16S rDNA PCR and denaturing gradient gel electrophoresis; a single generic test for detecting and differentiating Mycoplasma species

Journal of Medical Microbiology

Volumn 54, Issue 8, 2005, Pages 731-739

  McAuliffe, Laura ^a   Ellis, Richard J ^b   Lawes, Jo R ^a   Ayling, Roger D ^a   Nicholas, Robin A J ^a  

^a VETERINARY LABORATORIES AGENCY   (United Kingdom)

^b IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DNA 16S;

ARTICLE; BACTERIAL INFECTION; BACTERIAL STRAIN; BACTERIUM DETECTION; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA EXTRACTION; MYCOPLASMA; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEROLOGY;

ANIMALS; DNA, RIBOSOMAL; ELECTROPHORESIS; HUMANS; MYCOPLASMA; MYCOPLASMA INFECTIONS; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S;

EID: 23744507374     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/jmm.0.46058-0     Document Type: Article

References (17)

1. Ashelford, K. E., Weightman, A. J. & Fry, J. C. (2002). PRIMROSE: a computer program for generating and estimating the phylogenetic range of 16S rRNA oligonucleotide probes and primers in conjunction with the RDP-II database. Nucleic Acids Res 30, 3481-3489.
2. Chalker, V. J. & Brownlie, J. (2004). Taxonomy of the canine Mollicutes by 16S rRNA gene and 16S/23S rRNA intergenic spacer region sequence comparison. Int J Syst Evol Microbiol 54, 537-542.
3. Domann, E., Hong, G., Imirzalioglu, C., Turschner, S., Kuhle, J., Watzel, C., Hain, T., Hossain, H. & Chakraborty, T. (2003). Culture-independent identification of pathogenic bacteria and polymicrobial infections in the genitourinary tract of renal transplant recipients. J Clin Microbiol 41, 5500-5510.
4. Fischer, S. G. & Lerman, L. S. (1983). DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with theory. Proc Natl Acad Sci U S A 80, 1579-1583.
5. Heldtander, M., Wesonga, H., Bolske, G., Pettersson, B. & Johansson, K. E. (2001). Genetic diversity and evolution of Mycoplasma capricolum subsp. capripneumoniae strains from eastern Africa assessed by 16S rDNA sequence analysis. Vet Microbiol 78, 13-28.
6. Hurtle, W., Bode, E., Kaplan, R. S., Garrison, J., Kearney, B., Shoemaker, D., Henchal, E. & Norwood, D. (2003). Use of denaturing high-performance liquid chromatography to identify Bacillus anthracis by analysis of the 16S-23S rRNA interspacer region and gyrA gene. J Clin Microbiol 41, 4758-4766.
7. Jensen, J. S., Borre, M. B. & Dohn, B. (2003). Detection of Mycoplasma genitalium by PCR amplification of the 16S rRNA gene. J Clin Microbiol 41, 261-266.
8. Lerman, L. S. & Beldjord, C. (1999). Comprehensive mutation detection with denaturing gradient gel electrophoresis. In Mutation Detection, pp. 35-61. Edited by R. G. H. Cotton, E. Edkins and S. Forrest. New York: Oxford University Press.
9. McAuliffe, L., Ellis, R. J., Ayling, R. D. & Nicholas, R. A. (2003). Differentiation of Mycoplasma species by 16S ribosomal DNA PCR and denaturing gradient gel electrophoresis fingerprinting. J Clin Microbiol 41, 4844-4847.
10. Muyzer, G. (1999). DGGE/TGGE: a method for identifying genes from natural ecosystems. Curr Opin Microbiol 2, 317-322.
11. Muyzer, G., de Waal, E. C. & Uitterlinden, A. G. (1993). Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 59, 695-700.
12. Nicholas, R. & Baker, S. (1998). Recovery of mycoplasmas from animals. Methods Mol Biol 104, 37-43.
13. Ogilvie, T. H., Rosendal, S., Blackwell, T. E., Rostkowski, C. M., Julian, R. J. & Ruhnke, L. (1983). Mycoplasma felis as a cause of pleuritis in horses. J Am Vet Med Assoc 182, 1374-1376.
14. Pettersson, B., Leitner, T., Ronaghi, M., Bolske, G., Uhlen, M. & Johansson, K. E. (1996). Phylogeny of the Mycoplasma mycoides cluster as determined by sequence analysis of the 16S rRNA genes from the two rRNA operons. J Bacteriol 178, 4131-4142.
15. Pettersson, B., Tully, J. G., Bolske, G. & Johansson, K. E. (2001). Re-evaluation of the classical Mycoplasma lipophilum cluster (Weisburg et al. 1989) and description of two new clusters in the hominis group based on 16S rDNA sequences. Int J Syst Evol Microbiol 51, 633-643.
16. Stellrecht, K. A., Woron, A. M., Mishrik, N. G. & Venezia, R. A. (2004). Comparison of multiplex PCR assay with culture for detection of genital mycoplasmas. J Clin Microbiol 42, 1528-1533.
17. Wang, H., Kong, F., Jelfs, P., James, G. & Gilbert, G. L. (2004). Simultaneous detection and identification of common cell culture contaminant and pathogenic mollicutes strains by reverse line blot hybridization. Appl Environ Microbiol 70, 1483-1486.