Subcutaneous Adipocytes Can Differentiate into Bone-Forming Cells in Vitro and in Vivo

Tissue Engineering

Volumn 10, Issue 3-4, 2004, Pages 381-391

  Justesen, Jeannette ^a   Pedersen, Steen B ^a   Stenderup, Karin ^a   Kassem, Moustapha ^a,b  

^a AARHUS UNIVERSITY HOSPITAL   (Denmark)

^b ODENSE UNIVERSITY HOSPITAL   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

4 (2 HYDROXYETHYL) 1 PIPERAZINEETHANESULFONIC ACID; ALKALINE PHOSPHATASE; ASCORBIC ACID; BIOTIN; CALCITRIOL; CALCIUM PHOSPHATE; DEXAMETHASONE; GLYCEROL 2 PHOSPHATE; HYDROXYAPATITE; INSULIN; ISOBUTYLMETHYLXANTHINE; LIOTHYRONINE; LIPOPROTEIN LIPASE; OSTEOCALCIN; PANTOTHENIC ACID; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA; TRANSCRIPTION FACTOR RUNX2;

ADIPOCYTE; ANIMAL EXPERIMENT; ANIMAL TISSUE; BONE DEFECT; CELL DIFFERENTIATION; CONFERENCE PAPER; CONTROLLED STUDY; CULTURE MEDIUM; CYTOCHEMISTRY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMPLANTATION; INCUBATION TIME; MOUSE; NONHUMAN; OSSIFICATION; OSTEOBLAST; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROADIPOCYTE; PSEUDARTHROSIS; SUBCUTANEOUS FAT;

ADIPOCYTES; ANIMALS; BONE AND BONES; CELL DIFFERENTIATION; CULTURE MEDIA; HUMANS; MICE; OSTEOBLASTS;

EID: 2342511492     PISSN: 10763279     EISSN: None     Source Type: Journal    
DOI: 10.1089/107632704323061744     Document Type: Conference Paper

References (38)

1. Rickard, D.J., Kassem, M., Hefferan, T.E., Sarkar, G., Spelsberg, T.C., and Riggs, B.L. Isolation and characterization of osteoblast precursor cells from human bone marrow. J. Bone Miner. Res. 11, 312, 1996.
2. Pittenger, M.F., Mackay, A.M., Beck, S.C., Jaiswal, R.K., Douglas, R., Mosca, J.D., Moorman, M.A., Simonetti, D.W., Craig, S., and Marshak, D.R. Multilineage potential of adult human mesenchymal stem cells. Science 284, 143, 1999.
3. Justesen, J., Stenderup, K., Eriksen, E., and Kassem, M. Maintenance of osteoblastic and adipocytic differentiation potential with age and osteoporosis in human marrow stromal cell cultures. Calcif. Tissue Int. 71, 36, 2002.
4. Park, S.R., Oreffo, R.O., and Triffitt, J.T. Interconversion potential of cloned human marrow adipocytes in vitro. Bone 24, 549, 1999.
5. Bennett, J.H., Joyner, C.J., Triffitt, J.T., and Owen, M.E. Adipocytic cells cultured from marrow have osteogenic potential. J. Cell Sci. 99, 131, 1991.
6. Beresford, J.N., Bennett, J.H., Devlin, C., Leboy, P.S., and Owen, M.E. Evidence for an inverse relationship between the differentiation of adipocytic and osteogenic cells in rat marrow stromal cell cultures. J. Cell Sci. 102, 341, 1992.
7. Gimble, J.M., Dorheim, M.A., Cheng, Q., Pekala, P., Enerback, S., Ellingsworth, L., Kincade, P.W., and Wang, C.S. Response of bone marrow stromal cells to adipogenic antagonists. Mol. Cell. Biol. 57, 4587, 1989.
8. Bellows, C.G., Wang, Y.H., Heersche, J.N., and Aubin, J.E. 1,25-Dihydroxyvitamin D3 stimulates adipocyte differentiation in cultures of fetal rat calvaria cells: Comparison with the effects of dexamethasone. Endocrinology 134, 2221, 1994.
9. Gimble, J.M., Morgan, C., Kelly, K., Wu, X., Dandapani, V., Wang, C.S., and Rosen, V. Bone morphogenetic proteins inhibit adipocyte differentiation by bone marrow stromal cells. J. Cell. Biochem. 58, 393, 1995.
10. Chen, T.L., Shen, W.J., and Kraemer, F.B. Human BMP-7/OP-I induces the growth and differentiation of adipocytes and osteoblasts in bone marrow stromal cell cultures. J. Cell. Biochem. 82, 187, 2001.
11. Tavassoli, M. Marrow adipose cells and hemopoiesis: An interpretative review. Exp. Hematol. 12, 139, 1984.
12. Bathija, A., Davis, S., and Trubowitz, S. Bone marrow adipose tissue: Response to acute starvation. Am. J. Hematol. 6, 191, 1979.
13. Greenberger, J.S. Corticosteroid-dependent differentiation of human marrow preadipocytes in vitro. In Vitro 15, 823, 1979.
14. Lanotte, M., Scott, D., Dexter, T.M., and Allen, T.D. Clonal preadipocyte cell lines with different phenotypes derived from murine marrow stroma: Factors influencing growth and adipogenesis in vitro. J. Cell. Physiol. 111, 177, 1982.
15. Halvorsen, Y.C., Wilkison, W.O., and Gimble, J.M. Adipose-derived stromal cells - their utility and potential in bone formation. Int. J. Obes. Relat. Metab. Disord. 24(Suppl. 4), S41, 2000.
16. Zuk, P.A., Zhu, M., Mizuno, H., Huang, J., Futrell, J.W., Katz, A.J., Benhaim, P., Lorenz, H.P., and Hedrick, M.H. Multilineage cells from human adipose tissue: Implications for cell-based therapies. Tissue Eng. 7, 211, 2001.
17. Halvorsen, Y.D., Franklin, D., Bond, A.L., Hitt, D.C., Auchter, C., Boskey, A.L., Paschalis, E.P., Wilkison, W.O., and Gimble, J.M. Extracellular matrix mineralization and osteoblast gene expression by human adipose tissue-derived stromal cells. Tissue Eng. 7, 729, 2001.
18. Hauner, H., Entenmann, G., Wabitsch, M., Gaillard, D., Ailhaud, G., Negrel, R., and Pfeiffer, E.F. Promoting effect of glucocorticoids on the differentiation of human adipocyte precursor cells cultured in a chemically defined medium. J. Clin. Invest. 84, 1663, 1989.
19. Sottile, V., and Seuwen, K. Bone morphogenetic protein-2 stimulates adipogenic differentiation of mesenchymal precursor cells in synergy with BRL 49653 (rosiglitazone). FEBS Lett. 475, 201, 2000.
20. Pedersen, S.B., Bruun, J.M., Kristensen, K., and Richelsen, B. Regulation of UCP1, UCP2, and UCP3 mRNA expression in brown adipose tissue, white adipose tissue, and skeletal muscle in rats by estrogen. Biochem. Biophys. Res. Commun. 288, 191, 2001.
21. Simonsen, J., Rosada, C., Serakinci, N., Justesen J, Stenderup, K., Rattan, S.I., and Kassem, M. Telomerase expression extends proliferative life span and prevents senescence-associated impairment of human osteoblast functions. Nat. Biotechnol. 20, 592, 2002.
22. Londos, C., Gruia-Gray, J., Brasaemle, D.L., Rondinone, C.M., Takeda, T., Dwyer, N.K., Barber, T., Kimmel, A.R., and Blanchette-Mackie, E.J. Perilipin: Possible roles in structure and metabolism of intracellular neutral lipids in adipocytes and steroidogenic cells. Int. J. Obes. Relat. Metab. Disord. 20(Suppl. 3), S97, 1996.
23. McManaman, J.L., Zabaronick, W., Schaack, J., and Orlicky, D.J. Lipid droplet targeting domains of adipophilin. J. Lipid Res. 44, 668, 2003.
24. Greenspan, P., Mayer, E.P., and Fowler, S.D. Nile red: A selective fluorescent stain for intracellular lipid droplets. J. Cell Biol. 100, 965, 1985.
25. Sackett, D.L., and Wolff, J. Nile red as a polarity-sensitive fluorescent probe of hydrophobic protein surfaces. Anal. Biochem. 167, 228, 1987.
26. Leboy, P.S., Beresford, J.N., Devlin, C., and Owen, M.E. Dexamethasone induction of osteoblast mRNAs in rat marrow stromal cell cultures. J. Cell. Physiol. 146, 370, 1991.
27. Kassem, M., Brixen, K., Blum, W.F., Mosekilde, L., and Eriksen, E.F. Normal osteoclastic and osteoblastic responses to exogenous growth hormone in patients with postmenopausal spinal osteoporosis. J. Bone Miner. Res. 9, 1365, 1994.
28. Green, E., Todd, B., and Heath, D. Mechanism of glucocorticoid regulation of alkaline phosphatase gene expression in osteoblast-like cells. Eur. J. Biochem. 188, 147, 1990.
29. Subramaniam, M., Colvard, D., Keeting, P.E., Rasmussen, K., Riggs, B.L., and Spelsberg, T.C. Glucocorticoid regulation of alkaline phosphatase, osteocalcin, and proto-oncogenes in normal human osteoblast-like cells. J. Cell. Biochem. 50, 411, 1992.
30. Lehmann, J.M., Moore, L.B., Smith-Oliver, T.A., Wilkison, W.O., Willson, T.M., and Kliewer, S.A. An antidiabetic thiazolidinedione is a high affinity ligand for peroxisome proliferator-activated receptor γ (PPAR γ). J. Biol. Chem. 270, 12953, 1995.
31. Adams, M., Montague, C.T., Prins, J.B., Holder, J.C., Smith, S.A., Sanders, L., Digby, J.E., Sewter, C.P., Lazar, M.A., Chatterjee, V.K., and O'Rahilly, S. Activators of peroxisome proliferator-activated receptor γ have depot-specific effects on human preadipocyte differentiation. J. Clin. Invest. 100, 3149, 1997.
32. Spiegelman, B.M. PPAR-γ: Adipogenic regulator and thiazolidinedione receptor. Diabetes 47, 507, 1998.
33. Mandrup, S., Loftus, T.M., MacDougald, O.A., Kuhajda, F.P., and Lane, M.D. Obese gene expression at in vivo levels by fat pads derived from s.c. implanted 3T3-F442A preadipocytes. Proc. Natl. Acad. Sci. U.S.A. 94, 4300,1997.
34. Safford, K., Hicok, K., Safford, S., Halvorsen, Y., Wilkinson, W., Gimble, J., and Rice, H. Neurogenic differentiation of murine and human adipose-derived stromal cells. Biochem. Biophys. Res. Commun. 294, 371, 2002.
35. Blanco, P., and Gehron, R.P. Marrow stromal stem cells. J Clin. Invest. 105, 1663, 2000.
36. Kuznetsov, S.A., Mankani, M.H., Gronthos, S., Satomura, K., Blanco, P., and Robey, P.G. Circulating skeletal stem cells. J. Cell Biol. 153, 1133, 2001.
37. Rosada, C., Justesen, J., Melsvik, D., Ebbesen, P., and Kassem, M. The human umbilical cord blood: A potential source for osteoblast progenitor cells. Calcif. Tissue Int. 72, 135, 2003.
38. Friedenstein, A. Osteogenic stem cells in bone marrow. Bone Miner. 7, 243, 1991.