Provision of epstein-barr virus-transformed B-cell lines in a routine tissue typing laboratory: Practicalities and applications

European Journal of Immunogenetics

Volumn 31, Issue 2, 2004, Pages 87-92

  Bass, H ^a   Walters, R ^a   Darke, C ^a  

^a Welsh Blood Service   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

HEPATITIS B ANTIGEN; VIRUS ANTIBODY;

ARTICLE; B LYMPHOCYTE; BACTERIUM CONTAMINATION; BLOOD SAMPLING; CELL LINE; CLINICAL RESEARCH; DONOR; EPSTEIN BARR VIRUS; FLOW CYTOMETRY; HEMATOPOIETIC STEM CELL; HEPATITIS C VIRUS; HLA SYSTEM; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS; LABORATORY; LABORATORY TEST; MYCOPLASMA; PHENOTYPE; PRIORITY JOURNAL; REGISTER; SAFETY; TISSUE TYPING; VIRUS TRANSFORMATION; VOLUNTEER;

B-LYMPHOCYTES; CELL LINE, TRANSFORMED; CELL TRANSFORMATION, VIRAL; FLOW CYTOMETRY; HERPESVIRUS 4, HUMAN; HISTOCOMPATIBILITY TESTING; HLA ANTIGENS; HUMANS; QUALITY CONTROL;

BACTERIA (MICROORGANISMS); DNA VIRUSES; HEPATITIS C VIRUS; HUMAN HERPESVIRUS 4; HUMAN IMMUNODEFICIENCY VIRUS; MYCOPLASMA;

EID: 2342503147     PISSN: 09607420     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2370.2004.00450.x     Document Type: Article

References (20)

1. Advisory Committee on Dangerous Pathogens (2000) Second Supplement (2000) to: Categorisation of Biological Agents According to Hazard and Categories of Containment, 4th edn, 1995. HSE Books, London.
2. Bolton, B. (1996) EBV transformation. In: Cell and Tissue Culture: Laboratory Procedures (ed. by A. Doyle, J. B. Griffiths & D. G. Newell), 26G:1.1. John Wiley & Sons, Chichester.
3. Darke, C. (2000) An overview of the Welsh bone marrow donor registry: 10 years of bone marrow donor provision. Bone Marrow Transplantation, 25, 771.
4. Darke, C., Guttridge, M.G., Thompson, J., McNamara, S., Street, J. & Thomas, M. (1998) HLA class I (A,B) and II (DR,DQ) gene and haplotype frequencies in blood donors from Wales. Experimental and Clinical Immunogenetics, 15, 69.
5. Darke, C., Guttridge, M.G., Street, J., Thompson, J. & Thomas, M. (2000) Molecular, serological and genetic studies on two new HLA-DRB1 alleles - HLA-DRB1*0704 and HLA-DRB1*1507. Tissue Antigens, 56, 467.
6. Darke, C., Street, J. & Downing, J. (2003) Immunogenetics of two new HLA-B alleles: B*4414 and B*5708. Tissue Antigens, 62, 436.
7. Downing, J., Guttridge, M.G. & Darke, C. (2004) Five-locus HLA typing of haematopoietic stem cell donor volunteers by PCR using sequence-specific primers. Genetic Testing, in press.
8. Gold, M. (1986) A Conspiracy of Cells. State University of New York Press, New York.
9. Heads, A.J., Harmer, A.W., Norman, P.J. & Vaughan, R.W. (1993) Rapid antibody screening using EBV cell pools. European Journal of Immunogenetics, 20, 513.
10. Morris, C. (1996) Subculturing. In: Cell and Tissue Culture: Laboratory Procedures (ed. by A. Doyle, J. B. Griffiths & D. G. Newell), 4A:2.1. John Wiley & Sons, Chichester.
11. Mowles, J. & Doyle, A. (1996) Contaminants. In: Cell and Tissue Culture: Laboratory Procedures (ed. by A. Doyle, J. B. Griffiths & D. G. Newell), 7A:1.1. John Wiley & Sons, Chichester.
12. Street, J., Downing, J. & Darke, C. (2003a) Immunogenetics of the rare HLA-B allele B*4408. European Journal of Immunogenetics, 30, 129.
13. Street, J., Downing, J., Hammond, L., Thompson, J. & Darke, C. (2003b) Immunogenetics of two new HLA alleles: A*0108 and B*4031. Tissue Antigens, 61, 322.
14. United Kingdom Co-ordinating Committee on Cancer Research (1999) Guidelines for the Use of Cell Lines in Cancer Research, 1st edn. UKCCSR, London.
15. Walters, R. & Darke, C. (2001) ELISA screening and identification kit findings on cytotoxic negative renal sera. European Journal of Immunogenetics, 28, 493.
16. Walters, R. & Darke, C. (2002) ELISA testing on cytotoxicity negative but flow cytometry positive renal patients' sera. European Journal of Immunogenetics, 29, 363.
17. Walters, R., Coates, E., Rees, T., Bass, H., Winkler, S., Downing, J., Rees, M.T. & Darke, C. (1999) Comprehensive renal patient antibody screening by cytotoxicity, flow cytometry and ELISA. European Journal of Immunogenetics, 26, 58.
18. Walters, R., Williams, H., Downing, J., Hammond, L., Bass, H., Winkler, S. & Darke, C. (2000) Comparison between cytotoxicity, flow cytometry and two ELISA kits for renal patient HLA-class I and II antibody screening. European Journal of Immunogenetics, 27, 290.
19. Walters, R., Manley, C., Harris, E., Harvey, C., Burrows, E.L. & Darke, C. (2003) A study of CDC negative, flow cytometry and ELISA screen positive renal patients' sera. European Journal of Immunogenetics, 30, 320.
20. Yang, S.Y., Milford, E., Hammerling, U. & Dupont, B. (1987) Description of the reference panel of B-lymphoblastoid cell lines for factors of the HLA system. The B-cell line panel designed for the Tenth International Histocompatibility Workshop. In: Immunobiology of HLA, Vol. I, Histocompatibility Testing (ed. by B. Dupont), p. 11. Springer-Verlag, New York.