Subunit assembly modulates the activities of the Type III restriction-modification enzyme PstII in vitro

Nucleic Acids Research

Volumn 33, Issue 15, 2005, Pages 4788-4796

  Sears, Alice ^a   Szczelkun, Mark D ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; METHYLTRANSFERASE; PSTII ENDONUCLEASE; RESTRICTION ENDONUCLEASE; RESTRICTION ENDONUCLEASE TYPE III; S ADENOSYLMETHIONINE; TRITON X 100; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; DNA METHYLTRANSFERASE; OCTOXINOL; PROTEIN SUBUNIT; TYPE III SITE SPECIFIC DEOXYRIBONUCLEASE;

ARTICLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DNA DEGRADATION; ENZYME ACTIVITY; ENZYME DEGRADATION; ENZYME MECHANISM; ENZYME METABOLISM; ENZYME MODIFICATION; ENZYME REGULATION; ENZYME SUBSTRATE COMPLEX; ENZYME SUBUNIT; IN VIVO STUDY; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; PROTEIN METHYLATION; RESTRICTION MAPPING; STOICHIOMETRY; DNA METHYLATION; ENZYME SPECIFICITY; KINETICS; METABOLISM;

BACTERIAL PROTEINS; DEOXYRIBONUCLEASES, TYPE III SITE-SPECIFIC; DNA; DNA METHYLATION; DNA MODIFICATION METHYLASES; KINETICS; OCTOXYNOL; PROTEIN SUBUNITS; SUBSTRATE SPECIFICITY;

EID: 24144441025     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gki788     Document Type: Article

References (26)

1. Sears,A., Peakman,L.J., Wilson,G.G. and Szczelkun,M.D. (2005) Characterisation of the Type III restriction endonuclease PstII from Providencia stuartii. Nucleic Acids Res., 33, 4775-4787.
2. Prakash-Cheng,A. and Ryu,J. (1993) Delayed expression of in vivo restriction activity following conjugal transfer of Escherichia coli hsdK (restriction-modification) genes. J. Bacteriol., 175 4905-4906.
3. Prakash-Cheng,A., Chung,S.S. and Ryu,J. (1993) The expression and regulation of hsdK genes after conjugative transfer. Mol. Gen. Genet., 241, 491-496.
4. Murray,N.E. (2000) Type I restriction systems: Sophisticated molecular machines (a legacy of Bertani and Weigle). Microbiol. Mol. Biol. Rev. 64, 412-434.
5. Makovets,S., Doronina,V.A. and Murray,N.E. (1999) Regulation of endonuclease activity by proteolysis prevents breakage of unmodified bacterial chromosomes by type I restriction enzymes. Proc. Natl Acad. Sci. USA, 96, 9757-9762.
6. Makovets,S., Powell,L.M., Titheradge,A.J., Blakely,G.W. and Murray,N.E. (2004) Is modification sufficient to protect a bacterial chromosome from a resident restriction endonuclease? Mol. Microbiol., 51, 135-147.
7. Bourniquel,A.A. and Bickle,T.A. (2002) Complex restriction enzymes: NTP-driven molecular motors. Biochimie, 84, 1047-1059.
8. Dryden,D.T.F., Cooper,L.P., Thorpe,P.H. and Byron,O. (1997) The in vitro assembly of the EcoKI type I DNA restriction/modification enzyme and its in vivo implications. Biochemistry, 36, 1065-1076.
9. Loenen,W.A., Daniel,A.S., Braymer,H.D. and Murray,N.E. (1987) Organization and sequence of the hsd genes of Escherichia coli K-12. J. Mol. Biol., 198, 159-170.
10. Kulik,E.M. and Bickle,T.A. (1996) Regulation of the activity of the type IC EcoR124I restriction enzyme. J. Mol. Biol., 264, 891-906.
11. Redaschi,N. and Bickle,T.A. (1996) Posttranscriptional regulation of EcoP1I and EcoP15I restriction activity. J. Mol. Biol., 257, 790-803.
12. Keatch,S.A., Su,T.J. and Dryden,D.T. (2004) Alleviation of restriction by DNA condensation and non-specific DNA binding ligands. Nucleic Acids Res., 32, 5841-5850.
13. Efimova,E.P.,Delver,E.P. and Belogurov,A.A. (1988) Alleviation of type I restriction in adenine methylase (dam) mutants of Escherichia coli. Mol. Gen. Genet., 214, 313-316.
14. Janscak,P., Abadjieva,A. and Firman,K. (1996) The type I restriction endonuclease R.EcoR124I: Over-production and biochemical properties. J. Mol. Biol., 257, 977-991.
15. Janscak,P., Dryden,D.T.F. and Firman,K. (1998) Analysis of the subunit assembly of the typeIC restriction modification enzyme EcoR124I. Nucleic Acids Res., 26, 4439-4445.
16. De Backer,O. and Colson,C. (1991) Transfer of the genes for the StyLTI restriction-modification system of Salmonella typhimurium to strains lacking modification ability results in death of the recipient cells and degradation of their DNA. J. Bacteriol., 173, 1328-1330.
17. Janscak,P., Sandmeier,U., Szczelkun,M.D. and Bickle,T.A. (2001) Subunit assembly and mode of DNA cleavage of the type III restriction endonucleases EcoP1I and EcoP15I. J. Mol. Biol., 306, 417-431.
18. Peakman,L.J., Antognozzi,M., Bickle,T.A., Janscak,P. and Szczelkun,M.D. (2003) S-adenosyl methionine prevents promiscuous DNA cleavage by the EcoP1I type III restriction enzyme. J. Mol. Biol., 333, 321-335.
19. Szczelkun,M.D., Dillingham,M.S., Janscak,P., Firman,K. and Halford,S.E. (1996) Repercussions of DNA tracking by the type IC restriction endonuclease EcoR124I on linear, circular and catenated substrates. EMBO J., 15, 6335-6347.
20. Hadi,S.M., Bachi,B., Iida,S. and Bickle,T.A. (1983) DNA restriction-modification enzymes of phage P1 and plasmid p15B. Subunit functions and structural homologies. J. Mol. Biol., 165, 19-34.
21. Rao,D.N., Page,M.G. and Bickle,T.A. (1989) Cloning, over-expression and the catalytic properties of the EcoP15 modification methylase from Escherichia coli. J. Mol. Biol., 209, 599-606.
22. Bist,P., Sistla,S., Krishnamurthy,V., Acharya,A., Chandrakala,B. and Rao,D.N. (2001) S-adenosyl-L-methionine is required for DNA cleavage by type III restriction enzymes. J. Mol. Biol., 310 93-109.
23. Conlan,L.H., Jose,T.J., Thornton,K.C. and Dupureur,C.M. (1999) Modulating restriction endonuclease activities and specificities using neutral detergents. Biotechniques, 27, 955-960.
24. Jobbagy,Z., Izsvak,Z. and Duda,E. (1992) Positive co-operative interaction between the subunits of CegI restriction endonuclease. Biochem. J., 286, 85-88.
25. Halford,S.E., Welsh,A.J. and Szczelkun,M.D. (2004) Enzyme-mediated DNA looping. Annu. Rev. Biophys. Biomol. Struct., 33, 1-24.
26. Szczelkun,M.D. and Halford,S.E. (1996) Recombination by resolvase to analyse DNA communications by the SfiI restriction endonuclease. EMBO J., 15, 1460-1469.