Resolution of Gsα and Gqα/G 11α proteins in membrane domains by two-dimensional electrophoresis: The effect of long-term agonist stimulation

Physiological Research

Volumn 53, Issue 3, 2004, Pages 295-303

  Matoušek, Petr ^a,b   Novotny J ^a   Svoboda, P ^a  

^a CHARLES UNIVERSITY   (Czech Republic)

^b INSTITUTE OF PHYSIOLOGY   (Czech Republic)

Author keywords

Desensitization; G Proteins; Membrane domains; Rafts; Two dimensional electrophoresis

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; GUANINE NUCLEOTIDE BINDING PROTEIN QALPHA 11ALPHA; ISOPRENALINE; PROTIRELIN RECEPTOR; STIMULATORY GUANINE NUCLEOTIDE BINDING PROTEIN; STIMULATORY GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL COMPOSITION; CONTROLLED STUDY; DOWN REGULATION; DRUG MECHANISM; ELECTROPHORESIS; FLOTATION; GENE OVEREXPRESSION; HORMONAL REGULATION; HUMAN; HUMAN CELL; IMMUNOBLOTTING; LYMPHOMA CELL LINE; MEMBRANE STRUCTURE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN CONTENT; PROTEIN DOMAIN; PROTEIN EXPRESSION; SILVER STAINING; TARGET CELL;

CELL LINE, TUMOR; CELLS, CULTURED; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; GTP-BINDING PROTEIN ALPHA SUBUNITS; HUMANS; ISOPROTERENOL; KIDNEY; LYMPHOMA; MEMBRANE MICRODOMAINS; RECEPTORS, THYROTROPIN-RELEASING HORMONE; RECOMBINANT PROTEINS;

EID: 2342495565     PISSN: 08628408     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (35)

1. ARAGAY AM, KATZ A, SIMON MI: The Gαq and Gα11 proteins couple the thyrotropin-releasing hormone receptor to phospholipase C in GH3 rat pituitary cells. J Biol Chem 267: 24983-24988, 1992.
2. BLUM H, BEIER H, GROSS HJ: Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8: 93-99, 1987.
3. BOUŘOVÁ L, KOSTRNOVÁ A, HEJNOVÁ L, MORAVCOVÁ Z, MOON HE, NOVOTNÝ J, MILLIGAN G, SVOBODA P: Delta-opioid receptors exhibit high efficiency when activating trimeric G proteins in membrane domains. J Neurochem 85: 34-49, 2003.
4. BRAY PF, BARSH G, ROSA JP, LUO XY, MAGENIS E, SHUMAN MA: Physical linkage of the genes for platelet membrane glycoproteins IIb and IIIa. Proc Natl Acad Sci USA 85: 8683-8687, 1988.
5. BROWN DA, ROSE JK: Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface. Cell 68: 533-544, 1992.
6. ČERNÝ J, STOCKINGER H, HOŘEJŠÍ V: Noncovalent associations of T lymphocyte surface proteins. Eur J Immunol 26: 2335-2343, 1996.
7. CINEK T, HOŘEJŠÍ V: The nature of large noncovalent complexes containing glycosyl-phosphatidylinositol-anchored membrane glycoproteins and protein tyrosine kinases. J Immunol 149: 2262-2270, 1992.
8. CODINA J, KIMURA S, KRAUS-FRIEDMANN N: Demonstration of the presence of G-proteins in hepatic microsomal fraction. Biochem Biophys Res Commun 150: 848-852, 1988.
9. DESSY C, KELLY RA, BALLIGAND JL, FERON O: Dynamin mediates caveolar sequestration of muscarinic cholinergic receptors and alteration in NO signalling. EMBO J 19: 4272-4280, 2000.
10. 11α and thyrotropin-releasing hormone receptors proceed on different time scales. J Biol Chem 273: 21699-21707, 1998.
11. 11α induced by agonist stimulation. Biochem J 340: 529-538, 1999.
12. FIEDLER K, KOBAYASHI T, KURZCHALIA TV, SIMONS K: Glycosphingolipid- enriched, detergent-insoluble complexes in protein sorting in epithelial cells. Biochemistry 32: 6365-6373, 1993.
13. GILMAN AG: G proteins: transducers of receptor-generated signals. Annu Rev Biochem 56: 615-649, 1987.
14. HAGA T, HAGA K, GILMAN AG: Hydrodynamic properties of the beta-adrenergic receptor and adenylate cyclase from wild type and variant S49 lymphoma cells. J Biol Chem 252: 5776-5782, 1977.
15. HSI ED, SIEGEL JN, MINAMI Y, LUONG ET, KLAUSNER RD, SAMELSON LE: T cell activation induces rapid tyrosine phosphorylation of a limited number of cellular substrates. J Biol Chem 264: 10836-10842, 1989.
16. KAZIRO Y, ITOH H, KOZASA T, NAKAFUKU M, SATOH T: Structure and function of signal-transducing GTP-binding proteins. Annu Rev Biochem 60: 349-400, 1991.
17. sα gene. Proc Natl Acad Sci USA 85: 2081-2085, 1988.
18. KVAPIL P, NOVOTNY J, SVOBODA P, RANSNAS LA: The short and long forms of the alpha subunit of the stimulatory guanine-nucleotide-binding protein are unequally redistributed during (-)-isoproterenol-mediated desensitisation of intact S49 lymphoma cells. Eur J Biochem 226: 193-199, 1994.
19. 1 receptors translocate out of caveolae. J Biol Chem 275: 4417-4421, 2000.
20. LOWRY OH, ROSENBROUGH NJ, FARR A, RANDALL RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265-275, 1951.
21. 11α proteins from detergent-insensitive membrane domains. FEBS Lett 464: 35-40, 1999.
22. RANSNAS LA, SVOBODA P, JASPER JR, INSEL PA: Stimulation of beta-adrenergic receptors of S49 lymphoma cells redistributes the alpha subunit of the stimulatory G protein between cytosol and membranes. Proc Natl Acad Sci USA 86: 7900-7903, 1989.
23. ROBINSON P, HEDERER R: Early molecular signals induced by antibodies to GPI-anchored proteins. Braz J Med Biol Res 27: 263-267, 1994.
24. ROBISHAW JD, SMIGEL MD, GILMAN AG: Molecular basis for two forms of the G protein that stimulates adenylate cyclase. J Biol Chem 261: 9587-9590, 1986.
25. RODBELL M: The role of hormone receptors and GTP-regulatory proteins in membrane transduction. Nature 284: 17-22, 1980.
26. RYBIN VO, XU X, LISANTI MP, STEINBERG SF: Differential targeting of beta-adrenergic receptor subtypes and adenylyl cyclase to cardiomyocyte caveolae. A mechanism to functionally regulate the cAMP signalling pathway. J Biol Chem 275: 41447-41457, 2000.
27. SHENOY-SCARIA AM, GAUEN LK, KWONG J, SHAW AS, LUBLIN DM: Palmitylation of an amino-terminal cysteine motif of protein tyrosine kinases p56lck and p59fyn mediates interaction with glycosyl-phosphatidylinositol-anchored proteins. Mol Cell Biol 13: 6385-6392, 1993.
28. SIMON MI, STRATHMANN MP, GAUTAM N: Diversity of G proteins in signal transduction. Science 252: 802-808, 1991.
29. SOLOMON KR, RUDD CE, FINBERG RW: The association between glycosylphosphatidylinositol-anchored proteins and heterotrimeric G protein alpha subunits in lymphocytes. Proc Natl Acad Sci USA 93: 6053-6058, 1996.
30. SONG KS, LI S, OKAMOTO T, QUILLIAM LA, SARGIACOMO M, LISANTI MP: Co-purification and direct interaction of Ras with caveolin, an integral membrane protein of caveolae microdomains. Detergent-free purification of caveolae microdomains. J Biol Chem 271: 9690-9697, 1996a.
31. SONG KS, SCHERER PE, TANG Z, OKAMOTO T, LI S, CHAFEL M, CHU C, KOHTZ DS, LISANTI MP: Expression of caveolin-3 in skeletal, cardiac, and smooth muscle cells. Caveolin-3 is a component of the sarcolemma and co-fractionates with dystrophin and dystrophin-associated glycoproteins. J Biol Chem 271: 15160-15105, 1996b.
32. STRYER L, BOURNE HR: G proteins: a family of signal transducers. Annu Rev Cell Biol 2: 391-419, 1986.
33. SVOBODA P, KVAPIL P, INSEL PA, RANSNAS LA: Plasma-membrane-independent pool of the α subunit of the stimulatory guanine-nucleotide-binding regulatory protein in low-density-membrane fraction of S49 lymphoma cells. Eur J Biochem 208: 693-698, 1992.
34. 11α species variants. Mol Pharmacol 49: 646-655, 1996.
35. TANG WJ, GILMAN AG: Adenylyl cyclases. Cell 70: 869-872, 1992.