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Volumn 1, Issue 1, 2005, Pages 64-69

Synthesis of covalent DNA-protein conjugates by expressed protein ligation

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN; CYSTEINE; DNA; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; INTEIN; MALTOSE BINDING PROTEIN; MALTOSE-BINDING PROTEIN; N HYDROXYSUCCINIMIDE; N-HYDROXYSUCCINIMIDE; OLIGONUCLEOTIDE; SUCCINIMIDE DERIVATIVE;

EID: 23944457508     PISSN: 1742206X     EISSN: 17422051     Source Type: Journal    
DOI: 10.1039/b503839a     Document Type: Article
Times cited : (32)

References (17)
  • 11
    • 0037425124 scopus 로고    scopus 로고
    • Universal cysteine modifiers, such as 1, for the functionalization of DNA oligomers and their use in EPL: C.M. Niemeyer, M. Lovrinovic, C. Deutsch, German patent application, priority date, October 30th, 2003. During the preparation of this manuscript, Takeda and coworkers reported on the conjugation of DNA oligonucleotides to the C-terminus of a green fluorescent protein variant, ECFP, by EPL using a cysteine-derivatized isonipecotic acid-NHS ester (
    • M. Lovrinovic R. Seidel R. Wacker H. Schroeder O. Seitz M. Engelhard R. Goody C. M. Niemeyer Chem. Commun. 2003 822 823
    • (2003) Chem. Commun. , pp. 822-823
    • Lovrinovic, M.1    Seidel, R.2    Wacker, R.3    Schroeder, H.4    Seitz, O.5    Engelhard, M.6    Goody, R.7    Niemeyer, C.M.8
  • 12
    • 1942438549 scopus 로고    scopus 로고
    • ). However, no functionality of the conjugated protein was demonstrated. Very recently, dsDNA-protein conjugates were prepared by EPL and used in a PCR-based immunoassay:
    • S. Takeda S. Tsukiji T. Nagamune Bioorg. Med. Chem. Lett. 2004 14 2407 2410
    • (2004) Bioorg. Med. Chem. Lett. , vol.14 , pp. 2407-2410
    • Takeda, S.1    Tsukiji, S.2    Nagamune, T.3
  • 17
    • 33644803734 scopus 로고    scopus 로고
    • The DNA-EYFP conjugates were purified by anion-exchange HPLC (Nucleosil 4000-7 PEI, Macherey-Nagel) by gradually increasing the NaCl concentration from 0 to 0.7 M. The fluorescence of the conjugate was detected using a 515 nm excitation and a 527 nm emission filter, thereby clearly indicating the biological activity of the protein component
    • The DNA-EYFP conjugates were purified by anion-exchange HPLC (Nucleosil 4000-7 PEI, Macherey-Nagel) by gradually increasing the NaCl concentration from 0 to 0.7 M. The fluorescence of the conjugate was detected using a 515 nm excitation and a 527 nm emission filter, thereby clearly indicating the biological activity of the protein component


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.