Isolation and characterization of a nitrate reductase deficient mutant of Chlorella ellipsoidea (Chlorophyta)

Journal of Applied Phycology

Volumn 17, Issue 4, 2005, Pages 281-286

  Wang, Yiqin ^a   Chen, Yan ^b   Zhang, Xiaoyu ^b   Wang, Peng ^a   Geng, Degui ^a   Zhao, Shimin ^a   Zhang, Liming ^a   Sun, Yongru ^a  

^a INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY   (China)

^b BEIJING FORESTRY UNIVERSITY   (China)

Author keywords

Chlorella ellipsoidea; Complementation experiment; Mutant analysis; Nitrate reductase deficient mutant

Indexed keywords

ALGA;

'CHLORELLA' ELLIPSOIDEA; ALGAE; CHLORELLA (CHLORELLALES); CHLORELLA (PRASIOLALES); CHLORELLA (UNCLASSIFIED CHLOROPHYCEAE); CHLORELLA (UNCLASSIFIED TREBOUXIOPHYCEAE);

EID: 23844539021     PISSN: 09218971     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10811-005-5559-7     Document Type: Article

References (16)

1. Ahmad I, Hellebust JA (1990) Glutamine synthetase isoenzymes in the green soil alga Stichococcus bacillaris Naeg. Plant Physiol. 83: 259-261.
2. Brown J, Small IS, Wray JL (1981) Age dependent conversion of nitrate reductase to cytochrome c reductase species in barley leaf extracts. Phytochemistry 20: 389-398.
3. Buchanan RJ, Wray JJ (1982) Isolation of molybdenum cofactor defective cell lines of Nicoiianum tobacum cultivar xanthi. Mol. Gen. Genet. 189: 240-244.
4. Campbell WH, Kinghorn JR (1990) Functional domains of assimilatory nitrate reductase and nitrite reductase. Trends Biochem. Sci. 15: 315-319.
5. Chen Y, Wang YQ, Zhang LM, Sun YR, Li WB (2001) Highly efficient expression of rabbit neutrophil peptide-1 gene in Chlorella ellipsoidea cells. Curr. Genet. 39: 365-370.
6. Dawson HN, Burlingame R, Cannons AC (1997) Stable transformation of Chlorella: Rescue of nitrate reductase-deficient mutants with the nitrate reductase gene. Curr. Microbiol. 35: 356-362.
7. Dawson HN, Pendleton LC, Solomonson LP, Cannons AC (1996) Cloning and characterization of the NR-encoding gene from Chlorella vulgaris: Structure and identification of transcription start points and initiator sequences. Gene. 171: 139-145.
8. Jarvis EE, Brown LM (1991) Transient expression of firefly luciferase in protoplast of the green alga Chlorella ellipsoidea. Curr. Genet. 19: 317-321.
9. Knobloch O, Tischner R (1989) Characterization of nitrate reductase of deficient mutants of Chlorella sorokiniana. Plant Physiol. 89: 786-791.
10. Maruyama M, Horakova I, Honda H, Xing XH, Shiragimi N, Unno H (1994) Introduction of foreign DNA into Chlorella saccharophila by electroporation. Biotechnol. Tech. 8: 821-826.
11. Oostindier-Braaksma FJ, Feenstra WJ (1973) Isolation and characterization of chlorate-resistant mutants of Arabidopsis thaliana. Mut. Res. 19: 175-185.
12. Richard L, Hawkins, Nakamura M. (1999) Expression of growth hormone by the eukaryotic alga, Chlorella. Curr. Microbiol. 38: 335-341.
13. Solomonson LP, Barber MJ (1990) Assimilatory nitrate reductase: Functional properties and regulation. Annu. Rev. Plant Physiol. Plant Mol. Biol. 41: 240-253.
14. Tischner R (1976) Zur Induktion der Nitratreduktase und Nitritreduktase in vollsynchronen Chlorella Kulturen. Planta 132: 285-290.
15. Wang P, Li WB, Sun YR, Wang YQ (2004) Rapid isolation and functional analysis of promoter sequences of the nitrate reductase gene from Chlorella ellipsoidea. J. appl. Phycol. 16: 11-16.
16. Wang P, Wang YQ, Geng DG, Li WB, Sun YR (2003) Molecular cloning and characterization of a cDNA encoding nitrate reductase from Chlorella ellipsoidea (Chlorophyta). J. appl. Phycol. 15: 457-463.