Purification and characterization of recombinant Escherichia coli-expressed Pichia etchellsii β-glucosidase II with high hydrolytic activity on sophorose

Applied Microbiology and Biotechnology

Volumn 66, Issue 5, 2005, Pages 527-535

  Bhatia, Yukti ^a   Mishra, Saroj ^a   Bisaria, Virendra S ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BIOCATALYSTS; BIOSYNTHESIS; DATA ACQUISITION; DIMERS; ENZYME KINETICS; GENES; YEAST;

DIETHYLPYROCARBONATE MODIFICATION; OLIGOSACCHARIDES; PICHIA ETCHELLSII; SOPHOROSE;

ESCHERICHIA COLI;

4 NITROPHENYL BETA GLUCOSIDE; AMYGDALIN; BETA GLUCOSIDASE; DIETHYL PYROCARBONATE; DIMER; DISACCHARIDE; FUNGAL ENZYME; GLUCOSIDE; GLYCOSYLTRANSFERASE; OLIGOSACCHARIDE; RECOMBINANT ENZYME; SALICIN; SOPHOROSE; UNCLASSIFIED DRUG;

BIOTECHNOLOGY; ENZYME ACTIVITY;

AMINO ACID COMPOSITION; AMINO ACID SEQUENCE; ARTICLE; BIOCATALYST; CARBOHYDRATE SYNTHESIS; CATALYSIS; CHEMICAL MODIFICATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENETIC CODE; HYDROLYSIS; MOLECULAR WEIGHT; NONHUMAN; PICHIA; PICHIA ETCHELLSII; PROTEIN FAMILY;

BETA-GLUCOSIDASE; CLONING, MOLECULAR; ESCHERICHIA COLI; GLUCANS; HYDROLYSIS; KINETICS; PICHIA; RECOMBINANT PROTEINS; TEMPERATURE;

ESCHERICHIA COLI; PICHIA; PICHIA ETCHELLSII;

EID: 12544255667     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-004-1754-8     Document Type: Article

References (40)

1. Ajisaka K, Nishida H, Fujimoto H (1987) The synthesis of oligosaccharides by the reversed hydrolysis reaction of β-glucosidase at high temperature. Biotechnol Lett 9:243-248
2. Bacchawat P, Mishra S, Bhatia Y, Bisaria VS (2004) Enzymatic synthesis of oligosaccharides, alkyl- and terpene glucosides by recombinant Escherichia coli expressed Pichia etchellsii β-glucosidase II. Appl Biochem Biotechnol 118:269-282
3. Bhatia Y, Mishra S, Bisaria VS (2002a) Microbial β-glucosidases: cloning, properties and applications. Crit Rev Biotechnol 22:375-407
4. Bhatia Y, Mishra S, Bisaria VS (2002b) Biosynthetic activity of recombinant Escherichia coli expressed Pichia etchellsii β-glucosidase II. Appl Biochem Biotechnol 102/103:367-379
5. Burnstein Y, Walsh KA, Neurath H (1974) Evidence of an essential histidine residue in thermolysin. Biochemistry 13:205-210
6. Chen Q-X, Zhang Z, Zhou X-W, Zhuang Z-L (2000) Kinetics of inhibition of β-glucosidase from Ampullarium crossean by bromoacetic acid. Int J Biochem Cell Biol 32:717-723
7. Chou PY (1989) Prediction of protein structural classes from amino acid compositions. In: Fasman GD (ed) Prediction of protein structure and the principles of protein conformation. Plenum, New York, pp 549-586
8. Clarke AJ (1990) Chemical modification of a β-glucosidase from Schizophyllum commune: evidence for essential carboxyl groups. Biochim Biophys Acta 1040:145-152
9. Coutinho PM, Henrisaat B (2004) Carbohydrate active enzymes server. http://afmb.cnrs-mrs.fr/~pedro/CAZY
10. Fersht A (1985) Enzyme structure and mechanism, 2nd edn. Freeman, New York
11. Gallagher S, Winston SE, Fuller SA, Huriell JGR (1993) Unit 10.8. Analysis of proteins. In: Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds) Current protocols in molecular biology. Wiley Interscience, New York
12. Geurtsen R, Cote F, Hahn MG, Boons GJ (1999) Chemoselective glycosylation strategy for the convergent assembly of phytoalexin-elicitor active oligosaccharides and their photoreactive derivatives. J Org Chem 64:7828-7835
13. Gunata Z, Vallier MJ, Sapis JC, Baumes R, Bayonove C (1994) Enzymic synthesis of monoterpenyl β-D-glucosides by various β-glucosidases. Enzyme Microb Technol 16:1055-1058
14. Hiromi K, Hamauzu Z, Takahashi K, Ono S (1966) Kinetic studies on glucoamylase II. Competition between two types of substrates having α1→4 and α1→6 glucosidic linkages. J Biochem 59:411-418
15. Iwashita K, Nagahara T, Kimura H, Takano M, Shimoi H, Ito K (1999) The bglA gene of Aspergillus kawachii encodes both extracellular and cell wall bound β-glucosidases. Appl Environ Microbiol 65:5546-5553
16. Kannan T, Loganathan D, Bhatia Y, Mishra S, Bisaria VS (2004) Transglycosylation catalyzed by almonds β-glucosidase and cloned Pichia etchellsii β-glucosidase II using glycosylasparagine mimetics as novel acceptors. Biocatal Biotransform 22:1-7
17. Kempton JB, Withers SG (1992) Mechanism of Agrobacterium β-glucosidase: kinetic studies. Biochemistry 31:9961-9969
18. Kumble KD, Kumble S, Jaffar MB (1992) Inactivation of β-glucosidase from Arthrobotrys conoides by diethylpyrocarbonate: evidence of histidine at the active site. Indian J Exp Biol 30:99-102
19. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
20. Leah R, Kiegel J, Suendson IB, Mundy J (1995) Biochemical and molecular characterization of a barley seed β-glucosidase. J Biol Chem 22:15789-15796
21. Levy MM, Leber PD, Ryan EM (1963) Inactivation of myosin by 2,4-dinitrophenol and protection by adenosine triphosphate and other phosphate compounds. J Biol Chem 238:3654-3659
22. Machida M, Ohtsuki I, Fukui S, Yamashita I (1988) Nucleotide sequences of Saccharomycopsis fibuligera gene for extracellular β-glucosidases as expressed in Saccharomyces cerevisiae. Appl Environ Microbiol 54:3147-3155
23. Marana SR, Jacobs-Lorena M, Terra WR, Ferreira C (2001) Amino acid residues involved in substrate binding and catalysis in an insect digestive β-glycosidase. Biochim Biophys Acta 1545:41-52
24. Mata IH, Castillon MP, Dominguez JM, Macarron R, Acebal C (1993) Chemical modification of β-glucosidase from Trichoderma reesei QM9414. J Biochem 114:754-759
25. Muhlrad A, Hegyl G, Toth G (1967) Effect of diethylpyrocarbonate on proteins. I. Reaction of diethylpyrocarbonate with amino acids. Acta Biochim Biophys Acad Sci Hung 2:19-29
26. Namchuk MN, Withers SG (1995) Mechanism of Agrobacterium β-glucosidase: kinetic analysis of the role of non covalent enzyme/substrate interactions. Biochemistry 34:16194-16202
27. Nossal NG, Heppel LA (1966) The release of enzymes by osmotic shock from Escherichia coli in exponential phase. J Biol Chem 241:3055-3062
28. Ovadi J, Libor S, Elodi P (1967) Spectrophotometric determination of histidine in proteins with diethyl pyrocarbonate. Acta Biochim Biophys Acad Sci Hung 2:455-458
29. Pandey M, Mishra S (1995) Cloning and expression of β-glucosidase gene from the yeast Pichia etchellsii. J Ferment Bioeng 80:446-453
30. Pandey M, Mishra S (1997) Expression and characterization of Pichia etchellsii β-glucosidase in Escherichia coli. Gene 190 45-51
31. Segal IH (1975) Enzyme kinetics. Wiley, New York
32. Sethi B, Jain M, Chowdhary M, Soni Y, Bhatia Y, Sahai V, Mishra S (2002) Cloning, characterization of Pichia etchellsii β-glucosidase II and effect of media composition and feeding strategy on its production in a bioreactor. Biotechnol Bioprocess Eng 7:43-51
33. Tomme P, Chauvaux S, Beguin P, Millet J, Aubert J-P, Claeyssens M (1991) Identification of a histidyl residue in the active center of endoglucanase D from Clostridium thermocellum. J Biol Chem 266:10313-10318
34. Umezurike GM (1991) Octameric structure of β-glucosidase from Botryodiplodia theobromae Pat. Biochem J 275:721-725
35. Varghese JN, Hrmova M, Fincher GB (1999) Three-dimensional structure of a barley β-D-glucan exohydrolase, a family 3 glycosyl hydrolase. Struct 7:179-190
36. Vic G, Thomas D, Crout DHG (1997) Solvent effect on enzyme catalyzed synthesis of β-D-glucosides using the reverse hydrolysis method: application to the preparative scale synthesis of 2-hydroxybenzyl and octyl-β-D-glucopyranosides. Enzyme Microb Technol 20:597-603
37. Voet D, Voet JG (1995) Biochemistry, 2nd edn. Wiley, New York
38. Wallecha A, Mishra S (2003) Purification and characterization of two β-glucosidases from a thermo-tolerant yeast Pichia etchellsii. Biochim Biophys Acta 1649:74-84
39. Wang PG, Bertozzi CR (2001) Glycochemistry: principles, synthesis, and applications. Dekker, New York
40. 14 β-glucosidase with cellodextrinase and cyanoglycosidase activities. J Bacteriol 177:5884-5890