Soluble methionine enhances accumulation of a 15 kDa zein, a methionine-rich storage protein, in transgenic alfalfa but not in transgenic tobacco plants

Journal of Experimental Botany

Volumn 56, Issue 419, 2005, Pages 2443-2452

  Amira, Golan ^a   Ifat, Matityahu ^a   Tal, Avraham ^a   Hana, Badani ^b   Shmuel, Galili ^b   Rachel, Amir ^a,c  

^a MIGAL GALILEE TECHNOLOGY CENTER   (Israel)

^b VOLCANI CENTER   (Israel)

^c TEL HAI COLLEGE   (Israel)

Author keywords

15 kDa zein; Amino acids; Cystathionine synthase; Forage plants; Methionine; Methionine rich storage protein; Nutritional quality; Transgenic plants

Indexed keywords

AMINO ACIDS; CELLS; PROTEINS; TISSUE; TOBACCO;

PLANT SPECIES; TOBACCO SUSPENSION CELLS;

PLANTS (BOTANY);

LYASE; METHIONINE; O SUCCINYLHOMOSERINE (THIOL) LYASE; O-SUCCINYLHOMOSERINE (THIOL)-LYASE; PRIMER DNA; ZEIN;

ALFALFA; ARTICLE; CELL CULTURE; COMPARATIVE STUDY; GENETICS; METABOLISM; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; SPECIES DIFFERENCE; TOBACCO; TRANSGENIC PLANT;

BASE SEQUENCE; CARBON-OXYGEN LYASES; CELLS, CULTURED; CLONING, MOLECULAR; DNA PRIMERS; MEDICAGO SATIVA; METHIONINE; PLANTS, GENETICALLY MODIFIED; SPECIES SPECIFICITY; TOBACCO; ZEIN;

ARABIDOPSIS; CAULIFLOWER MOSAIC VIRUS; MEDICAGO SATIVA; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM;

EID: 23944462177     PISSN: 00220957     EISSN: None     Source Type: Journal    
DOI: 10.1093/jxb/eri237     Document Type: Article

References (49)

1. Altenbach SB, Kuo CC, Staraci LC, Pearson KW, Wainwright C, Georgescu A. 1992. Accumulation of brazil nut albumin in seeds of transgenic canola results in enhanced levels of seed protein methionine. Plant Molecular Biology 18, 235-245.
2. Altenbach SB, Pearson KW, Meeker G, Staraci LC, Samuel SM. 1989. Enhancement of the methionine content of seed proteins by expression of a chimeric gene encoding a methionine-rich protein in transgenic plants. Plant Molecular Biology 13, 513-522.
3. Amir R, Galili G. 2003. Approaches to improve the nutritional values of transgenic plants by increasing their methionine content. In: Hemantaranjan A, ed. Advances in plant physiology, Vol. 6. Jodhpur: Scientific Publishers, 61-77.
4. Anthony J, Buhr D, Ronhovde G, Genovesi D, Lane T, Yingling R, Aves K, Rosato M, Anderson P. 1997. Transgenic maize with elevated 10 kDa zein and methionine. In: De Kok LDK, Cram WJ, Stulen I, Brunold C, Rennenberg H, eds. Sulfur metabolism in higher plants: molecular, ecophysiological and nutritional aspects. Leiden, The Netherlands: Backhuys Publishers, 295-297.
5. Avraham T, Badani H, Galili S, Amir R. 2005. Enhanced levels of methionine and cysteine in transgenic alfalfa (Medicago sativa L.) plants overexpressing the Arabidopsis cystathionine γ-synthase gene. Plant Biotechnology Journal 3, 71-80.
6. Bagga S, Adams H, Kemp JD, Sengupta-Gopalan C. 1995. Accumulation of 15-kilodalton zein in novel protein bodies in transgenic tobacco. Plant Physioliogy 107, 13-23.
7. Bagga S, Adams H, Rodriguez FD, Kemp JD, Sengupta-Gopalan C. 1997. Coexpression of the δ-maize zein and β-zein genes results in stable accumulation of δ-maize in endoplasmic reticulum drived protein bodies formed by β-zein. The Plant Cell 9, 1683-1696.
8. Bagga S, Armendaris A, Klypina N, Ray I, Ghoshroy S, Endress M, Sutton D, Kemp JD, Sengupta-Gopalan C. 2004. Genetic engineering ruminal stable high methionine protein in the foliage of alfalfa. Plant Science 166, 273-283.
9. Beach LR, Spencer D, Randall PJ, Higgins TJV. 1985. Transcriptional and post- transcriptional regulation of storage protein gene expressing in sulfur-deficient pea seeds. Nucleic Acids Research 13, 999-1013.
10. Bellucci M, Alpini A, Arcioni A. 2002. Zein accumulation in forage species (Lotus corniculatus and Medicago sativa) and co-expression of the γ-zein:KDEL and β-zein:KDEL polypeptides in tobacco leaf. Plant Cell Reports 20, 848-856.
11. Bellucci M, Alpini A, Paolocci F, Cong L, Arcioni A. 2000. Accumulation of maize γ-zein and γ-zein:KDEL to high levels in tobacco leaves and differential increase of Bip synthesis in transformants. Theoretical and Applied Genetics 101, 796-804.
12. Chiaiese P, Ohkama-Ohtsu N, Molvig L, Godfree R, Dove H, Hocart C, Fujiwara T, Higgins TJ, Tabe LM. 2004. Sulphur and nitrogen nutrition influence the response of chickpea seeds to an added, transgenic sink for organic sulphur. Journal of Experimental Botany 55, 1889-1901.
13. Christiansen P, Gibson JM, Moore A, Pedersen C, Tabe L, Larkin PJ. 2000. Transgenic Trifolium repens with foliage accumulating the high sulphur protein, sunflower seed albumin. Transgenic Research 9, 103-113.
14. Di R, Kim J, Martin MN, Leustek T, Jhoo J, Ho CT, Tumer NE. 2003. Enhancement of the primary flavor compound methional in potato by increasing the level of soluble methionine. Journal of Agriculture and Food Chemistry 51, 5695-5702.
15. Droux M, Gakiere B, Denis L, Ravanel S, Tabe L, Lappartient AG, Job D. 2000. Methionine biosynthesis in plants: biochemical and regulatory aspects. In: C. Brunold, ed. Sulfur nutrition and sulfur assimilation in higher plants. Bern, Switzerland: Paul Haupt, 73-92.
16. Ealing P, Hancock KR, White DWR. 1994. Expression of the pea albumin 1 gene in transgenic white clover and tobacco. Transgenic Research 3, 344-354.
17. Frankard V, Ghislain M, Negrutiu I, Jacobs M. 1991. High threonine producer mutant of Nicotiana sylvestris (Spegazzini. and Comes). Theoretical and Applied Genetics 82, 273-282.
18. Habben JE, Larkins BA. 1995. Genetic modification of seed proteins. Current Opinion in Biotechnology 6, 171-174.
19. Hacham Y, Avraham T, Amir R. 2002. The N-terminal region of Arabidopsis cystathionine gamma synthase plays an important role in methionine metabolism. Plant Physiology 128, 454-462.
20. Hadjukiewicz P, Svab Z, Maliga P. 1994. The small, versatile pPZP family of Agrobacterium binary vectors for plant transformation. Plant Molecular Biology 25, 989-994.
21. Hagan ND, Upadhyaya N, Tabe LM, Higgins TJ. 2003. The redistribution of protein sulfur in transgenic rice expressing a gene for a foreign, sulfur-rich protein. The Plant Journal 34, 1-11.
22. Heremans B, Jacobs M. 1997. A mutant of Arabidopsis thaliana (L.) Heynh. with modified control of aspartate kinase by threonine. Biochemical Genetics 35, 139-153.
23. Hesse H, Hoefgen R. 2004. Molecular aspects of methionine biosynthesis. Trends in Plant Science 8, 259-262.
24. Hirai MY, Fujiwara TF, Chino TM, Naito S. 1995. Effects of sulfate concentrations on the expression of a soybean seed storage protein gene and its reversibility in transgenic Arabidopsis thaliana. Plant and Cell Physiology 36, 1331-1339.
25. Hoffman L, Donaldson DD, Bookland R, Rashka K, Herman EM. 1987. Synthesis and protein body deposition of maize 15 kD zein in transgenic tobacco seeds. The EMBO Journal 6, 3213-3221.
26. Horsch RB, Fry J, Hoffmann NL, Eichholtz D, Rogers SG, Fraley R. 1985. A simple and general method for transferring genes into plants. Science 227, 1229-1231.
27. Galili G, Galili S, Lewinsohn E, Tadmor Y. 2002. Genetic, molecular and genomic approaches to improve the value of plant foods and feeds. Critical Reviews in Plant Sciences 21, 167-204.
28. Galili S, Guenoune D, Wilinger S, Kapulnic Y. 2000. Enhanced levels of free and protein bound threonine in transgenic alfalfa (Medicago sativa L.) expressing a bacterial feed back insensitive aspartate kinase gene. Transgenic Research 9, 137-144.
29. Guenoune D, Amir R, Badani H, Wolf S, Galili S. 2003. Coexpression of the soybean vegetative storage protein beta subunit (S-VSPβ) either with the bacterial feedback-insensitive dihydrodipicolinate synthase or with S-VSPα stabilizes the S-VSPβ transgene protein and enhances lysine production in transgenic tobacco plants. Transgenic Research 12, 123-126.
30. Jung R, Martino-Catt S, Townsend J, Beach L. 1997. Expression of a sulfur rich protein in soybean seeds causes an altered seed protein composition. Proceedings of the Fifth International Congress of Plant Molecular Biology, Singapore. The Netherlands: Kluwer Academic Publishers.
31. Khan MR, Ceriotti A, Tabe L, Aryan A, McNabb W, Moore A, Craig S, Spencer D, Higgins TJ. 1996. Accumulation of a sulphur-rich seed albumin from sunflower in the leaves of transgenic subterranean clover (Trifolium subterraneum L.). Transgenic Research 5, 179-185.
32. Kim J, Lee M, Chalam R, Martin M, Leustek T, Boerjan W. 2002. Constitutive overexpression of cystathionine γ-synthase in Arabidopsis thaliana leads to accumulation of soluble methionine and S-methylmethionine. Plant Physiology 128, 95-107.
33. Kreft O, Hoefgen R, Hesse H. 2003. Functional analysis of cystathionine gamma-synthase in genetically engineered potato plants. Plant Physiology 131, 1843-1854.
34. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
35. Lee TT, Wang MM, Hou RC, Chen LJ, Su RC, Wang CS, Tzen JT. 2003. Enhanced methionine and cysteine levels in transgenic rice seeds by the accumulation of sesame 2S albumin. Bioscience, Biotechnology and Biochemistry 67, 1699-1705.
36. Molvig L, Tabe LM, Eggum BO, Moore A, Craig S, Spencer D, Higgins TJV. 1997. Enhanced methionine level and increased nutritive value of seeds of transgenic lupins (Lupinus angustifolius L.) expressing a sunflower seed albumin gene. Proceedings of the National Academy of Sciences, USA 94, 8393-8398.
37. Muntz K, Christov V, Jung R, Saalbach G, Saalbach I, Waddell D, Pickardt T, Schieder O. 1997. Genetic engineering of high methionine proteins in grain legumes. In: De Kok LDK, Cram WJ, Stulen I, Brunold C, Rennenberg H, eds. Sulfur metabolism in higher plants: molecular, ecophysiological and nutritional aspects. Leiden, The Netherlands: Backhuys Publishers, 295-297.
38. Naito S, Inaba-Higano K, Kumagai T, Kanno T, Nambara E, Fujiwara T, Chino M, Komeda Y. 1994. Maternal effects of mto1 mutation, that causes overaccumulation of soluble methionine, on the expression of soybean beta-conglycinin gene promoter-GUS fusion in transgenic Arabidopsis thaliana. Plant and Cell Physiology 35, 1057-1063.
39. Pickering FS, Reis PJ. 1993. Effects of abomasal supplements of methionine on wool growth of grazing sheep. Australian Journal of Experimental Agriculture 33, 7-12.
40. Sharma SB, Hancock KR, Ealing PM, White DWR. 1998. Expression of a sulfur-rich maize seed storage protein, deltazein, in white clover (Trifolium repens) to improve forage quality. Molecular Breeding 4, 435-448.
41. Tabe LM, Droux M. 2001. Sulfur assimilation in developing lupin cotyledons could contribute significant to the accumulation of organic sulfur reserves in the seed. Plant Physiology 126, 176-187.
42. Tabe LM, Droux M. 2002. Limits to sulfur accumulation in transgenic lupin seeds expressing a foreign sulfur-rich protein. Plant Physiology 128, 1137-1148.
43. Tabe LM, Higgins TJV. 1998. Engineering plant protein composition for improved nutrition. Trends in Plant Science 3, 282-286.
44. Tabe LM, Wardley-Richardson T, Ceriotti A, Aryan A, Mcnabb W, Moore A, Higgins TJV. 1995. A biotechnological approach to improving the nutritive value of alfalfa. Journal of Animal Science 73, 2752-2759.
45. Tang G, Zhu X, Tang X, Galili G. 2000. A novel composite locus of Arabidopsis encoding two polypeptides with metabolically related but distinct functions in lysine catabolism. The Plant Journal 23, 195-203.
46. Takeda Y, Hirokawa H, Nagata T. 1992. The replication origin of proplastid DNA in cultured cells of tobacco. Molecular and General Genetics 232, 191-198.
47. Verwoerd TC, van Paridon PA, van Ooyen AJ, van Lent JW, Hoekema A, Pen J. 1995. Stable accumulation of Aspergillus niger phytase in transgenic tobacco leaves. Plant Physiology 109, 1199-205.
48. Wandelt CI, Rafiqul M, Khan I, Craig S, Schroeder HE, Spencer D, Higgins TJV. 1992. Vicilin with carboxy terminal KDEL is retained in the endoplasmic reticulum and accumulates to high levels in the leaves of transgenic plants. The Plant Journal 2, 181-192.
49. Xu S, Harrison JH, Chalupa W, Sniffen C, Julien W, Sato H, Fujieda T, Watanabe K, Ueda T, Suzuki H. 1998. Effects of ruminal bypass lysine and methionine on milk yield and composition of lactating cows. Dairy Science 81, 1062-1077.