A new scaleable method for the purification of botulinum neurotoxin type E

Journal of Biotechnology

Volumn 119, Issue 2, 2005, Pages 204-211

  Gessler, Frank ^a  

^a UNIVERSITY OF GÖTTINGEN   (Germany)

Author keywords

Botulinum neurotoxins; Chromatography; Clostridium botulinum; Microfiltration; Purification; Ultrafiltration

Indexed keywords

BACTERIA; CENTRIFUGATION; CHROMATOGRAPHIC ANALYSIS; DIALYSIS; DISEASES; FILTRATION; METABOLITES; MICROFILTRATION; NEUROLOGY; PURIFICATION;

BOTULINUM NEUROTOXIN; CLOSTRIDIUM BOTULINUM; NEURO-MUSCULAR DISORDERS; TOXINS;

TOXIC MATERIALS;

BOTULINUM TOXIN E;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BACTERIUM CULTURE; CENTRIFUGATION; CHROMATOGRAPHY; CONTROLLED STUDY; DIALYSIS; FILTRATION; METHODOLOGY; MOUSE; NONHUMAN; PRECIPITATION; PRIORITY JOURNAL; PRODUCT RECOVERY; PROTEIN PURIFICATION;

ANIMALS; BOTULINUM TOXINS; CHROMATOGRAPHY, ION EXCHANGE; CLOSTRIDIUM BOTULINUM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MICE; ULTRAFILTRATION;

BACTERIA (MICROORGANISMS); CLOSTRIDIUM BOTULINUM;

EID: 24044438473     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2005.03.013     Document Type: Article

References (20)

1. H. Böhnel, B. Schwagerick, and F. Gessler Visceral botulism - a new form of bovine Clostridium botulinum toxication J. Vet. Med. A 48 2001 373 383
2. D.S.J. Collier, S.O. Collier, and P.D. Rossdale Grass sickness - the same old suspects but still no convictions Equine Vet. J. 33 2001 540 542
3. B.R. DasGupta, and S. Rasmussen Purification and amino acid composition of type E botulinum neurotoxin Toxicon 21 1983 535 545
4. K.S. De Jongh, C.L. Schwartzkoff, and M.E.H. Howden Clostridium botulinum type D neurotoxin: purification and detection Toxicon 27 1989 221 228
5. F. Gessler, and H. Böhnel Production and purification of Clostridium botulinum type C and D neurotoxin FEMS Immunol. Med. Microbiol. 24 1999 361 367
6. J.A. Giménez, and G. Sugiyama Simplified purification method for Clostridium botulinum type E toxin Appl. Environ. Microbiol. 53 1987 2827 2830
7. Heukeshoven, J. and Dernick, R. (1986). In: Radola, B.J., (Ed.), Elektrophorese-Forum' 86. Autonomously published, 22-27.
8. S. Hielm, J. Björkroth, E. Hyytiä, and H. Korkeala Prevalence of Clostridium botulinum in Finnish trout farms: pulsed-field gel electrophoresis typing reveals extensive genetic diversity among type E isolates Appl. Environ. Microbiol. 64 1998 4161 4167
9. K.R. Kessler, and R. Benecke Botulinum toxin. From poison to remedy Neurotoxicology 18 1997 761 770
10. M. Kitamura, S. Sakaguchi, and G. Sakaguchi Purification and some properties of Clostridium botulinum type E toxin Biochim. Biophys. Acta 168 1968 207 217
11. O.P. Kreyden Botulinum toxin: from poison to pharmaceutical. The history of a poison that became useful to mankind Curr. Probl. Dermatol. 30 2002 94 100
12. C.C. Mechem, and F.G. Walter Wound botulism Vet. Human. Toxicol. 36 1994 233 237
13. T.F. Midura, and S.S. Arnon Infant botulism: Identification of Clostridium botulinum and its toxins in feces Lancet 2 1976 934 936
14. S. Prabaykaran, W. Tepp, and B.R. DasGupta Botulinum neurotoxin types B and E: purification, limited proteolysis by endoproteinase Glu-C and pepsin, and comparison of their identified cleaved sites relative to three-dimensional structure of type A neurotoxin Toxicon 39 2001 1515 1531
15. J.R. Rooney, and M.E. Prickett New puzzler for equine practitioners: shaker foal syndrome Mod. Vet. Pract. 48 1976 44 45
16. G. Sakaguchii Clostridium botulinum toxins F. Dorner J. Drews Pharmacology of Bacterial Toxins, International Encyclopedia of Pharmacology and Therapeutics 1986 Pergamon Press Oxford 519 548
17. J.J. Schmidt, and L.S. Siegel Purification of type E botulinum neurotoxin by high-performance ion exchange chromatography Anal. Biochem. 156 1986 213 219
18. Smith, L.D.S., Sugiyama, H., 1988. Botulism. The organism, its toxin, the disease. C.C. Thomas, Springfield.
19. S. Sugii, and G. Sakaguchii Botulogenic properties of vegetables with special reference to the molecular size of the toxin in them J. Food Saf. 1 1977 53 56
20. G.T. Weatherly, A. Bouvier, D.D. Lydiard, L. Chapline, I. Henderson, J.L. Schrimsher, and S.R. Shepard Initial purification of recombinant botulinum neurotoxin fragments for pharmaceutical production using hydrophobic charge induction chromatography J. Chromatogr. A 952 2002 99 110