The type I restriction endonuclease R.EcoR124I: Over-production and biochemical properties

Journal of Molecular Biology

Volumn 257, Issue 5, 1996, Pages 977-991

  Janscak, Pavel ^a   Abadjieva, Agnes ^a   Firman, Keith ^a  

^a UNIVERSITY OF PORTSMOUTH   (United Kingdom)

Author keywords

ATPase; Cofactors; DNA cleavage; Restriction modification; Supercoiled DNA

Indexed keywords

ADENOSINE TRIPHOSPHATE; MAGNESIUM ION; RESTRICTION ENDONUCLEASE;

ARTICLE; BIOCHEMISTRY; CONTROLLED STUDY; DNA CLEAVAGE; DNA METHYLATION; DNA SUPERCOILING; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME STRUCTURE; ENZYME SYNTHESIS; NONHUMAN; PLASMID; PRIORITY JOURNAL; STOICHIOMETRY;

EID: 0029968248     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1996.0217     Document Type: Article

References (51)

1. Bickle, T. A. (1987). DNA restriction and modification systems. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology (Ingraham, J. L., Low, K. B., Magasanik, B., Neidhardt, F. C., Schaechter, M. & Umbarger, H. E., eds), pp. 692-696, American Society for Microbiology Washington DC.
2. Bickle, T. A. & Krüger, D. H. (1993). Biology of DNA restriction. Microbiol. Rev. 57, 434-450.
3. Bolivar, F., Rodriguez, R. L., Greene, P. J., Betlach, M. F. & Heyneker, H. (1977). Construction and characterization of new cloning vehicles. 1. Ampicillin-resistant derivatives of the plasmid pMB9. Gene, 2, 95-113.
4. Burckhardt, J., Weisemann, J. & Yuan, R. (1981). Characterisation of the DNA methylase activity of the restriction enzyme from Escherichia coli K. J. Biol. Chem. 256, 4024-4032.
5. 2+-stimulated ATPase activity. Anal. Biochem. 157, 375-380.
6. Chang, A. C. Y. & Cohen, S. N. (1978). Construction and characterisation of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134, 1141-1156.
7. Cowan, G. M., Gann, A. A. F. & Murray, N. E. (1989). Conservation of complex DNA recognition domains between families of restriction enzymes. Cell, 56, 103-109.
8. Dryden, D. T. F., Cooper, L. P. & Murray, N. E. (1993). Purification and characterisation of the methyltransferase from the type I restriction and modification system of Escherichia coli K12. J. Biol. Chem. 268, 13228-13236.
9. Endlich, B. & Linn, S. (1985). The DNA restriction endonuclease of Escherichia coli B. II. Further studies of the structure of DNA intermediates and products. J. Biol. Chem. 260, 5729-5738.
10. Eskin, B. & Linn, S. (1972). The deoxyribonucleic acid modification and restriction enzymes of Escherichia coli B. II. Purification subunit structure and catalytic properties of the restriction endonuclease. J. Biol. Chem. 247, 6183-6191.
11. Firman, K., Price, C. & Glover, S. W. (1985). The EcoR124 and EcoR124/3 restriction and modification systems: cloning the genes. Plasmid, 14, 224-234.
12. Fuller-Pace, F. V. & Murray, N. E. (1986). Two DNA recognition domains of the specificity polypeptides of a family of the type I restriction enzymes. Proc. Natl Acad. Sci. USA, 83, 9368-9372.
13. Gann, A. F. F., Campbell, A. J. B., Collins, J. F., Coulson, A. F. W. & Murray, N. E. (1987). Reassortment of DNA recognition domains and the evolution of new specificities. Mol. Microbiol. 1, 13-22.
14. Glover, S. W. (1970). Functional analysis of host-specificity mutants in Escherichia coli. Genet. Res. (Cambs), 15, 237-250.
15. Gubler, M., Braguglia, D., Meyer, J., Piekarowicz, A. & Bickle, T. A. (1992). Recombination of constant and variable modules alters DNA sequence recognition by type IC restriction-modification enzymes. EMBO J. 11, 233-240.
16. Hadi, S. M., Bickle, T. A. & Yuan, R. (1975). The role of S-adenosyl methionine in the cleavage of deoxyribonucleic acid by the restriction endonuclease from Escherichia coli K. J. Biol. Chem. 250, 4159-4164.
17. + R-factor of a new compatibility class. J. Gen. Microbiol. 71, 403-405.
18. Horiuchi, K. & Zinder, N. D. (1972). Cleavage of bacteriophage f1 DNA by the restriction enzyme of Escherichia coli B. Proc. Natl Acad. Sci. USA, 69, 3220-3224.
19. Hubacek, J. & Glover, S. W. (1970). Complementation analysis of temperature-sensitive host specificity mutations in Escherichia coli. J. Mol. Biol. 50, 111-127.
20. Jacob, F. & Wollman, E. L. (1954). Etude genetique d'un bacteriophage tempere d'Escherichia coli: Le systeme genetique du bacteriophage lambda. Annu. Inst. Pasteur, 87, 653-673.
21. Jobling, M. G. & Holmes, R. K. (1990). Construction of vectors with the p15a replicon, kanamycin resistance, inducible lacZα and pUC18 or pUC19 multiple cloning sites. Nucl. Acids Res. 18, 5315-5316.
22. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.
23. Loenen, W. A. M., Daniel, A. S., Braymer, H. D. & Murray, N. E. (1987). Organization and sequence of the hsd genes of Escherichia coli K-12. J. Mol. Biol. 198, 159-170.
24. Maniatis, T., Fritsch, E. F. & Sambrook, J. (1982). Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
25. Mead, D. A., Szczesna-Skorupa, E. & Kemper, B. (1986). Single-stranded DNA 'blue' T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering. Protein Eng. 1, 67-74.
26. Meselson, M. & Yuan, R. (1968). DNA restriction enzyme from Escherichia coli. Nature, 217, 1110-1114.
27. Nagaraja, V., Shepherd, J. C. W. & Bickle, T. A. (1985a). A hybrid recognition sequence in a recombinant restriction enzyme and the evolution of DNA sequence specificity. Nature, 316, 371-372.
28. Nagaraja, V., Shepherd, J. C. W., Pripfl, T. & Bickle, T. A. (1985b). Two type I restriction enzymes from Salmonella species. Purification and DNA recognition sequences. J. Mol. Biol. 182, 579-587.
29. Patel, J., Taylor, I., Dutta, C. F., Kneale, G. G. & Firman, K. (1992). High-level expression of the cloned genes encoding the subunits of and the intact DNA methyltransferase, M.EcoR124. Gene, 112, 21-27.
30. Piekarowicz, A., Skrzypek, E. & Goguen, J. D. (1985). The EcoDXXI restriction and modification system of Escherichia coli ET7. In Gene Manipulation and Expression (Glass, R. E. & Spizek, J., eds), pp. 79-94, Croom Helm, London.
31. Powell, L. M. & Murray, N. E. (1995). S-adenosyl methionine alters the DNA contacts of the EcoKI methyltransferase. Nucl. Acids Res. 23, 967-974.
32. Powell, L. M., Dryden, D. T. F., Willock, D. F., Pain, R. H. & Murray, N. E. (1993). DNA recognition by the EcoK methyltransferase: the influence of DNA methylation and the cofactor S-adenosyl methionine. J. Mol. Biol. 234, 60-71.
33. K (restriction-modification) genes. J. Bacteriol. 175, 4905-4906.
34. K genes after conjugative transfer. Mol. Gen. Genet. 241, 491-496.
35. Price, C., Pripfl, T. & Bickle, T. A. (1987). EcoR124 and EcoR124/3: the first members of a new family of type I restriction and modification systems. Eur. J. Biochem. 167, 111-115.
36. Singleton, C. K. & Wells, R. D. (1982). The facile generation of covalently closed, circular dnas with defined negative superhelical densities. Anal. Biochem. 122, 253-257.
37. Skrzypek, E. & Piekarowicz, A. (1989). The EcoDXXI restriction and modification system: cloning the genes and homology to type I restriction and modification systems. Plasmid, 21, 195-204.
38. Studier, F. W. & Bandyopadhyay, P. K. (1988). Model for how type I restriction enzymes select cleavage sites in DNA. Proc. Natl Acad. Sci. USA, 85, 4677-4681.
39. Suri, B. & Bickle, T. A. (1985). EcoA: the first member of a new family of type I resriction modification systems. Gene organization and enzymatic activities. J. Mol. Biol. 186, 77-85.
40. -: enzyme structure and DNA recognition sequence. Cold Spring Harbor Symp. Quant. Biol. 43, 1217-1221.
41. Taylor, I., Patel, J., Firman, K. & Kneale, G. G. (1992). Purification and biochemical characterisation of the EcoR124 modification methylase. Nucl. Acids Res. 20, 179-186.
42. Taylor, I., Watts, D. & Kneale, G. (1993). Substrate recognition and selectivity in the type IC DNA modification methylase M.EcoR124I. Nucl. Acids Res. 21, 4929-4935.
43. Taylor, I. A., Davis, K. G., Watts, D. & Kneale, G. G. (1994). DNA binding induces a major structural transition in a type I methyltransferase. EMBO J. 13, 5772-5778.
44. Tyndall, C., Meister, J. & Bickle, T. A. (1994). The Escherichia coli prr region encodes a functional type IC DNA restriction system closely integrated with an anticodon nuclease gene. J. Mol. Biol. 237, 266-274.
45. Weiserova, M., Janscak, P., Benada, O., Hubacek, J., Zinkevich, V., Glover, S. W. & Firman, K. (1993). Cloning, production and characterisation of wild type and mutant forms of the R.EcoK endonucleases. Nucl. Acids Res. 21, 373-379.
46. Willcock, D. F., Dryden, D. T. F. & Murray, N. E. (1994). A mutational analysis of the two motifs common to adenine methyltransferases. EMBO J. 13, 3902-3908.
47. Wilson, G. G. & Murray, N. E. (1991). Restriction and modification systems. Annu. Rev. Genet. 25, 585-627.
48. Yanisch-Perron, C., Vieira, J. & Messing, J. (1985). Improved M13 phage cloning vectors and host strains: nucleotide sequence of the M13mp18 and pUC19 vectors. Gene, 33, 103-119.
49. Yuan, R., Heywood, J. & Meselson, M. (1972). ATP hydrolysis by restriction endonuclease from E. coli K. Nature New Biol. 240, 42-43.
50. Yuan, R., Bickle, T. A., Ebbers, W. & Brack, C. (1975). Multiple steps in DNA recognition by restriction endonuclease from E. coli K. Nature, 256, 556-560.
51. Yuan, R., Hamilton, D. L. & Burckhardt, J. (1980). DNA translocation by the restriction enzyme from E. coli K. Cell, 20, 237-244.