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Volumn 82, Issue 3, 1996, Pages 315-318

A new method of media selection for protease refolding by application of immobilized subtilisin preparation

Author keywords

immobilized preparation; protein refolding; refolding media selection; subtilisin BPN

Indexed keywords

PROTEINASE; SUBTILISIN;

EID: 0029976866     PISSN: 0922338X     EISSN: None     Source Type: Journal    
DOI: 10.1016/0922-338X(96)88827-1     Document Type: Article
Times cited : (10)

References (14)
  • 1
    • 0017697097 scopus 로고
    • Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin
    • Itakura, K., Hirose, T., Crea, R., Riggs, A., Heyneker, H., Bolivar, F., and Boyer, H.: Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin. Science, 198, 1056-1063 (1977).
    • (1977) Science , vol.198 , pp. 1056-1063
    • Itakura, K.1    Hirose, T.2    Crea, R.3    Riggs, A.4    Heyneker, H.5    Bolivar, F.6    Boyer, H.7
  • 2
    • 0019466154 scopus 로고
    • Stabilization of a degradable protein by its overexpression in Escherichia coli
    • Cheng, Y., Kwoh, D., Kwoh, T., Soltvedt, B., and Zipser, D.: Stabilization of a degradable protein by its overexpression in Escherichia coli. Gene, 14, 121-130 (1981).
    • (1981) Gene , vol.14 , pp. 121-130
    • Cheng, Y.1    Kwoh, D.2    Kwoh, T.3    Soltvedt, B.4    Zipser, D.5
  • 3
    • 0020036666 scopus 로고
    • Cytoplasmic inclusion bodies in Escherichia coli producing biosynthetic human insulin proteins
    • Williams, D., Frank, R., Muth, W., and Burnett, J.: Cytoplasmic inclusion bodies in Escherichia coli producing biosynthetic human insulin proteins. Science, 215, 687-689 (1982).
    • (1982) Science , vol.215 , pp. 687-689
    • Williams, D.1    Frank, R.2    Muth, W.3    Burnett, J.4
  • 4
    • 0026301852 scopus 로고
    • Protein folding and its implications for the production of recombinant proteins
    • Hlodan, R., Craig, S., and Pain, R. H.: Protein folding and its implications for the production of recombinant proteins. Biotechnol. Genet. Eng. Rev., 9, 47-88 (1991).
    • (1991) Biotechnol. Genet. Eng. Rev. , vol.9 , pp. 47-88
    • Hlodan, R.1    Craig, S.2    Pain, R.H.3
  • 5
    • 0013559198 scopus 로고
    • Thermal deactivation and subsequent reactivation of enzymes in connection with precipitates formation. A survey of correct refolding
    • Sakai, T., Mizutani, A., Yamada, T., and Nohara, D.: Thermal deactivation and subsequent reactivation of enzymes in connection with precipitates formation. A survey of correct refolding. Prot. Eng., 3, 368-369 (1990).
    • (1990) Prot. Eng. , vol.3 , pp. 368-369
    • Sakai, T.1    Mizutani, A.2    Yamada, T.3    Nohara, D.4
  • 6
    • 0343553876 scopus 로고
    • Quantitative in vitro renaturation of subtilisin BPN' without the aid of pro-sequence
    • Matsubara, M., Kurimoto, E., Kojima, S., Miura, K., and Sakai, T.: Quantitative in vitro renaturation of subtilisin BPN' without the aid of pro-sequence. Chem. Lett., 1783-1786 (1993).
    • (1993) Chem. Lett. , pp. 1783-1786
    • Matsubara, M.1    Kurimoto, E.2    Kojima, S.3    Miura, K.4    Sakai, T.5
  • 7
    • 0017156362 scopus 로고
    • Denaturation of subtilisin BPN' and its derivatives in aqueous guanidine hydrochloride solutions
    • Ikai, A.: Denaturation of subtilisin BPN' and its derivatives in aqueous guanidine hydrochloride solutions. Biochim. Biophys. Acta, 445, 182-193 (1976).
    • (1976) Biochim. Biophys. Acta , vol.445 , pp. 182-193
    • Ikai, A.1
  • 8
    • 0024409494 scopus 로고
    • Pro-sequence of subtilisin can guide the refolding of denatured subtilisin in an intermolecular process
    • Zhu, X., Ohta, Y., Jordan, F., and Inouye, M.: Pro-sequence of subtilisin can guide the refolding of denatured subtilisin in an intermolecular process. Nature, 339, 483-484 (1989).
    • (1989) Nature , vol.339 , pp. 483-484
    • Zhu, X.1    Ohta, Y.2    Jordan, F.3    Inouye, M.4
  • 9
    • 0026168310 scopus 로고
    • Pro-peptide as an intramolecular chaperone: Renaturation of denatured subtilisin E with a synthetic pro-peptide
    • Ohta, Y., Hojo, H., Aimoto, S., Kobayashi, T., Zhu, X., Jordan, F., and Inouye, M.: Pro-peptide as an intramolecular chaperone: renaturation of denatured subtilisin E with a synthetic pro-peptide. Mol. Microbiol., 5, 1507-1510 (1991).
    • (1991) Mol. Microbiol. , vol.5 , pp. 1507-1510
    • Ohta, Y.1    Hojo, H.2    Aimoto, S.3    Kobayashi, T.4    Zhu, X.5    Jordan, F.6    Inouye, M.7
  • 10
    • 0027464607 scopus 로고
    • Folding of subtilisin BPN': Characterization of a folding intermediate
    • Eder, J., Rheinnecker, M., and Fersht, A. R.: Folding of subtilisin BPN': characterization of a folding intermediate. Biochemistry, 32, 18-26 (1993).
    • (1993) Biochemistry , vol.32 , pp. 18-26
    • Eder, J.1    Rheinnecker, M.2    Fersht, A.R.3
  • 11
    • 0027764240 scopus 로고
    • Refolding of subtilisin BPN' achieved almost quantitatively by covalent immobilization on an agarose gel
    • Hayashi, T., Matsubara, M., Kurimoto, E., Nohara, D., and Sakai, T.: Refolding of subtilisin BPN' achieved almost quantitatively by covalent immobilization on an agarose gel. Chem. Pharm. Bull., 41, 2063-2065 (1993).
    • (1993) Chem. Pharm. Bull. , vol.41 , pp. 2063-2065
    • Hayashi, T.1    Matsubara, M.2    Kurimoto, E.3    Nohara, D.4    Sakai, T.5
  • 12
    • 0028326969 scopus 로고
    • Achievement of renaturation of subtilisin BPN' by a novel procedure using organic salts and a digestible mutant of Streptomyces subtilisin inhibitor
    • Matsubara, M., Kurimoto, E., Kojima, S., Miura, K., and Sakai, T.: Achievement of renaturation of subtilisin BPN' by a novel procedure using organic salts and a digestible mutant of Streptomyces subtilisin inhibitor. FEBS Lett., 342, 193-196 (1994).
    • (1994) FEBS Lett. , vol.342 , pp. 193-196
    • Matsubara, M.1    Kurimoto, E.2    Kojima, S.3    Miura, K.4    Sakai, T.5
  • 13
    • 0028103878 scopus 로고
    • Renaturation of the mature subtilisin BPN' immobilized on agarose beads
    • Hayashi, T., Matsubara, M., Nohara, D., Kojima, S., Miura, K., and Sakai, T.: Renaturation of the mature subtilisin BPN' immobilized on agarose beads. FEBS Lett., 350, 109-112 (1994).
    • (1994) FEBS Lett. , vol.350 , pp. 109-112
    • Hayashi, T.1    Matsubara, M.2    Nohara, D.3    Kojima, S.4    Miura, K.5    Sakai, T.6
  • 14
    • 0018746145 scopus 로고
    • Affinity chromatography on immobilized hyaluronate and its application to the isolation of hyaluronate binding proteins from cartilage
    • Tengblad, A.: Affinity chromatography on immobilized hyaluronate and its application to the isolation of hyaluronate binding proteins from cartilage. Biochim. Biophys. Acta, 578, 281-289 (1979).
    • (1979) Biochim. Biophys. Acta , vol.578 , pp. 281-289
    • Tengblad, A.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.