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Volumn 3, Issue 6, 2003, Pages 479-482

Si nanocolumns as novel nanostructured supports for enzyme immobilization

Author keywords

Biocatalysis; Enzyme Immobilization; Glancing Angle Deposition; Nanocolumns; Nanotechnology; Soybean Peroxidase

Indexed keywords

ENZYME; SILICON;

EID: 2342498609     PISSN: 15334880     EISSN: None     Source Type: Journal    
DOI: 10.1166/jnn.2003.240     Document Type: Article
Times cited : (24)

References (27)
  • 11
    • 2342514979 scopus 로고    scopus 로고
    • note
    • Field emission Scanning Electron Microscope (FESEM) images were obtained on a JEOL JSM-6332 (JEOL USA, Peabody, MA) instrument at an accelerating voltage of 5 kV.
  • 12
    • 2342520874 scopus 로고    scopus 로고
    • note
    • -4 Pa. Electron beam bombardment evaporated pure Si (99.9995%) (Alfa Aesar, Ward Hill, MA) onto the substrate at a rate of 5.5 Å/s.
  • 18
    • 2342586264 scopus 로고    scopus 로고
    • note
    • The silanol functional groups were coupled to APTES with the use of 1% (v/v) solution of APTES in anhydrous toluene at 40°C. After 1 h, excess APTES was removed with toluene (200 ml) and then with a mixture of acetonitrile/water (50%, v/v, 200 ml), and a final wash was completed with excess Milli-Q water (200 ml). The chip was then allowed to dry in air.
  • 19
    • 2342618404 scopus 로고    scopus 로고
    • note
    • 2 projected area on the Si wafer base.
  • 20
    • 2342540630 scopus 로고    scopus 로고
    • note
    • Polymer coupling to APTES-treated supports was achieved by dipping the supports in a 1% (w/v) PMA solution in anhydrous acetone (20 ml) for 10 min. Excess layers of PMA were removed by dipping each support in pure anhydrous acetone three times. SBP (Sigma Chemical Co., St. Louis, MO) was then coupled to the PMA by incubating 1 mg/ml SBP (0.1 M, pH 7.0 phosphate buffer) for 2 h at room temperature. To remove loosely bound SBP, the supports were washed (3 X 1.5 ml) with the same buffer and then incubated in 1.5 ml buffer for 2 h.
  • 24
    • 2342485206 scopus 로고    scopus 로고
    • note
    • The thickness of the layers deposited on silicon wafers was measured after each treatment with an M-44 Ellipsometer (J. A. Woollam Co., Inc., Lincoln, NE). The reported value is an average of three readings obtained at an incident angle of 75°. The values of the refractive index used for APTES, PMA, and SBP were 1.45, 1.51, and 1.51, respectively.
  • 25
    • 2342580335 scopus 로고    scopus 로고
    • note
    • 2 (250 μl, 0.1 M sodium phosphate buffer, pH 7, with 20% (v/v) dimethyl formamide), and the fluorescence was monitored as described above.
  • 26
    • 2342582332 scopus 로고    scopus 로고
    • note
    • 2,2-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) is a sensitive substrate for peroxidase activity and is useful for measuring the activity of sub-ng/ml concentrations of SBP. The initial rate of SBP-catalyzed oxidation of ABTS was measured by monitoring the increase in the absorbance of the end product at 405 nm with a bioassay reader.
  • 27
    • 0000832894 scopus 로고
    • edited by L. B. Wingard, Jr., E. Katchalski-Katzir, and L. Goldstein, Academic Press, New York
    • J.-M. Engasser and C. Horvath, in Applied Biochemistry and Bioengineering, edited by L. B. Wingard, Jr., E. Katchalski-Katzir, and L. Goldstein, Academic Press, New York (1976), Vol. 1, pp. 127-220.
    • (1976) Applied Biochemistry and Bioengineering , vol.1 , pp. 127-220
    • Engasser, J.-M.1    Horvath, C.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.