Mutational analysis of the energetics of the GrpE·DnaK binding interface: Equilibrium association constants by sedimentation velocity analytical ultracentrifugation

Journal of Molecular Biology

Volumn 339, Issue 2, 2004, Pages 447-458

  Gelinas, Amy D ^a   Toth, Joseph ^a   Bethoney, Kelley A ^a   Stafford, Walter F ^a   Harrison, Celia J ^a  

^a BOSTON BIOMEDICAL RESEARCH INSTITUTE   (United States)

Author keywords

alanine scanning mutagenesis; analytical ultracentrifugation; AUC, analytical ultracentrifugation; equilibrium association constants; molecular chaperone; protein protein interaction; s, sedimentation coefficient

Indexed keywords

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATASE; ALANINE; ARGININE; BARNASE; BARSTAR; HEAT SHOCK PROTEIN; HEAT SHOCK PROTEIN GRPE; PROTEIN DNAK; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONCENTRATION RESPONSE; DISSOCIATION CONSTANT; ELECTRICITY; ENERGY; EQUILIBRIUM CONSTANT; GENE MUTATION; MOLECULAR DYNAMICS; MUTAGENESIS; MUTATIONAL ANALYSIS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; SEDIMENTATION RATE; THERMODYNAMICS; THERMORECEPTOR; ULTRACENTRIFUGATION;

BACTERIA (MICROORGANISMS); PROKARYOTA;

EID: 2342525163     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2004.03.074     Document Type: Article

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