An efficient protocol for the identification of protein phosphorylation in a seedless plant, sensitive enough to detect members of signalling cascades

Electrophoresis

Volumn 25, Issue 7-8, 2004, Pages 1149-1159

  Heintz, Dimitri ^a   Wurtz, Virginie ^b   High, Anthony A ^b   Van Dorsselaer, Alain ^b   Reski, Ralf ^a   Sarnighausen, Eric ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

^b Lab Spectrom Masse Bio Organique   (France)

Author keywords

Immobilized metal affinity chromatography; Mass spectrometry; Moss; Phosphorylation; Physcomitrella patens; Proteomics

Indexed keywords

AFFINITY CHROMATOGRAPHY; CAPILLARY ELECTROPHORESIS; INDUCTIVELY COUPLED PLASMA; MOLECULAR BIOLOGY; PEPTIDES; PHOSPHATASES; PHOSPHORYLATION; SIGNAL TRANSDUCTION; TRANSCRIPTION;

FE 3+; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; MOSS; PHOSPHOPEPTIDES; PHOSPHORYLATED PEPTIDES; PHYSCOMITRELLA PATENS; PROTEIN EXTRACT; PROTEOMICS; REVERSE-PHASE CHROMATOGRAPHY; TOTAL PROTEIN;

MASS SPECTROMETRY;

ALKALINE PHOSPHATASE; FERROUS ION; NANOPARTICLE; PHOSPHOPEPTIDE; PLANT EXTRACT; TRANSCRIPTION FACTOR;

ARTICLE; CAPILLARY ZONE ELECTROPHORESIS; CONTROLLED STUDY; COVALENT BOND; DESALINATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOSS; NONHUMAN; PHYSCOMITRELLA PATENS; PHYTOCHEMISTRY; PLANT SEED; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN DEPHOSPHORYLATION; PROTEIN HYDROLYSIS; PROTEIN ISOLATION; PROTEIN PHOSPHORYLATION; PROTEOMICS; REPRODUCIBILITY; REVERSED PHASE LIQUID CHROMATOGRAPHY; SENSITIVITY ANALYSIS; SIGNAL TRANSDUCTION; TANDEM MASS SPECTROMETRY; TWO DIMENSIONAL GEL ELECTROPHORESIS;

PHYSCOMITRELLA PATENS;

EID: 2342518184     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.200305795     Document Type: Article

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