic Acids Res. 22 1994 4673 4680
19. L.A. Kelley, R.M. MacCallum, and M.J.E. Sternberg Enhanced genome annotation using structural profiles in the program 3D-PSSM J. Mol. Biol. 299 2000 499 520
20. U.K. Laemmli Cleavage of structural proteins during the assembly of the head bacteriophage T4 Nature 227 1970 680 685
21. S. Gallagher, S.E. Winston, S.A. Fuller, J.G.R. Huriell, in: F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, K. Struhl (Eds.), Current Protocols in Molecular Biology, Analysis of Proteins, Publishing Associates and Wiley Interscience, New York, Unit 10.8, 1993.
22. H. Van Tilbeurgh, F.G. Loontiens, C.K. De Bruyne, and M. Claeyssens Fluorogenic and chromogenic glycosides as substrates and ligands of carbohydrases Methods Enzymol. 160 1988 45 59
23. M.M. Bradford A rapid and sensitive method for the quantitation of the microgram quantities of the protein utilizing the principle of protein dye binding Anal. Biochem. 72 1976 248 254
24. T.K. Chaudhuri, K. Horii, T. Yoda, M. Arai, S. Nagata, T.P. Terada, H. Uchiyama, T. Ikura, K. Tsumoto, H. Kataoka, M. Matsushima, K. Kuwajima, and I. Kumagai Effect of extra N-terminal methionine residue on stability and folding of recombinant α-lactalbumin expressed in Escherichia coli J. Mol. Biol. 285 1999 1179 1194
25. J.T. Yang, C.S. Wu, and H.M. Martinez Calculation of protein conformation from circular dichroism Methods Enzymol. 130 1986 208 269
26. D. Voet, and J.G. Voet Biochemistry second ed. 1995 Wiley New York
27. D. McDevitt, P. Francois, P. Vandaux, and T.J. Foster Molecular characterization of the clumping factor (fibrinogen receptor) of Staphylococcus aureus Mol. Microbiol. 11 1994 237 248
28. D. NiEidhin, S. Perkins, P. Francois, P. Vandaux, M. Hook, and T.J. Foster Clumping factor B (ClfB), a new surface-located fibrinogen-binding adhesin of Staphylococcus aureus Mol. Microbiol 29 1998 245 257
29. E. Josefsson, K.W. McCrea, D.N. Eidhin, D.O. Connell, J. Cox, M. Hook, and T.J. Foster Three new members of the serine-aspartate repeat protein multigene family of Staphylococcus aureus Microbiology 144 1998 3387 3395
30. K.W. McCrea, O. Hartford, S. Davis, D.N. Eidhin, G. Lina, P. Speziale, T.J. Foster, and M. Hook The serine-asparatate repeat (Sdr) protein family in Staphylococcus epidermidis Microbiology 146 2000 1535 1546
31. A. Andreeva, D. Howorth, S.E. Brenner, T.J.P. Hubbard, C. Chothia, and A.G. Murzin SCOP database in 2004: refinements integrate structure and sequence family data Nucleic Acids Res. 32 2004 D226 D229
32. A. Wallecha, Purification and characterization of two β-glucosidases from a thermo-tolerant yeast Pichia etchellsii, Ph.D. thesis, Indian Institute of Technology Delhi, 2002.
33. G.D. Fasman Circular Dichroism and the Conformational Analysis of Biomolecules 1996 Plenum press New York