Normal physiological conditions maintain the biological characteristics of porcine aortic heart valves: An ex vivo organ culture study

Annals of Biomedical Engineering

Volumn 33, Issue 9, 2005, Pages 1158-1166

  Konduri, Suchitra ^a,c   Xing, Yun ^a,c   Warnock, James N ^b,c   He, Zhaoming ^c   Yoganathan, Ajit P ^c,d  

^a Georgia Institute of Technology   (United States)

^b GEORGIA INSTITUTE OF TECHNOLOGY   (United States)

^c GEORGIA INSTITUTE OF TECHNOLOGY   (United States)

^d Emory University   (United States)

Author keywords

Aortic valve leaflets; Biological properties; Organ culture system; Phenotype

Indexed keywords

AORTIC VALVE LEAFLETS; BIOLOGICAL PROPERTIES; ORGAN CULTURE SYSTEM; PHENOTYPE;

BIOLOGICAL ORGANS; BIOMEDICAL ENGINEERING; CELL CULTURE; COLLAGEN; PHYSIOLOGY; TISSUE;

HEART VALVE PROSTHESES;

COLLAGEN; ELASTIN;

ANIMAL; AORTA VALVE; APOPTOSIS; ARTICLE; BIOSYNTHESIS; CELL PROLIFERATION; ENDOTHELIUM CELL; HEART OUTPUT; MECHANICAL STRESS; MECHANOTRANSDUCTION; METABOLISM; ORGAN CULTURE TECHNIQUE; PHYSIOLOGY; SWINE; ULTRASTRUCTURE;

ANIMALS; AORTIC VALVE; APOPTOSIS; CARDIAC OUTPUT; CELL PROLIFERATION; COLLAGEN; ELASTIN; ENDOTHELIAL CELLS; MECHANOTRANSDUCTION, CELLULAR; ORGAN CULTURE TECHNIQUES; STRESS, MECHANICAL; SWINE;

EID: 24144439141     PISSN: 00906964     EISSN: 15739686     Source Type: Journal    
DOI: 10.1007/s10439-005-5506-4     Document Type: Conference Paper

References (23)

1. Butcher, J. T., A. M. Penrod, A. J. Garcia, and R. M. Nerem. Unique morphology and focal adhesion development of valvular endothelial cells in static and fluid flow environments. Aerterioscler. Thromb. Vasc. Biol. 24:1429-1434, 2004.
2. Butcher, J. T., and R. M. Nerem. Porcine aortic valve interstitial cells in three-dimensional culture: Comparison of phenotype with aortic smooth muscle cells. J. Heart Valve Dis. 13:478-486, 2004.
3. Butler, D. L., S. A. Goldstein, and F. Guilak. Functional tissue engineering: The role of biomechanics. J. Biomech. Eng. 122:570-575, 2000.
4. Cimini, M., K. A. Rogers, and D. R. Boughner. Aortic valve interstitial cells: An evolution of cell viability and cell phenotype over time. J. Heart Valve Dis. 11:881-887, 2002.
5. Davies, P. F., K. A Barbee, M. V. Volin, A. Robotewskyj, J. Chen, L. Joseph, M. L. Griem, M. N. Wernick, E. Jacobs, D. C. Polacek, N. de Paola, and A. I. Barakat. Spatial relationships in early signaling events of flow-mediated endothelial mechanotransduction. Annu. Rev. Physiol. 59:527-549, 1997.
6. Deck, J. D. Endothelial cell orientation on aortic valve leaflets. Cardiovasc. Res. 20:760-767, 1986.
7. Dumont, K., J. Yperman, E. Verbeken, P. Segers, B. Meuris, S. Vandenberghe, W. Flameng, and P. R. Verdonck. Design of a new pulsatile bioreactor for tissue engineered aortic heart valve formation. Artif. Organs. 26:710-714, 2002.
8. Hildebrand, D. K., Z. J. Wu, J. E. Mayer Jr., and M. S. Sacks. Design and hydrodynamic evaluation of a novel pulsatile bioreactor for biologically active heart valves. Ann. Biomed. Eng. 32:1039-1049, 2004.
9. Hoerstrup, S. P., R. Sodian, J. S. Sperling, J. P. Vacanti, and J. E. Mayer Jr. New pulsatile bioreactor for in vitro formation of tissue engineered heart valves. Tissue Eng. 6:75-79, 2000.
10. Layland, K. S., I. Reimann, F. Opitz, K. J. Halbhuber, and U. A. Stock. Comparitive study of cellular and extracellular matrix composition of native and tissue engineered heart valves. Matrix Biol. 23:113-125, 2004.
11. Leask, R. L., N. Jain, and J. Butany. Endothelium and valvular diseases of the heart. Microsc. Res. Technol. 60:129-137, 2003.
12. Quick, D. W., K. S. Kunzelman, J. M. Kneebone, and R. P. Cochran. Collagen synthesis is upregulated in mitral valves subjected to altered stress. ASAIO J. 43:181-186, 1997.
13. Schneider, P. J., and J. D. Deck. Tissue and cell renewal in the natural aortic valve of rats: An autoradiography study. Cardiovasc. Res. 15:181-189, 1981.
14. Shappell, S. D., Valvular heart diseases. http://www.heartcenteronline. com/myheartdr/common/articles.cfm?ARTID=187. 2004.
15. Stegemann, J. P., and R. M. Nerem. Phenotype modulation in vascular tissue engineering using biochemical and mechanical stimulation. Ann. Biomed. Eng. 31:391-402, 2003.
16. Stegemann, J. P., and R. M. Nerem. Altered response of vascular smooth muscle cells to exogenous biochemical stimulation in two- and three-dimensional culture. Exp. Cell Res. 283:146-155, 2003.
17. Taylor, P. M., P. Batten, N. J. Brand, P. S. Thomas, and M. H. Yacoub. The cardiac valve interstitial cell. Int. J. Biochem. Cell Biol. 35:113-118, 2003.
18. Warnock, J. N., S. Konduri, Z. He, and A. P. Yoganathan. Design of a sterile organ culture system for the ex vivo study of aortic heart valves. J. Biomech. Eng. 127:(in press), 2005.
19. Weber, K. T., Y. Sun, L. C. Katwa, J. P. Cleutjens, and G. Zhou. Connective tissue and repair in the heart. Potential regulatory mechanisms. Ann. N. Y. Acad. Sci. 752:286-299, 1995.
20. Weston, M. W., and A. P. Yoganathan. Biosynthetic activity in heart valve leaflets in response to in vitro flow environments. Ann. Biomed. Eng. 29:752-763, 2001.
21. Willems, I. E., M. G. Havenith, J. F. Smits, and M. J. Dae-men. Structural alterations in heart valves during left ventricular pressure overload in the rat. Lab. Invest. 71:127-133, 1994.
22. Xing, Y., J. N. Warnock, Z. He, S. Hilbert, and A. P. Yoganathan. Cyclic pressure affects the biological properties of porcine aortic valve leaflets in a magnitude- and frequency-dependent manner. Ann. Biomed. Eng. 32:2004.
23. Xing, Y., Z. He, J. N. Warnock, S. Hilbert, and A. P. Yoganathan. Effects of constant static pressure on the biological properties of porcine aortic valve leaflets. Ann. Biomed. Eng. 32:555-562, 2004.