Achievement of High Recovered Activity and Efficient Purification and Recovery by Application of Immobilized Inhibitor to Protease Refolding

Kagaku Kogaku Ronbunshu

Volumn 27, Issue 2, 2001, Pages 169-173

  Nohara, Daisuke ^a   Kondoh, Daisuke ^a   Sakai, Tomoya ^a  

^a NAGOYA CITY UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 23044526991     PISSN: 0386216X     EISSN: 13499203     Source Type: Journal    
DOI: 10.1252/kakoronbunshu.27.169     Document Type: Article

References (20)

1. Anfinsen, C. B.; Principles that Govern the Folding of Protein Chains” Science, 181, 223-230 (1973)
2. Harris, T. J. R.; Expression of Eukaryotic Genes in E. coli” Genet. Eng., 4, 127-185 (1983)
3. Hayashi, T., M. Matsubara, E. Kurimoto, D. Nohara and T. Sakai; Refolding of Subtilisin BPN' Achieved Almost Quantitatively by Covalent Immobilization on an Agarose Gel” Chem. Pharm. Bull., 41, 2063-2065 (1993)
4. Hayashi, T., M. Matsubara, D. Nohara, S. Kojima, K. Miura and T. Sakai; Renaturation of the Mature Subtilisin BPN' Immobilized Agarose Beads” FEES Lett., 350, 109-112 (1994)
5. Marston, F. A. O.; The Purification of Eukaryotic Polypeptides Synthesized in Escherichia coli,'' Biochem. J., 240, 1-12 (1986)
6. Matsubara, M., E. Kurimoto, S. Kojima, K. Miura and T. Sakai; Quantitative in vitro Renaturation of Subtilisin BPN' without the Aid of Prosequence” Chem. Lett., 1783-1766 (1993 a)
7. Matsubara, M., D. Nohara, E. Kurimoto, Y. Kuroda and T. Sakai; Loose Folding and Delayed Oxidation Procedures Successfully Applied for Refolding of Fully Reduced Hen Egg White Lysozyme” Chem. Pharm. Bull., 41, 1207-1210 (1993 b)
8. Matsubara, M., E. Kurimoto, S. Kojima, K. Miura and T. Sakai; Achievement of Renaturation of Subtilisin BPN' by a Novel Procedure Using Organic Salts and a Digestible Mutant of Streptomyces Subtilisin Inhibitor” FEBS Lett., 342, 193-196 (1994)
9. Misawa, S. and I. Kumagai; Refolding of Therapeutic proteins Produced in Escherichia coli as Inclusion Bodies” Biopolymers, 51, 297-307 (1999)
10. Mitraki, A. and J. King; Protein Folding Intermediates and Inclusion Body Formation” Bio/Technol., 7, 690-697 (1989)
11. Nohara, D., M. Matsubara, K. Tano and T. Sakai; Design of Optimum Refolding Solution by Combination of Reagents Classified by Specific Function” J. Ferment. Bioeng., 82, 401-403 (1996 a)
12. Nohara, D. and T. Sakai; Fundamental Conditionings for Refolding of Denatured Proteins” Recent Res. Devel. in Chem. & Pharm. Sci., 1, 79-89 (1996)
13. Nohara, D. and T. Sakai; Recent Developments in Media Selection for Protein Refolding” Recent Devel. in Ferment. & Bioeng., 1, 165-173 (1998)
14. Nohara, D., H. Sakakibara, E. Kurimoto, T. Hayashi and T. Sakai; A New Method of Media Selection for Protease Refolding by Application of Immobilized Subtilisin Preparation” J. Ferment. Bioeng., 82, 315-318 (1996 b)
15. Nohara, D., Y. Senga, M. Matsubara and T. Sakai; Media Selection for Refolding of Thermolysin by Use of Immobilized Preparation” J. Biosci. Bioeng., 89, 188-192 (2000)
16. Nohara, D., H. Sugiura, H. Sakakibara, M. Matsubara, S. Kojima, K. Miura and T. Sakai; High Performance in Refolding of Streptomyces griseus Trypsin by the Aid of a Mutant of Streptomyces Subtilisin Inhibitor Designed as Trypsin Inhibitor” J. Biochem., 125, 343-347 (1999)
17. Olafson, R. W. and L. B. Smillie; Enzymic and Physicochemical Properties of Streptomyces griseus Trypsin” Biochemistry, 14, 1161-1167 (1975)
18. Rudolph, R. and H. Lilie; ''In vitro Folding of Inclusion Body Proteins” FASEB J., 10, 49-56 (1996)
19. Yokosawa, H., T. Hanba and S. Ishii; Affinity Chromatography of Trypsin and Related Enzymes” J. Biochem., 79, 757-763 (1976)
20. Zako, T., H. Deguchi, A. Kitayama, H. Ueda and T. Nagamune; Refolding of Firefly Luciferase Immobilized on Agarose Beads” J. Biochem., 127, 351-354 (2000)